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  • 1
    Publication Date: 2018-04-19
    Description: A novel non-sequencing approach was developed to detect short DNA fragments ( ca 100 bp) for rapid authentication of two natural products, namely Testudinis Carapax et Plastrum and Trionycis Carapax, based on the difference in mitochondrial genome. Five specifically designed primer reactions were established to target species for reliable identification of their commercial products. They were confirmed to have a high level of inter-species-specificity and good intra-species stability. The limit of detection was estimated to be 1 ng of genomes for all of five assays. Also, the validation results demonstrated that the raw materials and processed products in addition to some of the highly processed products can be conveniently authenticated with good sensitivity and precision by this newly proposed approach. Especially, when reference sample mixtures were assayed, these primer sets have still performed well but not the prevailing COI barcoding technology. These could assist in the discrimination and identification of other animal-derived medicines for their form of raw material, the pulverized and the complex.
    Keywords: biochemistry, biotechnology
    Electronic ISSN: 2054-5703
    Topics: Natural Sciences in General
    Published by Royal Society
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  • 2
    Publication Date: 2016-04-07
    Description: We invert Global Positioning System (GPS) velocity data to estimate fault slip rates in California using a fault-based crustal deformation model with geologic constraints. The model assumes buried elastic dislocations across the region using Uniform California Earthquake Rupture Forecast Version 3 (UCERF3) fault geometries. New GPS velocity and geologic slip-rate data were compiled by the UCERF3 deformation working group. The result of least-squares inversion shows that the San Andreas fault slips at 19–22 mm/yr along Santa Cruz to the North Coast, 25–28 mm/yr along the central California creeping segment to the Carrizo Plain, 20–22 mm/yr along the Mojave, and 20–24 mm/yr along the Coachella to the Imperial Valley. Modeled slip rates are 7–16 mm/yr lower than the preferred geologic rates from the central California creeping section to the San Bernardino North section. For the Bartlett Springs section, fault slip rates of 7–9 mm/yr fall within the geologic bounds but are twice the preferred geologic rates. For the central and eastern Garlock, inverted slip rates of 7.5 and 4.9 mm/yr, respectively, match closely with the geologic rates. For the western Garlock, however, our result suggests a low slip rate of 1.7 mm/yr. Along the eastern California shear zone and southern Walker Lane, our model shows a cumulative slip rate of 6.2–6.9 mm/yr across its east–west transects, which is ~1 mm/yr increase of the geologic estimates. For the off-coast faults of central California, from Hosgri to San Gregorio, fault slips are modeled at 1–5 mm/yr, similar to the lower geologic bounds. For the off-fault deformation, the total moment rate amounts to 0.88 x 10 19 N·m/yr, with fast straining regions found around the Mendocino triple junction, Transverse Ranges and Garlock fault zones, Landers and Brawley seismic zones, and farther south. The overall California moment rate is 2.76 x 10 19 N·m/yr, which is a 16% increase compared with the UCERF2 model. Online Material: Table of geological slip rates.
    Print ISSN: 0037-1106
    Electronic ISSN: 1943-3573
    Topics: Geosciences , Physics
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  • 3
    Publication Date: 2016-04-15
    Description: We invert Global Positioning System (GPS) velocity data to estimate fault slip rates in California using a fault-based crustal deformation model with geologic constraints. The model assumes buried elastic dislocations across the region using Uniform California Earthquake Rupture Forecast Version 3 (UCERF3) fault geometries. New GPS velocity and geologic slip-rate data were compiled by the UCERF3 deformation working group. The result of least-squares inversion shows that the San Andreas fault slips at 19–22 mm/yr along Santa Cruz to the North Coast, 25–28 mm/yr along the central California creeping segment to the Carrizo Plain, 20–22 mm/yr along the Mojave, and 20–24 mm/yr along the Coachella to the Imperial Valley. Modeled slip rates are 7–16 mm/yr lower than the preferred geologic rates from the central California creeping section to the San Bernardino North section. For the Bartlett Springs section, fault slip rates of 7–9 mm/yr fall within the geologic bounds but are twice the preferred geologic rates. For the central and eastern Garlock, inverted slip rates of 7.5 and 4.9 mm/yr, respectively, match closely with the geologic rates. For the western Garlock, however, our result suggests a low slip rate of 1.7 mm/yr. Along the eastern California shear zone and southern Walker Lane, our model shows a cumulative slip rate of 6.2–6.9 mm/yr across its east–west transects, which is ~1 mm/yr increase of the geologic estimates. For the off-coast faults of central California, from Hosgri to San Gregorio, fault slips are modeled at 1–5 mm/yr, similar to the lower geologic bounds. For the off-fault deformation, the total moment rate amounts to 0.88 x 10 19 N·m/yr, with fast straining regions found around the Mendocino triple junction, Transverse Ranges and Garlock fault zones, Landers and Brawley seismic zones, and farther south. The overall California moment rate is 2.76 x 10 19 N·m/yr, which is a 16% increase compared with the UCERF2 model. Online Material: Table of geological slip rates.
