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  • Articles  (49)
  • Wiley  (26)
  • Springer  (23)
  • American Geophysical Union (AGU)
  • American Meteorological Society
  • 2015-2019  (24)
  • 1985-1989  (25)
  • Medicine  (49)
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  • Articles  (49)
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  • 1
    Electronic Resource
    Electronic Resource
    Treatment of solids and petroleum hydrocarbons in storm runoff with an on-site detention basin (1986)
    Springer
    Bulletin of environmental contamination and toxicology 36 (1986), S. 548-555 
    Details
    ISSN: 1432-0800
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01623550
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  • 2
    Electronic Resource
    Electronic Resource
    Residues of chlorinated phenols and phenoxy acid herbicides in the urine of Arkansas children (1989)
    Springer
    Archives of environmental contamination and toxicology 18 (1989), S. 469-474 
    Details
    ISSN: 1432-0703
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Medicine
    Notes: Abstract Urine samples from 197 Arkansas children were analyzed for eight chlorinated phenols and four chlorinated phenoxy herbicides by using a new procedure that combined gas chromatography with tandem mass spectrometry. With the detection limit of 1 part per billion (ppb), six of these pesticides were detected in more than 10% of the samples. 2,5-Dichlorophenol (a metabolite ofp-dichlorobenzene), and pentachlorophenol were detected in 96% and 100%, respectively, of the children's urine at median concentrations of 9 ppb and 14 ppb, respectively. 2,4,5-Trichlorophenol was detected in 54% of the children's urine at a median concentration of 1 ppb. One trichlorophenol and three other dichlorophenols were found in 3% to 27% of the samples. The herbicide 2,4-dichlorophenoxyacetic acid was observed in 20% of all samples. The concentrations of all analytes are reported as background or reference levels for use in future studies. The finding of 2,5-dichlorophenol as a ubiquitous contaminant merits further study.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01055011
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  • 3
    Electronic Resource
    Electronic Resource
    An improved method for the isolation of rat cardiac sarcoplasmic reticulum (1988)
    Springer
    Molecular and cellular biochemistry 84 (1988), S. 87-95 
    Details
    ISSN: 1573-4919
    Keywords: rat ; cardiac sarcoplasmic reticulum ; Ca2+-ATPase ; calsequestrin ; 53 kD
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Summary Preparations of cardiac sarcoplasmic reticulum (CSR) isolated from the rat by differential centrifugation have been widely used for measuring alterations in intracellular calcium flux in response to metabolic and pharmacologic disruptions. However, the purity of these SR fractions has not been firmly established. Using a combination of differential and linear sucrose gradient centrifugation, we have isolated rat CSR with high specific activity and purity. By SDS-PAGE analysis, the preparation is enriched in a protein (110 kD) of similar size to the Ca2+-ATPase of SR from other sources. Gels stained with the dye ‘Stains All’ reveal a blue colored 55 kD band, confirming the presence of calsequestrin, the intraluminal low-affinity calcium binding protein of SR. The presence of the transmembrane 53 kD glycoprotein of SR was confirmed by endoglycosidase-H treatment followed by SDS-PAGE and also by a modified Western blotting technique. The rate of calcium uptake in this preparation averages 130 nmol/mg over the first minute of accumulation, approximately 4 times that previously reported for rat CSR. Calcium uptake in our preparation was essentially complete within 5 minutes. Preparations isolated by this method should be of value in future studies measuring alterations in rat CSR function.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00235196
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  • 4
    Electronic Resource
    Electronic Resource
    Influence of exercise on cardiac and skeletal muscle myofibrillar proteins (1988)
    Springer
    Molecular and cellular biochemistry 83 (1988), S. 27-36 
    Details
    ISSN: 1573-4919
