ALBERT

Description: The Red Sea is an extreme marine environment, with conditions limiting the application of standard geochemical proxies for the reconstruction of paleoclimate. In order to develop paleoenvironmental reconstruction methods which are not dependent on chemical signals, we investigated the distribution of planktonic foraminifera in the surface sediments and assessed the viability of constructing foraminiferal transfer functions in this basin. We find a distinct gradient in the faunal assemblage along the basin's axis, which is reflected in a high correlation between faunal composition and all considered environmental parameters (temperature, salinity, chlorophyll a concentration, stratification, and oxycline depth). As a result, transfer functions constructed by different methods (ANN, MAT, IKM, WA-PLS) appear to be able to estimate all of these parameters with a high average accuracy (15% of the parameter's range in the Red Sea). However, redundancy analysis of the distribution of foraminiferal assemblages in surface sediments alone did not yield unambiguous results in terms of which of the considered factors exerts a primary control on the foraminifera distribution and which of the observed relationships are the result of the mutual correlation among the environmental factors. To disentangle the effect of individual environmental parameters, we applied the obtained transfer functions on a newly generated Holocene record from the central Red Sea. The integration of published paleoclimate reconstructions with our data allowed us to identify productivity as the most likely primary control of the planktonic foraminifera distribution in the Red Sea. The generated transfer functions can estimate paleoproductivity with acceptable accuracy (RMSEP chlorophyll a = 0.1 mg/m**3; ~ 8% of recent range), but only under such conditions in the past when circulation patterns and salinity levels in the basin were fundamentally comparable to the present day. Since productivity in the central and southern Red Sea is closely linked with the Monsoon-driven water exchange across the Strait of Bab al Mandab, the resulting reconstructions can provide indirect information on the mode and intensity of the monsoonal system in the past.

Description: Shallow marine ecosystems naturally experience fluctuating physicochemical conditions across spatial and temporal scales. Widespread coral-bleaching events, induced by prolonged heat stress, highlight the importance of how the duration and frequency of thermal stress influence the adaptive physiology of photosymbiotic calcifiers. Large benthic foraminifera harboring algal endosymbionts are major tropical carbonate producers and bioindicators of ecosystem health. Like corals, they are sensitive to thermal stress and bleach at temperatures temporarily occurring in their natural habitat and projected to happen more frequently. However, their thermal tolerance has been studied so far only by chronic exposure, so how they respond under more realistic episodic heat-event scenarios remains unknown. Here, we determined the physiological responses of Amphistegina gibbosa, an abundant western Atlantic foraminifera, to four different treatments--control, single, episodic, and chronic exposure to the same thermal stress (32°C)--in controlled laboratory cultures. Exposure to chronic thermal stress reduced motility and growth, while antioxidant capacity was elevated, and photosymbiont variables (coloration, oxygen-production rates, chlorophyll a concentration) indicated extensive bleaching. In contrast, single- and episodic-stress treatments were associated with higher motility and growth, while photosymbiont variables remained stable. The effects of single and episodic heat events were similar, except for the presumable occurrence of reproduction, which seemed to be suppressed by both episodic and chronic stress. The otherwise different responses between treatments with thermal fluctuations and chronic stress indicate adaptation to thermal peaks, but not to chronic exposure expected to ensue when baseline temperatures are elevated by climate change. This firstly implies that marine habitats with a history of fluctuating thermal stress potentially support resilient physiological mechanisms among photosymbiotic organisms. Secondly, there seem to be temporal constraints related to heat events among coral reef environments and reinforces the importance of temporal fluctuations in stress exposure in global-change studies and projections.

