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  • American Chemical Society  (51)
  • American Physical Society  (22)
  • Wiley-Blackwell  (8)
  • 2010-2014  (51)
  • 1995-1999  (30)
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Keywords
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Years
Year
  • 1
    Electronic Resource
    Electronic Resource
    Allosteric Modulation of Acetylcholinesterase Activity by Peripheral Ligands Involves a Conformational Transition of the Anionic Subsite (1995)
    s.l. : American Chemical Society
    Biochemistry 34 (1995), S. 15444-15452 
    Details
    ISSN: 1520-4995
    Source: ACS Legacy Archives
    Topics: Biology , Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/bi00047a008
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  • 2
    Electronic Resource
    Electronic Resource
    Synthesis of Laurencione, a Labile Dihydro-3(2H)-furanone Derivative from the Red Alga Laurencia spectabilis (1995)
    s.l. : American Chemical Society
    The @journal of organic chemistry 60 (1995), S. 5262-5265 
    Details
    ISSN: 1520-6904
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/jo00121a051
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  • 3
    Electronic Resource
    Electronic Resource
    Functionalization of isotactic poly(propylene) with maleic anhydride in the solid phase (1996)
    Weinheim : Wiley-Blackwell
    Angewandte Makromolekulare Chemie 243 (1996), S. 57-67 
    Details
    ISSN: 0003-3146
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Physics
    Description / Table of Contents: Der Einfluß der Temperatur und der Art des Initiators auf den Wirkungsgrad der Pfropfung von Maleinsäureanhydrid auf Polypropylen wurde untersucht. Es wurde festgestellt, daß aliphatische Peroxide wie z. B. Lauroylperoxid ungefähr um 20% effektiver sind als Dibenzoylperoxid, Dicumylperoxid, 2,5-Dimethyl-2,5-di-tert-butylperoxyhexan oder Peroxybenzoesäure-tert-butylester.Der höhere Wirkungsgrad der Pfropfung von Maleinsäureanhydrid auf Polyethylen im Vergleich zu Polypropylen kann mit der längeren kinetischen Kette der Additionsreaktion bei Polyethylen erklärt werden.
    Notes: The influence of temperature and type of initiator on the grafting efficiency of maleic anhydride onto isotactic poly(propylene) powder under solid-state reaction conditions was investigated. The level of bonded maleic anhydride was found to be about 20% higher using aliphatic peroxide, such as lauroyl peroxide, in contrast to dibenzoyl peroxide, dicumyl peroxide, 2,5-dimethyl-2,5-di-tert-butylperoxyhexane or tert-butyl perbenzoate as initiator.The higher grafting efficiency of maleic anhydride onto polyethylene when compared with poly(propylene) is accounted for by a higher kinetic length of the addition-transfer chain reactions.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/apmc.1996.052430105
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  • 4
    Electronic Resource
    Electronic Resource
    Localization of group IIc low molecular weight phospholipase A2 mRNA to meiotic cells in the mouse (1997)
    New York, N.Y. : Wiley-Blackwell
    Journal of Cellular Biochemistry 64 (1997), S. 369-375 
    Details
    ISSN: 0730-2312
    Keywords: testis ; phospholipase A2 ; cDNA sequence ; in situ hybridization ; mouse ; pla2g2c ; spermatocytes ; meiosis ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: We use in situ hybridization to demonstrate that the testicular expression of a novel, mouse, low molecular weight phospholipase A2 (PLA2 Group IIc) mRNA is specific to cells undergoing meiosis. A complete cDNA (1421 bp) encoding the mouse Pla2g2c gene was generated with reverse transcription-PCR (RT-PCR) and 5′ and 3′ RACE (rapid amplification of cDNA ends) RT-PCR, and its nucleotide sequence was determined. Northern blots of RNA from different tissues revealed a single 1.6 kb transcript only in testis. In situ hybridization indicated that this mouse gene is transcribed mainly in pachytene spermatocytes, secondary spermatocytes, and round spermatids. Expression of the gene is seen in all stages of the seminiferous epithelium, especially in stages VI-VII. J. Cell. Biochem. 64:369-375. © 1997 Wiley-Liss, Inc.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Hollow fiber bioartificial liver utilizing collagen-entrapped porcine hepatocyte spheroids (1996)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 52 (1996), S. 34-44 
    Details
    ISSN: 0006-3592
    Keywords: hepatocyte spheroid ; porcine hepatocyte ; hollow fiber ; bioartificial liver ; collagen ; bioreactor ; ureagenesis ; albumin synthesis ; glucuronidation ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A xenogeneic hollow fiber bioreactor utilizing collagen-entrapped dispersed hepatocytes has been developed as an extracorporeal bioartificial liver (BAL) for potential treatment of acute human fulminant hepatitis. Prolonged viability, enhanced liver-specific functions, and differentiated state have been observed in primary porcine hepatocytes cultivated as spheroids compared to dispersed hepatocytes plated on a monolayer. Entrapment of spheroids into the BAL can potentially improve performance over the existing device. Therefore, studies were conducted to evaluate the feasibility of utilizing spheroids as the functionally active component of our hybrid device. Confocal microscopy indicated high viability of spheroids entrapped into cylindrical collagen gel. Entrapment of spheroids alone into collagen gel showed reduced ability to contract collagen gel. By mixing spheroids with dispersed cells, the extent of collagen gel contraction was increased. Hepatocyte spheroids collagen-entrapped into BAL devices were maintained for over 9 days. Assessment of albumin synthesis and ureagenesis within a spheroid-entrapment BAL indicated higher or at least as high activity on a per-cell basis compared to a dispersed hepatocyte-entrapment BAL device. Clearance of 4-methylumbelliferone to its glucuronide was detected throughout the culture period as a marker of phase II conjugation activity. A spheroid-entrapment bioartificial liver warrants further studies for potential human therapy. © 1996 John Wiley & Sons, Inc.