ISSN:
1573-6849
Keywords:
azacytidine
;
condensation
;
human cytogenetics
;
FISH
;
satellite DNA
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Fluorescencein situ hybridization employing human alphoid, beta and classical satellite DNA probes was performed on 5-azacytidine treated and untreated chromosomes obtained from human lymphocytes. The individual used in this study presented a polymorphism of constitutive heterochromatin of chromosomes 1 and 9 as revealed byin situ digestion with the restriction endonucleaseAlul. Neither the alphoid nor the beta satellite DNA domains were susceptible to condensation-inhibition by 5-azacytidine. Only the classical satellite localized on chromosome 9 was affected. The constitutive heterochromatin size polymorphism was shown to depend mainly on variations of the classical satellite DNA domain. Therefore, condensation-inhibition, as a phenomenon which may modify the natural folding of the chromatin fibre, regionally affects human constitutive heterochromatin and seems to be dependent on the heterochromatic family.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01539451
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