ISSN:
0021-9541
Keywords:
Life and Medical Sciences
;
Cell & Developmental Biology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Biology
,
Medicine
Notes:
The growth characteristics of a newly established cell line, Hep40, derived from a human hepatoma are described. An absolute requirement was found for serum to mediate cell growth. Neither EGF, TGF-α, nor HGF altered cell growth in the presence or absence of serum. A partial suppression of cell growth was achieved by several TGF-β family proteins. Affinity crosslinking gels using 125I-labeled TGF-β showed a significant decrease in the TGF-β cell-surface type II receptor in Hep40 cells, compared to the TGF-β-sensitive Hep3B cell line. However, growth could be completely suppressed by addition of vitamins K to the culture medium in both Hep40 and several other hepatoma cell lines. Growth suppression by vitamins K was accompanied by an increased level of transcripts for c-myc, c-jun, and prothrombin genes, in contrast to the actions of TGF-β1 protein, which caused a decrease in the level of c-myc transcripts. These data show that this new human hepatoma cell line has partial resistance to growth inhibition by TGF-β with a unique TGF-β receptor defect. However, growth was completely suppressed by vitamins K. The differing gene expression patterns in response to TGF-β as compared to vitamin K suggest that these two growth inhibitors act through differing pathways. © 1995 Wiley-Liss Inc.
Additional Material:
11 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/jcp.1041650303
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