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Cross-linking of poly(methyl methacrylate) by aminolysis of ester functions with diamines (1995)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Applied Polymer Science 55 (1995), S. 39-45 
    Details
    ISSN: 0021-8995
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: The cross-linking of poly(methyl methacrylate) (PMMA) macromolecules by primary aliphatic diamines was carried out at 413-473 K, above the polymer glass transition temperature. The spherical grains of the suspension-prepared polymer (0.25-0.8 mm) and the molar ratio to amine above 20 were used. The reaction is characterized by an induction period before the macroscopic gel was observed and by fast network formation. Both phenomena are temperature-dependent (87± 2 kJ mol-1). The rate of gelation is dependent also on the type of diamine; the shorter induction period of gelation and the higher rate of cross-linking were observed when amino groups were separated with longer alkyl chains. From the kinetics of amine uptake with PMMA, however, it can be concluded that gel formation is influenced also by the reactivity of amine in the transamination reaction and by the amount of bounded diamine. Aminolysis of PMMA ester groups proceeds from the very beginning of heating of the reaction charge. The polymer cross-linking prevails when there is more than 60% of diamine attached to PMMA in dependence on the amine type used. © 1995 John Wiley & Sons, Inc.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/app.1995.070550104
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  • 7
    Electronic Resource
    Electronic Resource
    Tautomerism of some amino diol and amino alcohol derivatives in the gas, phase1 (1995)
    New York, NY : Wiley-Blackwell
    Rapid Communications in Mass Spectrometry 9 (1995), S. 916-920 
    Details
    ISSN: 0951-4198
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Physics
    Notes: The mass spectrometric behaviour of condensation products of nine substituted benzaldehydes with 3-amino-1,2-propanediol and 3-amino-1-phenoxy-2-propanol was studied under electron impact and chemical ionization. The 70 eV electron impact mass spectra showed that, as in solution, the amino diol derivatives existed as three different structural isomers in the gas phase: viz. open-chain Schiff base, oxazolidine and tetrahydro-1,3-oxazine. The fragmentations revealed a large amount of the decomposed molecular ions to have the open-chain structure, but also that the amount of both ring forms was considerable. In the same way, with the amino alcohol derivatives both the open-chain and the oxazolidine ring forms were present in the gas phase. In all cases, the ring formation was much more favourable in the gas phase than in solution. Substituents at the phenyl ring caused changes in the relative amounts of the different forms: electron-withdrawing substituents shifted the equilibria in favour of the ring forms while electron-donating substituents favoured the open-chain form. Under chemical ionization, methane, isobutane and acetone were used as reagent gas. Methane was the only reagent gas that led to some fragmentation of the protonated molecules.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/rcm.1290091010
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  • 8
    Electronic Resource
    Electronic Resource
    Electron ionization mass spectrometry of some substituted, stereoisomeric, partly saturated 1,3- and 3,1-benzoxazino-1,3-benzoxazinesSaturated Heterocycles, Part 232. (1995)
    New York, NY : Wiley-Blackwell
    Rapid Communications in Mass Spectrometry 9 (1995), S. 1035-1037 
    Details
    ISSN: 0951-4198
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Physics
    Notes: The electron ionization mass spectra of five substituted, partly saturated 3,1-benzoxazino-1,3-benzoxazines and four substituted 1,3-benzoxazino-1,3-benzoxazines were measured and analysed. The fragmentation pathways were elucidated by metastable ion analysis and exact mass measurement. Although the principal fragmentations were similar for all these compounds, there was an important difference between the two groups as concerns the fragmentation routes. Both routes led to the same fragment ion, but in different ways depending on the ring structure. On the basis of the different fragmentation mechanisms, the 3,1-benzoxazino-1,3-benzoxazines and the 1,3-benzoxazino-1,3-benzoxazines can be distinguished mass spectrometrically. The saturation, the stereochemistry and the substituents all affected the fragmentations, mainly the peak intensities.
