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  • Earth Resources and Remote Sensing  (10)
  • Cell & Developmental Biology  (4)
  • GENERAL
  • 2015-2019  (3)
  • 1990-1994  (12)
  • 1
    Electronic Resource
    Electronic Resource
    Spatial distribution of posttranslationally modified tubulins in polarized cells of developing Artemia (1991)
    New York, NY : Wiley-Blackwell
    Cell Motility and the Cytoskeleton 18 (1991), S. 189-203 
    Details
    ISSN: 0886-1544
    Keywords: microtubules ; isotubulins ; actin ; brine shrimp ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: In many differentiated cells, posttranslationally modified tubulins exhibit restricted subcellular distribution, leading to the proposal that they are required for the production and maintenance of polarity. To study this possibility, we used immunological approaches to examine tubulin isoforms in developing Artemia larvae and to determine their location in several types of cells within the organism. The amount of tubulin in relation to total protein remained relatively constant during early larval development while detyrosinated tubulin increased, an event correlated with the differentiation of larval gut muscle cells. Except for epidermal cells of the developing thorax, each type of cell within the Artemia larvae exhibited characteristic staining patterns which were very similar for each antitubulin antibody. Within epidermal cells, microtubules containing acetylated tubulin appeared patchy or punctate in their distribution, an image not seen with the other antibodies. In most polarized cells, staining for tubulin and actin colocalized in discrete areas, demonstrating enrichment of both proteins within the same cellular compartment and suggesting functional interactions. Mitotic figures were stained with qualitatively equal intensity by all of the antitubulin antibodies, but asters were not observed. Midbodies were intensely stained with phalloidin as well as the antibodies to tubulin. It was clear that microtubules exhibited a preferential localization in cells of Artemia but in no case was a tubulin isoform found exclusively in one area of a cell. The results support the contention that microtubules influence the organization of polarized cell structure and function but they do not permit the conclusion that this capability is dependent on the localization of posttranslationally modified tubulins to restricted subcellular positions.
    Additional Material: 8 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/cm.970180305
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Variations in mitochondrial ultrastructure and dynamics observed by high resolution scanning electron microscopy (HRSEM) (1994)
    New York, NY [u.a.] : Wiley-Blackwell
    Microscopy Research and Technique 27 (1994), S. 269-277 
    Details
    ISSN: 1059-910X
    Keywords: Cristae ; 3D structure ; Hepatocytes ; Fibroblasts ; Adrenal cortex ; Brown fat ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Natural Sciences in General
    Notes: Rat adrenal cortex was processed for high resolution scanning electron microscopy (HRSEM) to confirm tubular cristae, reported by transmission electron microscopy to be present in cortex mitochondria. Mitochondria in several other tissue and cell types were also observed and their ultrastructure confirmed by using three-dimensional, stereo, high resolution scanning electron microscopy. The mitochondria in rat and human hepatocytes as well as human skin fibroblasts mitochondria proved to be long, up to 46 micrometers and branching, as compared to those in liver which were spherical in shape. Cold adapted brown fat cells were packed with mitochondria, these containing plate or shelf-like cristae. Branched, rat striated muscle mitochondria were observed to curve around contractile protein filament bundles. The muscle mitochondrial cristae were found to be both tubular and plate-like, within the same mitochondrion. The ratio of tubular cristae to plate-like cristae varied considerably between muscle mitochondria. In order to use ultrastructural changes in mitochondria for differential diagnosis, and because 3D reconstruction of mitochondria based on transmission electron microscopy serial sections is severely limited in resolution, it is imperative to first develop a correct understanding of tissue specific, normal mitochondrial ultrastructure based on three-dimensional, HRSEM methods. © 1994 Wiley-Liss, Inc.
