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  • Books
  • Articles  (8)
  • Blackwell Publishing Ltd  (5)
  • Molecular Diversity Preservation International  (3)
  • 2015-2019  (3)
  • 1985-1989  (5)
  • Biology  (7)
  • Computer Science  (1)
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  • Books
  • Articles  (8)
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  • 1
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    Actinobaculum massiliense Proteome Profiled in Polymicrobial Urethral Catheter Biofilms (2018)
    Molecular Diversity Preservation International
    Details
    Publication Date: 2018-12-09
    Description: Actinobaculum massiliense, a Gram-positive anaerobic coccoid rod colonizing the human urinary tract, belongs to the taxonomic class of Actinobacteria. We identified A. massiliense as a cohabitant of urethral catheter biofilms (CB). The CBs also harbored more common uropathogens, such as Proteus mirabilis and Aerococcus urinae, supporting the notion that A. massiliense is adapted to a life style in polymicrobial biofilms. We isolated a clinical strain from a blood agar colony and used 16S rRNA gene sequencing and shotgun proteomics to confirm its identity as A. massiliense. We characterized this species by quantitatively comparing the bacterial proteome derived from in vitro growth with that of four clinical samples. The functional relevance of proteins with emphasis on nutrient import and the response to hostile host conditions, showing evidence of neutrophil infiltration, was analyzed. Two putative subtilisin-like proteases and a heme/oligopeptide transporter were abundant in vivo and are likely important for survival and fitness in the biofilm. Proteins facilitating uptake of xylose/glucuronate and oligopeptides, also highly expressed in vivo, may feed metabolites into mixed acid fermentation and peptidolysis pathways, respectively, to generate energy. A polyketide synthase predicted to generate a secondary metabolite that interacts with either the human host or co-colonizing microbes was also identified. The product of the PKS enzyme may contribute to A. massiliense fitness and persistence in the CBs.
    Electronic ISSN: 2227-7382
    Topics: Biology , Chemistry and Pharmacology
    Published by Molecular Diversity Preservation International
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  • 2
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    Better Understanding the Potential Importance of Florida Roadside Breeding Habitat for the Monarch (2018)
    Molecular Diversity Preservation International
    Details
    Publication Date: 2018-10-11
    Description: The North American monarch butterfly (Danaus plexippus) population has declined significantly over the past two decades. Among the many other factors, loss of breeding habitat has been implicated as a potential leading driver. In response, wildlife agencies and conservation practitioners have made a strong push to restore and conserve milkweeds on both wild and managed landscapes including agricultural lands as well as transportation and utility rights-of-way. Roadsides in particular have been emphasized as a targeted landscape for monarch habitat restoration. While much attention has been focused on habitat in California, along the I-35 corridor from Texas to Minnesota, and more broadly across the agricultural Midwest, research on the occurrence of roadside breeding habitat and the development of best vegetative management practices conducted in the Deep South has been limited. We sampled roadside verges in north-central Florida for the presence of two early season milkweed species, that are particularly important for early season monarch recolonization, Asclepias tuberosa and Asclepias humistrata. Our findings suggest that roadsides harbor extensive populations of the target milkweeds with the vast majority of plants occurring on the back slope of the verge. Alterations to current roadside mowing frequency and scope are needed to effectively conserve these populations and ensure that they are available for use by the monarch.
    Electronic ISSN: 2075-4450
    Topics: Biology
    Published by Molecular Diversity Preservation International
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  • 3
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    Microplastic Contamination Has Limited Effects on Coral Fertilisation and Larvae (2019)
    Molecular Diversity Preservation International
    Details
    Publication Date: 2019-11-28
    Description: Microplastics are ubiquitous throughout the world’s oceans and contaminate coral reef ecosystems. There is evidence of microplastic ingestion by corals and passive contact with coral tissues, causing adverse health effects that include energy expenditure for particle removal from the tissue surface, as well as reduced growth, tissue bleaching, and necrosis. Here, it was examined whether microplastic contamination impairs the success of gamete fertilisation, embryo development and larval settlement of the reef-building coral Acropora tenuis. Coral gametes and larvae were exposed to fifteen microplastic treatments using two types of plastic: (1) weathered polypropylene particles and (2) spherical polyethylene microbeads. The treatments ranged from five to 50 polypropylene pieces L−1 and 25 to 200 microbeads L−1. Fertilisation was only negatively affected by the largest weathered microplastics (2 mm2), but the effects were not dose dependent. Embryo development and larval settlement were not significantly impacted by either microplastic type. The study shows that moderate–high levels of marine microplastic contamination, specifically particles
    Electronic ISSN: 1424-2818
    Topics: Biology
    Published by Molecular Diversity Preservation International
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  • 4
    Electronic Resource
    Electronic Resource
    Learning and discovery: one system's search for mathematical knowledge (1988)
    Oxford, UK : Blackwell Publishing Ltd
    Computational intelligence 4 (1988), S. 0 
    Details
    ISSN: 1467-8640
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Computer Science
    Notes: The Graph Theorist, GT, is a system that performs mathematical research in graph theory. From the definitions in its input knowledge base, GT constructs examples of mathematical concepts, conjectures and proves mathematical theorems about concepts, and discovers new concepts. Discovery is driven both by examples and by definitional form. The discovery processes construct a semantic net that links all of GT's concepts together.Each definition is an algebraic expression whose semantic interpretation is a stylized algorithm to generate a class of graphs correctly and completely. From a knowledge base of these concept definitions, GT is able to conjecture and prove such theorems as “The set of acyclic, connected graphs is precisely the set of trees” and “There is no odd-regular graph on an odd number of vertices.” GT explores new concepts either to develop an area of knowledge or to link a newly acquired concept into a pre-existing knowledge base. New concepts arise from the specialization of an existing concept, the generalization of an existing concept, and the merger of two or more existing concepts. From an initial knowledge base containing only the definition of “graph,” GT discovers such concepts as acyclic graphs, connected graphs, and bipartite graphs.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1467-8640.1988.tb00089.x
