ISSN:
1600-5775
Source:
Crystallography Journals Online : IUCR Backfile Archive 1948-2001
Topics:
Geosciences
,
Physics
Notes:
The determination of the structure of the transcriptionally active core particle of bluetongue virus is discussed. This particle is approximately 700 Å in diameter and reasonably well ordered, but fragile, crystals have been obtained from two different serotypes of the virus. Cryocrystallography proved difficult and a large number of crystals were analysed at room temperature to accumulate a reasonably complete data set. The effects of synchrotron optics, station design and detector on the signal-to-noise for these weak data are discussed, with particular reference to station ID2 at the European Synchrotron Radiation Facility. Once the data had been gathered, structure determination was straightforward, using a model derived from a combination of electron microscopy and protein crystallography to obtain initial phases. Despite apparent isomorphism, it is suspected that the crystal lattice `ages', perhaps reflecting both the inevitable weakness of the forces holding crystals of such a large macromolecular complex together and flexibility in the particle.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1107/S090904959900669X
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