Publikationsdatum:
2018-01-30
Beschreibung:
Sensors, Vol. 18, Pages 385: A New Strategy Involving the Use of Peptides and Graphene Oxide for Fluorescence Turn-on Detection of Proteins Sensors doi: 10.3390/s18020385 Authors: Huan Shi Bibo Zhang Shuwen Liu Chunyan Tan Ying Tan Yuyang Jiang The detection of proteins is of great biological significance as disease biomarkers in early diagnosis, prognosis tracking and therapeutic evaluation. Thus, we developed a simple, sensitive and universal protein-sensing platform based on peptide and graphene oxide (GO). The design consists of a fluorophore (TAMRA, TAM), a peptide containing eight arginines and peptide ligand that could recognize the target protein, and GO used as a quencher. To demonstrate the feasible use of the sensor for target detection, Bcl-xL was evaluated as the model target. The sensor was proved to be sensitive and applied for the detection of the target proteins in buffer, 2% serum and living cells.
Digitale ISSN:
1424-8220
Thema:
Chemie und Pharmazie
,
Elektrotechnik, Elektronik, Nachrichtentechnik
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