ALBERT

Description: The changes in chemical, microbial and quality sensory of fillets for silver carp fish treated with black cumin extract during refrigerated storage were investigated. The fish were divided into two groups. First group was dipped in black cumin extract with concentration of 1% and received the highest score in sensory evaluation. Then the fishes were wrapped in polyethylene package. The second group, as the control samples, were wrapped in polyethylene package after dipping in distilled water. All treatments were stored at refrigerator temperature (1±4°C). The microbial tests including total viable count, psychrotrophic count and chemical tests including peroxide index, thiobarbituric acid, free fatty acid value and total volatile basic nitrogen, with sensory evaluation, were done at 4°C over a period of 15 days. The results showed that the black cumin extract delayed lipid oxidation and protein analyses significantly in treated fishes. Psycrotrophic bacteria and total viable count of samples being treated with black cumin extract were maintained lower than the proposed acceptable limit (7 log cfu/g). In comparison to the control samples, microbial spoilage significantly decreased in treated samples. Furthermore, according to sensory analysis, the treatment with black cumin extract led to high quality during storage.The findings indicated that black cumin exerts had strong antioxidant and antibacterial impacts on silver carp fish, such that the shelf life of fillets being treated with black cumin were 2.5 times more than that of control samples during storage in refrigerator.

Description: Published

Description: In the present study, hydrolysed protein of viscera and head of Silver carp (Hypophthalmichthys molitrix) was compared using Alcalase enzyme and internal tissue enzymes at 2 and 4 hours. The result indicated that product by Alcalase (Treatment 1) had significantly higher protein and rate of hydrolysates than that produced by internal tissue enzymes (Treatment 2). So, the highest mean (±SD) protein (68.10±1.33) was related to treatment 1-head (with Alcalase enzyme) after 4 hours and the highest rate of hydrolysates (29.36±1.35) was related to treatment 1-head (with Alcalase enzyme) after 4 hours. The result indicated that rate of hydrolysates raised as time of hydrolysates increased. However the intensity and rate of hydrolysates is reduced. The highest rate of hydrolysates occurred at 120 minutes in the first. This mode was similar for two treatments. The result can be considered as the Alcalase was preferred to internal enzyme.

