Publication Date:
2015-05-02
Description:
DNA interstrand cross-links (ICLs) block replication fork progression by inhibiting DNA strand separation. Repair of ICLs requires sequential incisions, translesion DNA synthesis, and homologous recombination, but the full set of factors involved in these transactions remains unknown. We devised a technique called chromatin mass spectrometry (CHROMASS) to study protein recruitment dynamics during perturbed DNA replication in Xenopus egg extracts. Using CHROMASS, we systematically monitored protein assembly and disassembly on ICL-containing chromatin. Among numerous prospective DNA repair factors, we identified SLF1 and SLF2, which form a complex with RAD18 and together define a pathway that suppresses genome instability by recruiting the SMC5/6 cohesion complex to DNA lesions. Our study provides a global analysis of an entire DNA repair pathway and reveals the mechanism of SMC5/6 relocalization to damaged DNA in vertebrate cells.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Raschle, Markus -- Smeenk, Godelieve -- Hansen, Rebecca K -- Temu, Tikira -- Oka, Yasuyoshi -- Hein, Marco Y -- Nagaraj, Nagarjuna -- Long, David T -- Walter, Johannes C -- Hofmann, Kay -- Storchova, Zuzana -- Cox, Jurgen -- Bekker-Jensen, Simon -- Mailand, Niels -- Mann, Matthias -- HL098316/HL/NHLBI NIH HHS/ -- Howard Hughes Medical Institute/ -- New York, N.Y. -- Science. 2015 May 1;348(6234):1253671. doi: 10.1126/science.1253671. Epub 2015 Apr 30.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Proteomics and Signal Transduction, Max Planck Institute of Biochemistry, 82152 Martinsried, Germany. ; Ubiquitin Signaling Group, Department of Disease Biology, Novo Nordisk Foundation Center for Protein Research, University of Copenhagen, DK-2200 Copenhagen, Denmark. ; Howard Hughes Medical Institute and Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA 02115, USA. ; Institute of Genetics, University of Cologne, 50674 Cologne, Germany. ; Maintenance of Genome Stability Group, Max Planck Institute of Biochemistry, 82152 Martinsried, Germany. ; Ubiquitin Signaling Group, Department of Disease Biology, Novo Nordisk Foundation Center for Protein Research, University of Copenhagen, DK-2200 Copenhagen, Denmark. simon.bekker-jensen@cpr.ku.dk niels.mailand@cpr.ku.dk mmann@biochem.mpg.de. ; Department of Proteomics and Signal Transduction, Max Planck Institute of Biochemistry, 82152 Martinsried, Germany. Novo Nordisk Foundation Center for Protein Research, Proteomics Program, University of Copenhagen, DK-2200 Copenhagen, Denmark. simon.bekker-jensen@cpr.ku.dk niels.mailand@cpr.ku.dk mmann@biochem.mpg.de.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/25931565" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Chromatin/chemistry/metabolism
;
*DNA Damage
;
*DNA Repair
;
DNA Repair Enzymes/*metabolism
;
*DNA Replication
;
DNA-Binding Proteins/metabolism
;
Mass Spectrometry/methods
;
Proteomics/methods
;
RNA-Binding Proteins/metabolism
;
Xenopus
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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