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  • Phaffia rhodozyma  (3)
  • Springer  (3)
  • Blackwell Publishing Ltd
  • 2015-2019
  • 1995-1999  (3)
Collection
Keywords
Publisher
  • Springer  (3)
  • Blackwell Publishing Ltd
Years
  • 2015-2019
  • 1995-1999  (3)
Year
  • 1
    Electronic Resource
    Electronic Resource
    Genetics and electrophoretic karyotyping of wild-type and astaxanthin mutant strains of Phaffia rhodozyma (1997)
    Springer
    Antonie van Leeuwenhoek 72 (1997), S. 111-117 
    Details
    ISSN: 1572-9699
    Keywords: astaxanthin ; carotene ; karyotype ; Phaffia rhodozyma
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In this work we establish the chromosomal composition of a wild-type, one astaxanthin and two β-carotene overproducer strains of the red yeast Phaffia rhodozyma. The method used has been pulsed field gel electrophoresis, which has determined 9 DNA chromosomal bands in the yeast genome. The two largest bands are triplets and two other bands, VI and VIII, seem to be doublets. The size of the chromosomal bands varies between 0.35 and 2.5 Mb, suggesting a genome size of 25 Mb. The technique used, complemented with hybridization assays using specific DNA probes, provides direct information about the genomic organization of P. rhodozyma. We have also cloned and located in chromosomal bands different DNA sequences that code for the translation elongation factor 1 alpha (ef-1α), a 7.6 kb BamHI fragment of repetitive DNA (possibly rDNA) and a randomly chosen fragment (named locus R2). Additionally, we have detected a chromosomal length polymorphism between wild-type strains and mutant strains affecting carotenogenesis obtained in our laboratory.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1000200119447
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  • 2
    Electronic Resource
    Electronic Resource
    Genetic transformation of astaxanthin mutants of Phaffia rhodozyma (1998)
    Springer
    Antonie van Leeuwenhoek 73 (1998), S. 147-153 
    Details
    ISSN: 1572-9699
    Keywords: transformation ; plasmid integration ; Phaffia rhodozyma
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Stable red astaxanthin-producing transformants were obtained after genetic transformation of two Phaffia rhodozyma mutants. A yellow mutant, accumulating β-carotene, and an albino mutant, accumulating phytoene, from P. rhodozyma were transformed using a genomic library of wild-type strain UCD 67-385 in the pBluescript vector. Hybridization assays, using the pBluescript DNA as a radioactive probe, indicate integration of vector sequences into the genome of the transformants. Transformants DNA was digested with restriction endonucleases, ligated with T4 DNA ligase and then used to transform E. coli. Ampicillin resistant plasmids, containing 0.1, 0.2, and 2.5 kb DNA inserts of P. rhodozyma, were rescued from the yeast red transformants. The molecular analysis indicate that transformation has occurred by an integration event of donor DNA into the genome of the host strains.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1000602110765
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  • 3
    Electronic Resource
    Electronic Resource
    Complementation analysis with new genetic markers in Phaffia rhodozyma (1998)
    Springer
    Antonie van Leeuwenhoek 73 (1998), S. 229-236 
    Details
    ISSN: 1572-9699
    Keywords: auxotrophs ; mutagenesis ; nystatin enrichment ; rotoplasts fusion ; Phaffia rhodozyma
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Isolation and characterization of auxotrophic mutants from wild-type and astaxanthin mutant strains of Phaffia rhodozyma is described. Differences in survival were observed when u.v. irradiation of P. rhodozyma wild-type and astaxanthin mutant strains were incubated in the dark or exposed to photoreactivating light. Ultra-violet mutagenesis was not effective to produce auxotrophic mutants in this yeast. Auxotrophic mutants were obtained with high efficiency through a nystatin enrichment procedure after a N-methyl-N′-nitro-N-nitrosoguanidine (NTG) mutagenic treatment with a 0.12% survivor level. Stringent mutagenetic conditions were needed to obtain P. rhodozyma auxotrophs. The most frequent mutants were ade- and met- in a rather narrow auxotroph spectrum. These results may be associated with a possible diploid condition of this yeast. The high number of adenine auxotrophs obtained in relation to other auxotrophic mutants suggests the possibility of some degree of heterozygosity in the wild-type strain UCD 67-385.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1000828220033
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