    Additional Material: 1 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/rcm.1290091113
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  • 9
    Electronic Resource
    Electronic Resource
    Transcriptional up-regulation of the mouse gene for the muscle-specific subunit of enolase during terminal differentiation of myogenic cells (1995)
    New York, NY [u.a.] : Wiley-Blackwell
    Molecular Reproduction and Development 41 (1995), S. 306-313 
    Details
    ISSN: 1040-452X
    Keywords: β-enolase ; Insulin-like growth factor-II ; Myogenesis in culture ; Gene expression regulation ; 4-Thiouridine labeled RNA isolation ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: The glycolytic enzyme enolase (EC 4.2.1.11) exists as dimers formed from three structurally related subunits α, β, and γ, encoded by separate genes. The gene encoding the β-subunit is expressed only in striated muscles. We have previously shown that the β-enolase gene belongs to a small subset of muscle-specific genes showing transcriptional activity in cultured myoblasts, prior to withdrawal from the cell cycle. An increase in the level of β-enolase mRNA occurs during terminal differentiation of myoblasts. To investigate the mechanisms underlying this increase, we have simultaneously estimated, under steady state conditions, the rate of synthesis and the stability of β-enolase mRNA in proliferating C2.7 myoblasts as well as in differentiating myotubes. The method used is based on the isolation of newly synthesized RNA from the total RNA pool, following pulse-labeling of intact cells in the presence of 4-thiouridine. The results described here demonstrate a coordinate increase in newly synthesized and total β-enolase mRNA, while the mRNA half-life, about 4 hr, remains unchanged in the course of terminal differentiation. The expression of the gene for insulin-like growth factor-II (IGF-II), a major positive regulator of myogenesis, was analyzed using the same approach.It is concluded that the up-regulation of β-enolase as well as IGF-II gene expression in differentiating muscle cells reflects an increased rate of entry of newly synthesized mRNAs into the general pool of transcripts without changes in their respective half-lives. © 1995 Wiley-Liss, Inc.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/mrd.1080410305
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  • 10
    Electronic Resource
    Electronic Resource
    Electron ionization mass spectra of some substituted stereoisomeric 1,6,7,11b-tetrahydro-2h,4H[1,3]oxazino[4,3-a]-isoquinolines and 1,6,7,11b-tetrahydro-2H[1,3]oxazino [4,3-a]-isoquinolin-4-ones1 (1995)
    New York, NY : Wiley-Blackwell
    Rapid Communications in Mass Spectrometry 9 (1995), S. 998-1002 
    Details
    ISSN: 0951-4198
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Physics
    Notes: The mass spectra often substituted oxazino[4,3-a]isoquinolines, two isoquino[2,1-c][1,3]benzoxazines and seven substituted oxazino[4,3-a]isoquinolin-4-ones were recorded under electron impact ionization. Fragmentations were examined by metastable ion analysis, collision induced dissociation and exact mass measurement. The oxazinoisoquinolines and oxazinoisoquinolin-4-ones behaved similarly, although there were a few differences in the fragmentation and especially in the peak intensities. The most important fragmentation began with the opening of the oxazole ring. The substituents affected the fragmentation of the isomeric compounds, whereas the spectra of the stereoisomeric compounds were identical. The two isoquinobenzoxazines differed noticeably from the other compounds in their fragmentation behaviour; the additional phenyl ring prompted entirely new fragmentations unique to this structure.
    Additional Material: 1 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/rcm.1290091105
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