    Additional Material: 10 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jemt.1070270402
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Culture of a prostatic cell line in basement membrane gels results in an enhancement of malignant properties and constitutive alterations in gene expression (1994)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 158 (1994), S. 325-336 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Interaction of a transformed rat prostate epithelial cell (NbMC-2) with basement membrane gels (Matrigel) has been evaluated using a long-term matrix culture system. NbMC-2 cells, and single-cell clonal derivatives, formed spheroidal multicellular structures (aggregates) on Matrigel surfaces and were weakly invasive or noninvasive during a 1 week period. During subsequent 2-4 week periods, invasive cells originating from the aggregates and exhibiting a fusiform morphology became evident and increased in number in the matrix cultures. This biphasic pattern of behavior did not occur on laminin, type I or type IV collagen, or fibronectin substrates, but it did occur on Matrigel in serum-free medium. Characterization of sublines enriched in fusiform cells indicated that they maintained their distinct morphology with continuous culture. Further, they exhibited significantly greater invasive potential, saturation density, and random motility (chemokinesis) than the parent cell line. Steady-state levels of fibronectin mRNA were highly elevated in the tusiform variants, demonstrating a constitutive alteration in patterns of gene expression coinciding with the altered morphology. These results indicate that clonal NbMC-2 cells differentiate at a reproducible frequency into a more aggressive cell type in response to culture in the basement membrane--like matrix. The altered phenotypic properties appear to be stable since they can be inherited by daughter cells and because they are evident in the absence or matrix. These observations suggest a cell-specific mechanism for promotion of malignant growth by matrix-mediated induction of novel cell properties. © 1994 Wiley-Liss, Inc.
    Additional Material: 9 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1041580215
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  • 4
    Electronic Resource
    Electronic Resource
    Cell cycle regulated expression of nucleolar antigen P120 in normal and transformed human fibroblasts (1993)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 154 (1993), S. 16-27 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Normal and SV40 virus-transformed WI-38 human lung fibroblasts were serum starved and refed, or synchronized by double thymidine block and released from the block. At different time points in the cell cycle, steady state levels of P120 mRNA and P120 protein content of the cells were determined by densitometric scans of Northern and Western blots. At the same time points, [3H]thymidine uptake was measured and flow cytometric analysis performed for DNA content and P120 antigen staining. Levels of P120 protein and P120 mRNA were approximately 4 times greater in non-synchronous, exponentially growing transformed cells than in similarly growing normal cells. Early G1-cells, synchronized either with serum deprivation or with metabolic block, contained only a trace amount of P120 protein and mRNA. The P120 gene was transcribed early in G1 and P120 protein synthesis initiated in middle G1. A dramatic increase of P120 protein level occurred in S-phase with a corresponding mRNA peak preceding the P120 protein peak. These results indicate that P120 is overexpressed in transformed WI-38 cells and that P120 is temporally regulated during the cell cycle of both transformed and normal fibroblasts. The dramatic increase in P120 protein expression at the G1 to S boundary suggests that P120 may play a role in the regulation of cell cycle and increased nucleolar activity that is associated with cell proliferation. © 1993 Wiley-Liss, Inc.
    Additional Material: 9 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1041540104
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  • 5
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    Development of SIR-C Ground Calibration Equipment (1993)
    In:  Other Sources
    Details
    Publication Date: 2018-06-08
    Description: SIR-C/X-SAR is currently scheduled for launch in April 1994. SIR-C is an L-Band and C-Band, multi-polarization spaceborne SAR system developed by NASA/JPL. X- SAR is an X-Band SAR system developed by DARA/ASI. One of the problems involved in calibrating the SIR-C instrument is to make sure that the horizontal (H) and vertical (V) polarized beams are aligned in the azimuth direction, i.e.. that they are pointing in the same direction. This is important if the polarimetric performance specifications for the system are to be met. To solve this problem, we have designed and built a prototype of a low-cost ground receiver capable of recording received power from two antennas, one H-polarized, the other V-polarized. The two signals are mixed to audio then recorded on the left and right stereo channels of a standard audio cassette player. The audio cassette recording can then be played back directly into a Macintosh computer, where it is digitized. Analysis of.
    Keywords: Earth Resources and Remote Sensing
    Type: IEEE, Geoscience and Remote Sensing; Tokyo; Japan
    Format: text
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  • 6
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    The Effects of Noise on Polarimetric SAR Data (1993)
    In:  Other Sources
    Details
    Publication Date: 2018-06-08
    Description: Polarimetric SAR data can provide a great deal of information about the scattering behavior of the surface under observation. Polarimetric SAR systems often measure the scattering matrices of the areas under observation in linear polarizations (H and V). From the scattering matrix commonly used forms such as the covariance matrix and the Stokes matrix can be easily derived. Other measures derived from polarimetric SAR data include the standard deviation of texture, correlation coefficients between scattering matrix terms, and the mode and variance of phase differences between scattering matrix terms. The effects of additive system noise on these measurements is not often considered in the literature on this subject.