    Keywords: fatigue ; myofibril ATPase ; Ca2+ regulation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract The purpose of this study was to examine the Ca2+-Mg2+ myofibrillar ATPase and protein composition of cardiac and skeletal muscle following strenuous activity to voluntary exhaustion. Sprague-Dawley rats (200 g) were assigned to a control and exercised group, with the run group completing 25 m·min−1 and 8% grade for 1 hour. Following activity, the myocardial Ca2+−Mg2+ myofibrillar ATPase activity -pCa relationship had undergone a rightward shift in the curve. Electrophoretic analysis revealed a change in the pattern of cardiac myofibrillar protein bands, particularly in the 38–42 Kdalton region. Enzymatic analysis of myofibrillar proteins from plantaris muscle, revealed no change in Ca2+ regulation following exercise. Electronmicrographic and electrophoretic analysis revealed extensively disrupted sarcomeric structure and a change in the ratio of several plantaris myofibrillar proteins. No difference was observed for myosin: Actin: tropomyosin ratios; however a dramatic reduction in 58 and 95 Kdalton proteins were evident. The results indicate that prolonged running is associated with similar responses in cardiac and skeletal muscle myofibrillar protein compositions. The abnormalities in myofibrillar ultrastructure may implicate force transmission failure as a factor in exercised-induced muscle damage and/or fatigue.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00223195
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  • 5
    Electronic Resource
    Electronic Resource
    Photoaffinity labeling of the DDT1 MF-2 cell α1-adrenergic receptor (1985)
    Springer
    Molecular and cellular biochemistry 67 (1985), S. 47-53 
    Details
    ISSN: 1573-4919
    Keywords: α1-adrenergic receptor ; membrane ; photoaffinity ; smooth muscle
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Summary In this study, we have used an α1-adrenergic receptor photoaffinity ligand, 2-[4-(4-azido-3-iodo-benzoyl)-piperazin-1-yl]-4-amino-6, 7-dimethoxyquinazoline (125I-APD), to label covalently the α1-adrenergic receptor in a smooth muscle cell line. Our results indicate that in the absence of light, (125I)APD binds reversibly to a site in the DDT1 MF-2 cell membranes having pharmacological characteristics of an α1-adrenergic receptor. Following incorporation of (125I)ADP into partially purified membranes a single labeled band of protein with a Mr of 81 000 was visualized by autoradiography following sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Incorporation of (125I)-APD into this band was affected by adrenergic agonists and antagonists in a manner consistent with an α1-adrenergic interaction. Prazosin (α1-selective) blocked incorporation of the label into the Mr = 81 000 protein while yohimbine (α2-selective) did not. Of the adrenergic agonists, (−)-epinephrine and (−)-norepinephrine but not (−)-isoproterenol blocked labeling of the Mr − 81 000 protein. We conclude that the ligand binding site of the DDT1 MF-2 cell α1-adrenergic receptor resides in a Mr = 81 000 protein.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00220985
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  • 6
    Electronic Resource
    Electronic Resource
    Glucocorticoids induce a 29 000 Mr protein in DDT1 MF-2 smooth muscle cells but not in the DDT1 MF-2 GR glucocorticoid resistant variant (1985)
    Springer
    Molecular and cellular biochemistry 68 (1985), S. 79-85 
    Details
    ISSN: 1573-4919
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract We have demonstrated that glucocorticoids induce in DDT1 MF-2 cells by a glucocorticoid mediated mechanism the synthesis of a methionine-cysteine rich protein of 29 000 Mr (p29). Induction of p29 is not observed in DDT1 MF-2 GR glucocorticoid resistant variants which have only 7% of glucocorticoid receptor site per cell compared to wild type cells. Increased synthesis of p29 is specific to glucocorticoids since neither androgens, estrogens, progesterone nor the glucocorticoid antagonist dexamethasone mesylate are effective inducers. Stimulation of p29 synthesis in wild type cells is observed at 10−10 M triamcinolone acetonide, reaching a maximum at a concentration of 1 × 10−8 M. The induction of p29 is not a function of glucocorticoid arrest of DDT1 MF-2 cells since DDT1 MF-2 cells promoted to re-enter the cell cycle by 50 ng/ml platelet derived growth factor (PDGF) continue synthesis of p29. Finally, increased levels of p29 translation products are observed in cell free translation assays carried out utilizing poly A− RNA transcripts isolated from glucocorticoid treated cells. These data suggest that the glucocorticoid stimulation of p29 synthesis is a transcriptional and/or RNA processing event controlled by glucocorticoid receptor complexes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00219391
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  • 7
    Electronic Resource
    Electronic Resource
    Glucocorticoid induction of β-adrenergic receptors in the DDT1 MF-2 smooth muscle cell line involves synthesis of new receptor (1987)
    Springer
    Molecular and cellular biochemistry 74 (1987), S. 21-27 
    Details
    ISSN: 1573-4919
