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  • Immunohistochemistry  (2)
  • Cytoskeleton  (1)
  • Springer  (3)
  • American Association for the Advancement of Science (AAAS)
  • American Society of Hematology
  • Oxford University Press
  • Springer Nature
  • 2015-2019
  • 1985-1989  (3)
Collection
Publisher
  • Springer  (3)
  • American Association for the Advancement of Science (AAAS)
  • American Society of Hematology
  • Oxford University Press
  • Springer Nature
Years
  • 2015-2019
  • 1985-1989  (3)
Year
  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 258 (1989), S. 373-380 
    ISSN: 1432-0878
    Keywords: Stress fibers ; Mesothelium ; Fluorescent cytochemistry ; Actin filaments ; Cytoskeleton ; Rana catesbeiana (Anura)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Actin-containing cytoplasmic fibers in mesothelial cells of the abdominal wall of the bullfrog, Rana catesbeiana, were visualized by rhodamine-phalloidin staining of en face preparations of mesothelial cells. These fibers ran straight and were aligned parallel with each other. They also showed immunofluorescence staining with antibody against myosin or α-actinin. Electron microscopy revealed the presence of microfilament bundles in the basal cytoplasm of the cells. These cytoplasmic fibers may be comparable to the stress fibers observed in cultured cells. The mesothelial cells of tadpoles initially developed stress fibers when they underwent transformation from a polygonal to a spindle-like shape. Such fibers were also present in the polygonal cells of frogs. Expression of stress fibers in these cells seems to correspond to the expansion of the abdominal wall caused by marked growth of some intraperitoneal organs. The stress fibers in the mesothelial cells may serve to regulate cellular transformation and, further, may play a role in maintaining cellular or epithelial integrity by strengthening the cellular attachment to subepithelial tissue against probable tension load on the abdominal wall.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-0878
    Keywords: Caudal neurosecretory system ; Urotensin ; Neuropeptides ; Teleosts ; Elasmobranchs ; Immunohistochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary In three species of teleosts — carp Cyprinus carpio; grass carp Ctenopharyngodon idella; and crucian carp Carassius auratus — the caudal neurosecretory system displays small, medium-sized and large neurons. Urotensin I (UI)-immunoreactive and UI-nonreactive neurons were found in all three groups; in general, the number of the latter neurons exceeded that of the former. Noteworthy are: (i) UI-immunoreactive fibers in the caudal spinal cord and (ii) dense accumulations of UI-immunoreactive product around the capillaries of the urophysis. In two species of elasmobranchs — cat shark Heterodontus japonicas and swell shark Cephaloscyllium umbratile — neurosecretory neurons decreased in size in rostro-caudal direction. Most of the neurosecretory perikarya, their axons and the corresponding neurohemal areas were UI-immunoreactive, but a small number of secretory neurons was devoid of immunoreaction. Oxytocin, arginine vasopressin, substance P, somatostatin, neurotensin, vasoactive intestinal polypeptide and gastrin-releasing peptide were not detected in the caudal neurosecretory system of the carp.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1432-0878
    Keywords: Neurokinin A ; Substance P ; Dental pulp ; Immunohistochemistry ; Cat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The distribution and origin of neurokinin A (NKA)-like immunoreactivity were investigated in feline dental pulp by an indirect immunofluorescence method. NKA-containing nerve fibres with varicosities, which entered the dental pulp via apical foramen, were distributed throughout this tissue. Many NKA-containing nerve fibres were localized around blood vessels, but some were observed apart therefrom. At the odontoblastic layer, thin NKA-containing nerve fibres were observed running straight toward the pulp-predentinal border between odontoblasts. After inferior alveolar nerve section, all NKA-containing nerve fibres disappeared in the dental pulp, while the removal of the superior cervial ganglion resulted in no change in the distribution of these fibres. The correlation of NKA-like immunoreactivity and substance P (SP)-like immunoreactivity was also investigated by double-immunofluorescence technique. The distribution of NKA-containing nerve fibres was very similar to that of SP-containing nerve fibres; it appeared that all NKA-containing nerve fibres contained SP.
    Type of Medium: Electronic Resource
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