Description: Published

Description: breeding has moved beyond the point of simply propagating random stock. Genetic potential can be improved by establishing and crossing genetically defined strains, crossing species and manipulating gametes. Some of these activities require cryopreservation of semen. Cryopreserved sperm could also be a means of exchange of genetic material between locations and populations when transportation of live fish is restricted. In this study we had a survey on rainbow trout sperm quality in Genetic and Breeding Center for Coldwatwr Fishes, Shahid Motahari, Yasouj. Then Cryopreservation trials on rainbow trout (Oncorhynchus mykiss) sperm were carried out using six basic extenders. Egg batches of 25 g were inseminated with semen frozen in five 0.5-ml straws. Thowing temperature of cryopreserved sperm also was tested. In anather trial we investigate different cryoprotectant. The result showd that the best period for sperm collection in this center was from Novamber to January. The best extender based on sperm motility parameters were extender 2 and 1 with 57.2 and 56.9% fertilisatin rates, respectively. So this two extender were used in the further experiments. The best thowing temperathre was 25°C in 30s. Testing different cryoprotectant, adding 10% methanol to extender 1 gave the highest fertilization rate (64.6%) among cryoprotectant that used with this extender. In the other extender (2) adding 5% DMSO and mixture of 5% DMSO and 1% glycerol gave the highest fertilization rates (64.8% and 67.0%, respectively). In conclusion using extender 1 and 2 with mentioned cryoprotectant and thowing rate of 25°C in 30 s was recammended for rainbow trout sperm cryopreservation.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: Breeding has moved beyond the point of simply propagating random stock. Genetic potential can be improved by establishing and crossing genetically defined strains, crossing species and manipulating gametes. Some of these activities require cryopreservation of semen. Cryopreserved sperm could also be a means of exchange of genetic material between locations and populations when transportation of live fish is restricted. In this study we had a survey on rainbow trout sperm quality in Genetic and Breeding Center for Coldwatwr Fishes, Shahid Motahari, Yasouj. Then Cryopreservation trials on rainbow trout (Oncorhynchus mykiss) sperm were carried out using six basic extenders. Egg batches of 25 g were inseminated with semen frozen in five 0.5-ml straws. Thowing temperature of cryopreserved sperm also was tested. In anather trial we investigate different cryoprotectant. The result showd that the best period for sperm collection in this center was from Novamber to January. The best extender based on sperm motility parameters were extender 2 and 1 with 57.2 and 56.9% fertilisatin rates, respectively. So this two extender were used in the further experiments. The best thowing temperathre was 25°C in 30s. Testing different cryoprotectant, adding 10% methanol to extender 1 gave the highest fertilization rate (64.6%) among cryoprotectant that used with this extender. In the other extender (2) adding 5% DMSO and mixture of 5% DMSO and 1% glycerol gave the highest fertilization rates (64.8% and 67.0%, respectively). In conclusion using extender 1 and 2 with mentioned cryoprotectant and thowing rate of 25°C in 30 s was recammended for rainbow trout sperm cryopreservation.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: This study was determined some important extracted materials, Such as : Alginates of sodium & calcium and alginic acid in three species of brown seaweeds (Sargassum illicifolium , Cystoseira indica and Nizimuddinia zanardinii) during in different season in 1387 in coastal erea of Sistan and Baluochestan Provience. At First seaweeds sample were colected from sea beach and transferd to lab, so washed in fresh water and dried in the sun. So were under Alkaloid extraction with sodium carbonate (5 Persent), that were obtined fluid after filteration. The sodium alginate fibers extracted from fluid were combined by Alcohol. The calacium alginate fibers were deposited by calacium chlorids (5%) and Alginic acid resulted with chloridric acid (1N). The extraction yield of sodium alginate, calacium alginate and alginic acid in different samples of seaweeds with following Explaination: The presentage we have got, can be said , the present average of sodium alginate at Sargassum in Spring season in three areas (Chabahar, Tang and Pasabandar ) Respectively: 28.4±2 , 28.9±2 , 27.2±1.9 present, the present average of sodium alginate at Cystoseira in Spring in three areas at above mentioned goes in this way: 19.7±1.4, 18.6±1.4, 19.2±1.3 present and the present average of sodium alginate at Nizimuddinia in Spring were 23.4±1.5, 23.8±1.5 , 20.6±1.5 in these three areas. The present average of calacium alginate at Sargassum in Spring season in three areas (Chabahar, Tang and Pasabandar ): 33.7±0.5 , 33.7±0.8 , 33.1±1 present, the present average of calacium alginate at Cystoseira in Spring in three areas at above mentioned goes in this way: 28.8±1.5, 27.4±1, 