Description: Shell fluxes of planktonic Foraminifera species vary intra-annually in a pattern that appears to follow the seasonal cycle. However, the variation in the timing and prominence of seasonal flux maxima in space and among species remains poorly constrained. Thus, although changing seasonality may result in a flux-weighted temperature offset of more than 5° C within a species, this effect is often ignored in the interpretation of Foraminifera-based paleoceanographic records. To address this issue we present an analysis of the intra-annual pattern of shell flux variability in 37 globally distributed time series. The existence of a seasonal component in flux variability was objectively characterised using periodic regression. This analysis yielded estimates of the number, timing and prominence of seasonal flux maxima. Over 80% of the flux series across all species showed a statistically significant periodic component, indicating that a considerable part of the intra-annual flux variability is predictable. Temperature appears to be a powerful predictor of flux seasonality, but its effect differs among species. Three different modes of seasonality are distinguishable. Tropical and subtropical species (Globigerinoides ruber (white and pink varieties), Neogloboquadrina dutertrei, Globigerinoides sacculifer, Orbulina universa, Globigerinella siphonifera, Pulleniatina obliquiloculata, Globorotalia menardii, Globoturborotalita rubescens, Globoturborotalita tenella and Globigerinoides conglobatus) appear to have a less predictable flux pattern, with random peak timing in warm waters. In colder waters, seasonality is more prevalent: peak fluxes occur shortly after summer temperature maxima and peak prominence increases. This tendency is stronger in species with a narrower temperature range, implying that warm-adapted species find it increasingly difficult to reproduce outside their optimum temperature range and that, with decreasing mean temperature, their flux is progressively more focussed in the warm season. The second group includes the temperate to cold-water species Globigerina bulloides, Globigerinita glutinata, Turborotalita quinqueloba, Neogloboquadrina incompta, Neogloboquadrina pachyderma, Globorotalia scitula, Globigerinella calida, Globigerina falconensis, Globorotalia theyeri and Globigerinita uvula. These species show a highly predictable seasonal pattern, with one to two peaks a year, which occur earlier in warmer waters. Peak prominence in this group is independent of temperature. The earlier-when-warmer pattern in this group is related to the timing of productivity maxima. Finally, the deep-dwelling Globorotalia truncatulinoides and Globorotalia inflata show a regular and pronounced peak in winter and spring. The remarkably low flux outside the main pulse may indicate a long reproductive cycle of these species. Overall, our analysis indicates that the seasonality of planktonic Foraminifera shell flux is predictable and reveals the existence of distinct modes of phenology among species. We evaluate the effect of changing seasonality on paleoceanographic reconstructions and find that, irrespective of the seasonality mode, the actual magnitude of environmental change will be underestimated. The observed constraints on flux seasonality can serve as the basis for predictive modelling of flux pattern. As long as the diversity of species seasonality is accounted for in such models, the results can be used to improve reconstructions of the magnitude of environmental change in paleoceanographic records.

Description: The proliferation of key marine ecological engineers and carbonate producers often relies on their association with photosymbiotic algae. Evaluating stress responses of these organisms is important to predict their fate under future climate projections. Physiological approaches are limited in their ability to resolve the involved molecular mechanisms and attribute stress effects to the host or symbiont, while probing and partitioning of proteins cannot be applied in organisms where the host and symbiont are small and cannot be physically separated. Here we apply a label-free quantitative proteomics approach to detect changes of proteome composition in the diatom-bearing benthic foraminifera Amphistegina gibbosa experimentally exposed to three thermal-stress scenarios. We developed a workflow for protein extraction from less than ten specimens and simultaneously analysed host and symbiont proteomes. Despite little genomic data for the host, 1,618 proteins could be partially assembled and assigned. The proteomes revealed identical pattern of stress response among stress scenarios as that indicated by physiological measurements, but allowed identification of compartment-specific stress reactions. In the symbiont, stress-response and proteolysis-related proteins were up regulated while photosynthesis-related proteins declined. In contrast, host homeostasis was maintained through chaperone up-regulation associated with elevated proteosynthesis and proteolysis, and the host metabolism shifted to heterotrophy.

Description: We compared the responses of large benthic foraminifera to thermal stress in specimens from a population of Amphistegina lessonii, an abundant Indo-Pacific species, to specimens of A. gibbosa, its Atlantic counterpart, from a similar environment but two different water depths (5 m and 18 m). The test groups were exposed in a common experiment to three thermal-stress scenarios over a four-week period: (a) no thermal stress, i.e., control conditions at constant 25.5°C; (b) a single thermal stress event up to 32°C for three days, followed by control conditions; (c) episodic thermal stress events alternating with periods of six days at control conditions, and (d) chronic thermal stress at 32°C. Growth, respiration, mortality, and motility were measured to characterize the holobiont response. Coloration, photosynthesis, and chlorophyll a content were measured to determine the response of the endosymbiotic diatoms.