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Photocage-initiated time-resolved solution X-ray scattering investigation of protein dimerization (2018)

Josts, I.Niebling, S.Gao, Y.Levantino, M.Tidow, H.Monteiro, D.

International Union of Crystallography (IUCr)

In: IUCrJ

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Publication Date: 2018-09-13

Description: This work demonstrates a new method for investigating time-resolved structural changes in protein conformation and oligomerization via photocage-initiated time-resolved X-ray solution scattering by observing the ATP-driven dimerization of the MsbA nucleotide-binding domain. Photocaged small molecules allow the observation of single-turnover reactions of non-naturally photoactivatable proteins. The kinetics of the reaction can be derived from changes in X-ray scattering associated with ATP-binding and subsequent dimerization. This method can be expanded to any small-molecule-driven protein reaction with conformational changes traceable by X-ray scattering where the small molecule can be photocaged.

Keywords: biophysicsX-ray solution scatteringphotocagingstructural biology

Electronic ISSN: 2052-2525

Topics: Geosciences , Physics

Published by International Union of Crystallography (IUCr)

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