27.4±1.5 present and the present average of calacium alginate at Nizimuddinia in Spring were 35.9±2.8, 23.9±1.5 , 35.9±2.8 in these three areas. The present average of Alginic acid at Sargassum in Spring season in three areas (Chabahar, Tang and Pasabandar ): 19.2±1.6 , 25.6±1.7 , 18.4±1.1 present, the present average of Alginic acid at Cystoseira in Spring in three areas at above mentioned goes in this way: 16.8±1.2, 15.8±1.1, 16.4±1 present and the present average of Alginic acid at Nizimuddinia in Spring were 19.2±1.6, 19.6±1.7 , 18.4±1.5 in these three areas. The present average of sodium alginate at Sargassum in Summer season in three areas (Chabahar, Tang and Pasabandar ) Respectively: 20.8±1 , 21±1 , 27.8±1.9 present, the present average of sodium alginate at Cystoseira in Summer in three areas at above mentioned goes in this way: 21±1.1, 13.2±1, 14.1±0.7 present and the present average of sodium alginate at Nizimuddinia in Summer were 25.3±2.3, 16.7±2.1 , 15.3±1.3 in these three areas. The present average of calacium alginate at Sargassum in Summer season in three areas (Chabahar, Tang and Pasabandar ): 29.5±2.1 , 29.7±2 , 28.3±2.2 present, the present average of calacium alginate at Cystoseira in Summer in three areas at above mentioned goes in this way: 21±1.1, 20.2±0.9, 20±0.7 present and the present average of calacium alginate at Nizimuddinia in Summer were 25.3±2.3, 29.7±2 , 23.1±1.5 in these three areas. The present average of Alginic acid at Sargassum in Summer season in three areas (Chabahar, Tang and Pasabandar ): 19.5±0.7 , 19.7±0.6 , 18.7±0.7 present, the present average of Alginic acid at Cystoseira in Summer in three areas at above mentioned goes in this way: 21±1.1, 11.5±1.4, 11.1±1.5 present and the present average of Alginic acid at Nizimuddinia in Summer were 14.8±1.3, 15.3±1.3 , 14.6±1 in these three areas. The present average of sodium alginate at Sargassum in Autumn season in three areas (Chabahar, Tang and Pasabandar ) Respectively: 31.5±4.3 , 31.6±4.8 , 29.4±4.8 present, the present average of sodium alginate at Cystoseira in Autumn in three areas at above mentioned goes in this way: 23±2.7, 21.4±2.8, 21.4±2.7 present and the present average of sodium alginate at Nizimuddinia in Autumn were 25.8±2.2, 26.4±2.1 , 23.4±1.9 in these three areas. The present average of calacium alginate at Sargassum in Autumn season in three areas (Chabahar, Tang and Pasabandar ): 43.3±1.6 , 42.8±2.7 , 41.7±2.9 present, the present average of calacium alginate at Cystoseira in Autumn in three areas at above mentioned goes in this way: 34.3±5.9, 32.4±5.5, 30.9±5.2 present and the present average of calacium alginate at Nizimuddinia in Autumn were 50±7.1, 50.2±7.5 , 45.6±5.2 in these three areas. The present average of Alginic acid at Sargassum in Autumn season in three areas (Chabahar, Tang and Pasabandar ): 28±3.8 , 28±3.5 , 26±3.9 present, the present average of Alginic acid at Cystoseira in Autumn in three areas at above mentioned goes in this way: 20.2±1.8, 19.2±2.1, 18.7±1.6 present and the present average of Alginic acid at Nizimuddinia in Autumn were 25.9±3, 26.1±2.9 , 24.6±3.6 in these three areas. The present average of sodium alginate at Sargassum in Winter season in three areas (Chabahar, Tang and Pasabandar ) Respectively: 35±1.6, 35.2±1.8 , 33.8±1.9 present, the present average of sodium alginate at Cystoseira in Winter in three areas at above mentioned goes in this way: 24.1±2, 22.9±1.2, 23.3±0.7 present and the present average of sodium alginate at Nizimuddinia in Winter were 28.2±1.3, 29.2±1.7 , 26.2±1.8 in these three areas. The present average of calacium alginate at Sargassum in Winter season in three areas (Chabahar, Tang and Pasabandar ): 45.6±1.9 , 45±2 , 44.6±2.5 present, the present average of calacium alginate at Cystoseira in Winter in three areas at above mentioned goes in this way: 38.2±2.8, 35.9±3.4, 35.6±3.5 present and the present average of calacium alginate at Nizimuddinia in Winter were 56.4±3.5, 56±3.5 , 51.8±2.5 in these three areas. The present average of Alginic acid at Sargassum in winter season in three areas (Chabahar, Tang and Pasabandar ): 31.8±1.2 , 32.1±0.9 , 30.9±1.2 present, the present average of Alginic acid at Cystoseira in Winter in three areas at above mentioned goes in this way: 21.1±1.2, 20.4±1.4, 19.3±1.4 present and the present average of Alginic acid at Nizimuddinia in Winter were 26.4±2.1, 27±2.2 , 24.6±2.2 in these three areas. The more amount of calacium alginate in Autumn can be found at Nizimuddinia in Tang area , wite amount of 50.2 present and the lates amount of Alginic acid at Cystoseira can be found in Summer with 11.1 present. If the Alginic acid become more than 20 present , it can be economical , that it can be showed more than 20 present in Autumn and Winter . According to this results, One way ANOVA showed that average of Alginates were not similar and significant differences ( P〈 0.05 ) between species.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: Growth parameters, mortality and exploitation rates were estimated from monthly lenght frequency samples (March 2002 to Feburary 2003) for .....