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  • 5
    Electronic Resource
    Electronic Resource
    Na-Ca interactions in barley seedlings: relationship to ion transport and growth (1989)
    Oxford, UK : Blackwell Publishing Ltd
    Plant, cell & environment 12 (1989), S. 0 
    Details
    ISSN: 1365-3040
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract Salt-stressed plants often show Ca deficiency symptoms. The effects of NaCl salinity (1 to 150 mol m-3) and supplemental Ca (10 mol m-3) on Na and Ca transport in barley (Hordeum vulgare L.) and their relationship to growth were investigated. The adjustment of Na and Ca transport was investigated by examining young seedlings exposed to short-term (immediate) and long-term (7 d) exposure to salinity. When the plants were exposed to long-term treatments of salinity, the rate of sodium accumulation in roots was approximately 10 to 15% of short-term treatments. No significant adjustment in the transport to the shoot was observed. Rates of tracer (22Na) transport were compared to calculated rates based on relative growth rates and tissue element concentrations. Comparisons between measured tracer and calculated rates of transport indicate that 22Na transport may underestimate transport to the shoot because of dilution of the tracer in the root cytoplasm. Calcium uptake showed only minor adjustment with time. Measured rates of tracer transport to the shoot correlated well with calculated values. The transport and tissue concentrations of Na were significantly affected by supplemental Ca. Calcium transport and tissue concentrations were markedly inhibited by salinity. Supplemental Ca increased Ca transport and accumulation at all NaCl treatments above that of control plants without supplemental Ca. Salinity inhibited plant growth at 150 mol m -3NaCl, but not at 75 mol m-3.Supplemental Ca significantly improved root length but not fresh weight after 7d of salinity, although differences in fresh weight were detected after 9d. There were significant Na-Ca interactions with ion transport, ion accumulation, and growth. The effects of salinity on Na and Ca transport to the shoot do not appear to play a major role in shoot growth of barley.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-3040.1989.tb02128.x
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  • 6
    Electronic Resource
    Electronic Resource
    Resistance of fully imbibed tomato seeds to very high salinities (1986)
    Oxford, UK : Blackwell Publishing Ltd
    Plant, cell & environment 9 (1986), S. 0 
    Details
    ISSN: 1365-3040
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract Seeds of Lyeopersicon esculentum cv. VF36 (a salt-sensitive cultivar), L. esculentum var. Edkawi (which is fairly salt-resistant), and a wild relative, L. cheesmanii, were exposed to various concentrations of NaCl, up to 460 mol m−3, either directly or following imbibition in non-saline nutrient solution. After 10 d exposure to salt, they were transferred to non-saline solution. All taxa showed some germination at the lowest salinity tested, 92 mol m−3 NaCl, but virtually no germination occurred at 184 mol m−3 NaCl or higher salinities. Within 2 d after removal of the salt stress, however, the seeds of L. esculentum reached control levels of germination, even if they had already been on the verge of germination when the stress was imposed. The seeds of L. cheesmanii were less resistant to NaCl. The physiological basis for the resistance of VF36 seeds is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-3040.1986.tb01625.x
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  • 7
    Electronic Resource
    Electronic Resource
    Comparison of movement and metabolism of indole-3-acetic acid and indole-3-butyric acid in mung bean cuttings (1988)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 74 (1988), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Indole-3-butyric acid (IBA) was much more effective than indole-3-acetic acid (IAA) in inducing adventitious root formation in mung bean (Vigna radiata L.) cuttings. Prolonging the duration of treatment with both auxins from 24 to 96 h significantly increased the number of roots formed. Labelled IAA and IBA applied to the basal cut surface of the cuttings were transported acropetally. With both auxins, most radioactivity was detected in the hypocotyl, where roots were formed, but relatively more IBA was found in the upper sections of the cuttings. The rate of metabolism of IAA and IBA in these cuttings was similar. Both auxins were metabolized very rapidly and 24 h after application only a small fraction of the radioactivity corresponded to the free auxins. Hydrolysis with 7 M NaOH indicates that conjugation is the major pathway of IAA and IBA metabolism in mung bean tissues. The major conjugate of IAA was identified tentatively as indole-3-acetylaspartic acid, whereas IBA formed at least two major conjugates. The data indicate that the higher root-promoting activity of IBA was not due to a different transport pattern and/or a different rate of conjugation. It is suggested that the IBA conjugates may be a better source of free auxin than those of IAA and this may explain the higher activity of IBA.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1988.tb02018.x
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  • 8
    Electronic Resource
    Electronic Resource
    Osmoregulation by potassium transport in Escherichia coli (1986)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 39 (1986), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract Cell turgor pressure determines the extent of K+ accumulation by Escherichia coli cells. K+ influx is mediated both by a constitutive system with a low affinity for K+ (Trk) and by an inducible high affinity system (Kdp). K+ efflux is controlled by as yet unidentified but independent systems. Cell K+ concentration may be the link between growth in media of high osmolarity and the concomitant accumulation of compatible solutes such as betaine.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1986.tb01845.x
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