    Keywords: Earth Resources and Remote Sensing
    Type: IEEE, Geoscience & Remote Sensing; Tokyo; Japan
    Format: text
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  • 7
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    Calibration Results for J-ERS-1 SAR Data Produced by the Alaska SAR Facility (1993)
    In:  Other Sources
    Details
    Publication Date: 2018-06-08
    Description: The Alaska SAR Facility has been receiving and processing SAR data from the J-ERS-1 satellite since Spring 1992. Corner reflectors have been set up for J-ERS-1 SAR calibration at a site near Delta Junction, in central Alaska. Image quality and calibration analysis results from the Delta Junction site and others will be presented in this paper. The impact of the 3-bit Analog-to-Digital Converter and the automatic stepping of the gain as a function of range in the J-ERS-1 radar receiver on calibration performance has been assessed. Preliminary observations on J-ERS-1 SAR data are that the average Signal-to-Noise ratio is generally fairly low, in the range 5-6dB. Azimuth ambiguity levels are higher than preflight analysis indicated. Over land, the dynamic range in the backscatter at L-band for approximately 36 degree incidence angle is often fairly high.
    Keywords: Earth Resources and Remote Sensing
    Type: IEEE, Geoscience and Remote Sensing; Tokyo; Japan
    Format: text
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  • 8
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    Calibration Results for J-ERS-1 SAR Data Produced by the Alaska SAR Facility (1993)
    In:  Other Sources
    Details
    Publication Date: 2018-06-08
    Description: The Alaska SAR Facility has been receiving and processing SAR data from the J-ERS-1 satellite since Spring 1992. Corner reflectors have been set up for J-ERS-1 SAR calibration at a site near Delta Junction, in central Alaska. Image quality and calibration analysis results from the Delta Junction site and others will be presented in this paper. The impact of the 3-bit Analog-to-Digital Converter and the automatic stepping of the gain as a function of range in the J-ERS-1 radar receiver on calibration performance has been assessed. Preliminary observations on J-ERS-1 SAR data are that the average Signal-to-Noise ratio is generally fairly low, in the range 5-6 dB. Azimuth ambiguity levels are higher than preflight analysis indicated. Over land, the dynamic range in the backscatter at L-band for approximately 36 degree incidence angle is often fairly high...
    Keywords: Earth Resources and Remote Sensing
    Type: IEEE, Geoscience & Remote Sensing; Tokyo; Japan
    Format: text
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  • 9
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    The Effects of Noise on Polarimetric SAR Data (1993)
    In:  Other Sources
    Details
    Publication Date: 2018-06-08
    Description: Polarimetric SAR data can provide a great deal of information about the scattering behavior of the surface under observation. Polarimetric SAR systems often measure the scattering matrices of the areas under observation in linear polarizations (H and V). From the scattering matrix commonly used forms such as the covariance matrix and the Stokes matrix can be easily derived. Other measures derived from polarimetric SAR data include correlation coefficients between scattering matrix terms and the mode and variance of phase differences between scattering matrix terms. The effects of additive system noise on these measurements is not often considered in the literature on this subject. In this paper, the effects of additive system noise on measurements derived from polarimetric SAR data will be examined. It will be shown how first-order noise effects can be removed and how second-order noise effects can be reduced for some measurements...
    Keywords: Earth Resources and Remote Sensing
    Type: IEEE, Geoscience & Remote Sensing; Tokyo; Japan
    Format: text
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  • 10
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    Remote Sensing of Tropical Forests with Multi-Frequency Polarimetric Radar (AIRSAR) as an Aid in Vegetation Classification (1994)
    In:  Other Sources
    Details
    Publication Date: 2018-06-08
    Description: Vegetation maps of inaccessible areas in the tropics tend to divide vegetation into broad types.
    Keywords: Earth Resources and Remote Sensing
    Type: German Botanical Congress; Bayreuth; Germany
    Format: text
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