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: Due to approaching the method of marine algae (G. corticata) mariculture in the Chabahar waters (located at NE of Oman Sea), a series of feasibility studies were carried out to identify the suitable areas for growth of this algae. In this regard, different regions of shallow waters, intertidal zones, and also natural habitats of them were selected for either collecting sample or as culture regions. During the growth periods in autumn, winter and spring (the culture and plantation were not carried out during summer time synchoronized with monsoon season). Some acceptable results were achieved that indicated the meaningful growth rate of plants in the selected stations. According to the obtained results, at the end of culture period the weight growth was 3 times more and the mean length growth of C. coricata talls was from 68 to 134mm at the end of culture period. On the other hand, this statistical analysis didn't show any significant differences in different seasons from point of weight growth. It should be mentioned that some physical and chemical factors of sea water were measured consist of salinity, temperature, DO., pH, nitrate, phosphate and turbidity.

Description: Published

Description: Growth experiments were conducted in a random statistical design to determine the dietary requirements of Persian sturgeon, Acipenser persicus. In this study the effects of various dietary protein and energy and their optimum P/E proportion in diets was estimated on growth and carcass composition in A. persicus in the fingerling and grow out stage in three different phases. In the first phase, 16 semi pure diets with 4 protein levels (35, 40, 45 and 50%), each with four energy levels (18.5, 19.8, 21.1 and 22.4 megjoules/kg diet) and P/E proportion of 15.63 to 26.4 mg protein KJ-1 were formulated and fed to 960 A. persicus fingerlings, with a mean weight of 10.26±0.11 for 112 days. Three replicates were used for each experiment group. In the second phase, 432 A. persicus juveniles with a mean weight of 112.25±1.187 g were fed 12 semi purified diets with 3 protein levels (40, 45 and 50%) each with 4 energy levels (18.5, 19.8, 21.1 and 22.4 megjoules/kg diet) and P/E proportion of 18.9 to 26.4 mg protein KJ-1 for a period of 101 days using three replicates for each treatment. In the third phase, 384 juvenile A. persicus with a mean weight of 803.26±3.5 g were fed 16 diets for a period of 140 days. Two replicates were used for each treatment group. At the end of the experiment 30% of the fish population was randomly selected for carcass analysis to determine protein, lipid, moisture and ash content. Hepatosomatic index of the fish specimens was also determined. In the fingerling stage the fish fed 45 and 50% protein with 22.4 megjoules/kg diet energy exhibited the best growth indices [final body weight, percentage body weight increase (BWI%) and specific growth rate (SGR)] (P〈0.05). A diet containing 40% protein and 22.4 megjoules/kg diet energy with P/E ratio of 17.86 mg protein KJ-1 is the recommended diet for A. persicus in this stage. In the first phase higher growth rates were observed in fish fed diets containing 40% protein as compared to those fed diets containing 45 and 50% protein. Increasing energy levels to 21.1 and 22.4 megjoules/kg diet significantly increased (P〈0.05) growth indices. Highest values for growth indices belonged to fish fed diets containing 40% protein, 22.4 megjoules/kg diet energy and P/E ratios of 17.86 mg protein KJ-1. Final weight, BWI% and SGR in fish fed diets containing 40% protein were higher as compared to fish fed diets containing 35, 45 and 50% protein. Increasing energy levels to 22.4 megjoules/kg diet resulted in significant increase in growth indices as well as P/E ratios. Highest growth indices were recorded with diets containing 40% protein, 22.4 megjoules/kg diet energy and a P/E ratio of 17.86 mg protein KJ-1. Results obtained indicate that A. persicus showed better growth rates after acclimation to formulated diets, and therefore can be considered a suitable species for culture in captivity. On the basis of growth rate and carcass composition diet containing 40% protein, 20.1 to 25.9% lipid with a P/E ratio of 17.86 mg protein KJ-1 prepared from good quality fishmeal, fish oil and vegetable oil (sunflower or soybean oil) is recommended for feeding A. persicus in the fingerling and grow out stages.