    Print ISSN: 0037-1106
    Electronic ISSN: 1943-3573
    Topics: Geosciences , Physics
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  • 4
    Publication Date: 2015-09-11
    Print ISSN: 0895-0695
    Electronic ISSN: 1938-2057
    Topics: Geosciences
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  • 5
    Publication Date: 2017-11-16
    Description: The wetting of hollow fibre membranes decreases the performance of the liquid–gas membrane contactor for CO 2 capture in biogas upgrading. To solve this problem, in this work, a poly(vinylidene fluoride) (PVDF) hollow fibre membrane for a liquid–gas membrane contactor was coated with a superhydrophobic layer composed of a combination of hydrophobic SiO 2 nanoparticles and polydimethylsiloxane (PDMS) by the method of spray deposition. A rough layer of SiO 2 deposited on the PVDF membrane resulted in an enhanced surface hydrophobicity. The surface structure of the pristine PVDF significantly affected the homogeneity of the generated SiO 2 layer. A uniform surface coating on the PVDF upper layer resulted from the presence of micrometre and nanometre-sized roughness on the surface of the PVDF membrane, which was achieved with a SiO 2 concentration of 4.44 mg ml –1 (0.2 g/45 ml) in the coating solution. As a result, the water contact angle of the modified surface was recorded as 155 ± 3°, which is higher than that of the pristine surface. The high contact angle is advantageous for reducing the wetting of the membrane. Additional mass transfer resistance was introduced by the superhydrophobic layer. In addition, continuous CO 2 absorption tests were carried out in original and modified PVDF hollow fibre membrane contactors, using monoethanolamine (MEA) solution as the absorbent. A long-term stability test revealed that the modified PVDF hollow fibre membrane contactor was able to outperform the original membrane contactor and demonstrated outstanding long-term stability, suggesting that spray deposition is a promising approach to obtain superhydrophobic PVDF membranes for liquid–gas membrane absorption.
    Keywords: energy
    Electronic ISSN: 2054-5703
    Topics: Natural Sciences in General
    Published by Royal Society
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  • 6
    Publication Date: 2018-09-27
    Description: Transparent tubes with functions of heating and temperature measurement are badly required in the visualization investigation of two-phase flows and flow-boiling heat transfer. In order to prepare such a tube, we introduced a cost-effective and energy-efficient procedure of hypergravity-assisted chemical liquid deposition (HACLD) to produce transparent and conductive silver (Ag) films on the inner surfaces of quartz tubes, typically 50 mm in length and 8 mm in inner diameter with a set-up that was designed and built for this purpose. Precursors of organometallic Ag precursor solutions were prepared by dissolving silver citrate and 1,2-diaminopropane in 2-methoxyethanol with required concentration for the chemical liquid deposition process. Semitransparent and conductive Ag films formed inside the required quartz tubes under specific heating process in hypergravity. One of the films was about 47 nm in thickness, 23 per square sheet resistance, and 30% optical transmittance. This attempt may pave a way for the understanding of the film forming mechanism in hypergravity, and the development of a film preparation technology of HACLD.
    Keywords: chemical physics
    Electronic ISSN: 2054-5703
    Topics: Natural Sciences in General
    Published by Royal Society
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