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  • auxin conjugates  (2)
  • Enzyme digestion
  • LUNAR AND PLANETARY EXPLORATION
  • Springer  (3)
  • 2010-2014
  • 1990-1994  (2)
  • 1975-1979  (1)
  • 1950-1954
Sammlung
Verlag/Herausgeber
  • Springer  (3)
Erscheinungszeitraum
  • 2010-2014
  • 1990-1994  (2)
  • 1975-1979  (1)
  • 1950-1954
Jahr
  • 1
    Digitale Medien
    Digitale Medien
    Springer
    Plant growth regulation 13 (1993), S. 189-195 
    ISSN: 1573-5087
    Schlagwort(e): Arabidopsis thaliana ; autofluorography ; auxin conjugates ; IBA-glucose synthase ; indole-3-butyric acid
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Abstract Indole-3-butyric acid (IBA) was metabolized by seedlings of Arabidopsis thaliana cultivated in liquid medium under sterile conditions to two major metabolites. One metabolite was hydrolyzed by 1 N NaOH and β-glucosidase and was tentatively identified as IBA-glucose and the other was hydrolyzed by 7 N NaOH and amidase and was identified as an amide-linked conjugate. IBA-glucose synthase activity was found in a soluble enzyme fraction after incubation of 3H-IBA, IBA and UDP-glucose. The labelled reaction product had an Rf value comparable to IBA glucose and stained positive with Ehmann reagent.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 2
    Digitale Medien
    Digitale Medien
    Springer
    Plant growth regulation 13 (1993), S. 179-187 
    ISSN: 1573-5087
    Schlagwort(e): Arabidopsis thaliana ; auxin conjugates ; ethylene ; indole-3-acetic acid ; indole-3-butyric acid
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Abstract Indole-3-butyric acid (IBA) was identified by HPLC and GC-MS as an endogenous compound in plantlets of the crucifer Arabidopsis thaliana (L.) Heynh. A. thaliana was cultivated under sterile conditions as shaking culture in different liquid media with and without supply of hormones. Free and total IBA and indole-3-acetic acid (IAA) were determined at different stages of development during the culture period as well as in culture media of different initial pH values. The results showed that IAA was present in higher concentrations than IBA, but both hormones seemed to show the same behaviour under the different experimental conditions. Differences were found in the mode of conjugation of the two hormones. While IAA was mostly conjugated via amide bonds, the main IBA conjugates were ester bound. The ethylene concentration derived from the seedlings, when they were grown in flasks of different size, seemed not to influence the auxin content in the same cultures.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 3
    Digitale Medien
    Digitale Medien
    Springer
    Cell & tissue research 184 (1977), S. 155-167 
    ISSN: 1432-0878
    Schlagwort(e): Epidermal cell differentiation ; DNA and RNA ; GMA embedded tissue ; Enzyme digestion ; Schiff-Thallium reaction
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary The distribution of nucleic acids within nuclei of epidermal cells in skin from guinea-pig ear was investigated using an indirect enzyme digestion technique to observe both DNA and RNA, and a direct Schiff-Thallium reaction technique, to observe DNA alone. Similar results were obtained by both methods. The distribution of DNA and RNA change gradually in nuclei as epidermal cells differentiate. DNA in cells of the lower strata is localized in essentially the same areas in which electron-opaque components are seen by conventional electron microscopy. With the cytochemical treatments, however, we found that DNA is not present in all electron-opaque areas of nuclei in superficial granular cells. RNA is present in the nucleoli of cells in all layers, but its density is also lower in the upper granular cells. We postulate that nucleic acids in nuclei of granular cells gradually decrease and that the space is filled with newly synthesized electron-dense protein, as part of the differentiation process.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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