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: Research project was conducted on some biological aspects of lobster in Oman Sea in the years1385-86. During the project some parameters such as length distribution, spawning peak, length (Carapace) at first maturity, the length at which 50% of the lobsters were found to be mature sexually, sex ratio, relative frequency index and population dynamics parameters were examined. Quantitative factors and catch composition of lobster including the amount of catch in terms of weight and number of ovigerous-female, under-sized specimens and so were compared by monthly. According to the results of present study caught specimens were male with carapace length of 22 and 110 mm respectively. The smallest ovigerous female was taken as 46mm CL when length at first maturity was estimated to be 69-70mm. the male: female ratio was 0.7: 1.3 for the whole year. A closed fishing season for the species is proposed during spring and September and October in Oman Sea. A large amount of the catches for the all sampling months came from the under-sized specimens, consist of 50 to 85% of the total catch. Therefore, presence of these small length groups have direct effects on time of closing season. Population dynamics parameters were calculated as follows: K=0.57 year, L∞= 103mm and Ǿ = 8.69. Total mortality (Z), natural mortality (M) and fishing mortality (F) coefficients were calculated as 2.73, 0.87 and 1.86, respectively. Exploitation ratio for the whole population was 0.681, indicating an overexploitation of the species. Based on the available data, the recruitment pattern for lobster was obtained for two season including spring (major) and autumn (minor). Catch per unite effort (CPUE) was calculated as 0.908 kg/Cage/day which was higher in comparison with two other areas including Ramin and Chabahar. Examination of CPUE for a 10-year period showed a decreased trend and the reduction for the year 1386 was about 30% when compared to the data from the year 1375. Moreover, the mean length during 1369-70 to 1385-86 (present study) decreased for the three areas including Pozm, Ramin and Chabahar, so that the carapas length decreased up to 10 mm in length. This sudden decrease in mean length during the 10-year period should be considered as a serious precariousness for sustainable exploitation of lobster in Oman Sea along Sistan and Baluchistan province.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: The effect of essential fatty acids (EFA) and vitamin C-enriched Artemia nauplii on growth, survival, and resistance to temperature (high) stress in rainbow trout larvae reared in tanks were investigated. The larvae (average weight 120.43mg±13.5) were fed 6 times daily starting at the onset of exogenous feeding for 1 week. Triplicate groups of fish were offered one of four treatments (1) commercial starter food for rainbow trout، (2) newly hatched Artemia nauplii (unenriched) ،(3) highly unsaturated fatty acid (HUFA) + vitamin C-enriched Artemia nauplii and (4) combination of 10 % HUFA+ vitamin C enriched nauplii and commercial starter food. After 1 week, all groups of fish were switched to the commercial diet for an additional period of 3 weeks. Statistical analysis of growth after the first week and at the end of the experiment, showed that growth of larvae in various treatments was significantly different (P〈0.05). After 4 weeks, the larvae in treatment 3 with the average weight of 657.50 ± 57.93 mg had the highest body weight (P〈0.05). The highest percentage of survival (96%) was observed in treatment 3 (P〈0.05). Proximate compositions of trout larvae after one week feeding with experimental diets showed that the protein in the larvae of treatments 3 and 4 was significantly different compared to other treatments (P〈0.05). The best result of resistance to temperature (up to 24ºC) was observed in larvae reared on treatment 3 with 91.34 ± 1.52 percent (P〈0.05).

Description: Published