Publikationsdatum:
2014-03-29
Beschreibung:
In many metazoans, germ cells are separated from somatic lineages early in development and maintain their identity throughout life. Here, we show that a Polycomb group (PcG) component, Enhancer of Zeste [E(z)], a histone transferase that generates trimethylation at lysine 27 of histone H3, maintains germline identity in Drosophila adult testes. We find excessive early-stage somatic gonadal cells in E(z) mutant testes, which originate from both overproliferative cyst stem cells and germ cells turning on an early-stage somatic cell marker. Using complementary lineage-tracing experiments in E(z) mutant testes, a portion of excessive early-stage somatic gonadal cells are found to originate from early-stage germ cells, including germline stem cells. Moreover, knocking down E(z) specifically in somatic cells caused this change, which suggests a non-cell autonomous role of E(z) to antagonize somatic identity in germ cells.〈br /〉〈br /〉〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4040133/" target="_blank"〉〈img src="https://static.pubmed.gov/portal/portal3rc.fcgi/4089621/img/3977009" border="0"〉〈/a〉 〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4040133/" target="_blank"〉This paper as free author manuscript - peer-reviewed and accepted for publication〈/a〉〈br /〉〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Eun, Suk Ho -- Shi, Zhen -- Cui, Kairong -- Zhao, Keji -- Chen, Xin -- R00 HD055052/HD/NICHD NIH HHS/ -- R00HD055052/HD/NICHD NIH HHS/ -- R01 HD065816/HD/NICHD NIH HHS/ -- R01HD065816/HD/NICHD NIH HHS/ -- Intramural NIH HHS/ -- New York, N.Y. -- Science. 2014 Mar 28;343(6178):1513-6. doi: 10.1126/science.1246514.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Biology, The Johns Hopkins University, Baltimore, MD 21218, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/24675960" target="_blank"〉PubMed〈/a〉
Schlagwort(e):
Animals
;
Biomarkers/metabolism
;
Cell Differentiation
;
Cell Lineage
;
Drosophila Proteins/genetics/*physiology
;
Drosophila melanogaster/cytology/*growth & development
;
Male
;
Nuclear Proteins/genetics/*physiology
;
Polycomb Repressive Complex 2/genetics/*physiology
;
Spermatocytes
;
Spermatogonia/*metabolism
;
Testis/cytology/*growth & development
Print ISSN:
0036-8075
Digitale ISSN:
1095-9203
Thema:
Biologie
,
Chemie und Pharmazie
,
Informatik
,
Medizin
,
Allgemeine Naturwissenschaft
,
Physik
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