    Keywords: glucocorticoid receptor ; β-adrenergic receptor regulation ; smooth muscle ; adenylate cyclase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract We have shown that glucocorticoids induce the appearance of β2-adrenergic receptors in membranes of the ductus deferens smooth muscle cell line (DDT1 MF-2). A concomitant increase in isoproterenol stimulated adenylate cyclase activity in the absence of exogenously applied GTP was observed as was a significantly increased (p 〈 0.05) sensitivity of the adenylate cyclase system to exogenously applied GTP. However, no significant difference in the maximal velocity of adenylate cyclase between control and steroid treatment was measurable in the presence of sodium fluoride. Induction of β2-adrenergic receptors in DDT1 MF-2 cells is correlated with the presence of steroid receptors (androgen and glucocorticoid) in the cells since estrogens and progesterones had no effect on receptor levels. Finally, utilizing dense amino acid labeling of cells to measure old versus newly synthesized receptor sites by a density shift method, we have documented that glucocorticoid induction of β2-adrenergic receptors involves synthesis of new receptor protein.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00221909
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  • 8
    Electronic Resource
    Electronic Resource
    A new parental cell line for human x human hybridoma production (1988)
    Springer
    Cytotechnology 1 (1988), S. 359-363 
    Details
    ISSN: 1573-0778
    Keywords: anti-idiotype ; B-chronic lymphocytic leukaemia ; HAT sensitive mutant ; human hybridoma ; lymphoblastoid cell line
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract The potential of a new HAT-sensitive human lymphoblastoid cell line TK6 TGr.P1. as a fusion partner was assessed, by comparison with the established human parental cell line UC729.6. Both of these cell lines were fused with the peripheral blood mononuclear cells of a patient with B-chronic lymphocytic leukaemia. The hybridomas generated in these fusion experiments were analysed by the fluorescence activated cell sorter and karyotyping. An anti-idiotype ELISA assay detected the presence of the patient's characteristic idiotype bearing immunoglobulin in the supernatant of a number of the hybridoma cell lines generated in both fusions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00365082
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  • 9
    Electronic Resource
    Electronic Resource
    The role of smart medical systems in the Space Station (1989)
    Springer
    Journal of clinical monitoring and computing 6 (1989), S. 91-98 
    Details
    ISSN: 1573-2614
    Keywords: expert systems ; computers in medicine ; decision making ; space medicine
    Source: Springer Online Journal Archives 1860-2000
    Topics: Computer Science , Medicine
    Notes: Abstract NASA is developing a Health Maintenance Facility to provide medical equipment and supplies requisite for the Space Station to be launched in the late 1990s. An essential component of this medical facility is a computerized Medical Decision Support System which will expedite medical officers' efforts to maintain the crew's health. The computerized system includes four major functions: 1 A data collection and storage system with a self-contained medical expert scheme for performing treatment protocols. The expert system has ‘data driven’ and ‘time driven’ capabilities to facilitate automatic decision-making functions. 2 An integrated medical record and medical ‘reference’ information management component. 3 An inventory management system for medical supplies and pharmaceuticals. 4 Video, audio, and data communications between the medical officer in the Space Station and ground-based medical personnel. This paper discusses the design of such computerized data collection, communications and expert medical systems as will be developed for use in a Space Station Health Maintenance Facility.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01720419
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  • 10
    Electronic Resource
    Electronic Resource
    Mollicute identified in novel association with aquatic invertebrate (1987)
    Springer
    Current microbiology 15 (1987), S. 85-89 
    Details
    ISSN: 1432-0991
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Prokaryotes lacking a cell wall, formerly known as mycoplasmas were recently designated as the new class, Mollicutes. The association of unidentified “mycoplasma-like organisms” with aquatic animals has been suggested previously on the basis of visual observations alone. We report the pure-culture identification of a true mollicute, anAcholeplasma, that consistently associates with the larvae and adults of the marine bryozoanWatersipora arcuata. This represents the first confirmed nondisease association between a mollicute and an aquatic invertebrate.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01589367
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