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  • Articles  (17)
  • Springer  (12)
  • MDPI  (4)
  • Cambridge University Press  (1)
  • ZBW - Deutsche Zentralbibliothek für Wirtschaftswissenschaften, Leibniz-Informationszentrum Wirtschaft Kiel, Hamburg
  • 2015-2019  (7)
  • 1995-1999  (4)
  • 1985-1989  (4)
  • 1975-1979  (2)
Collection
  • Articles  (17)
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Year
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  • 1
    Electronic Resource
    Electronic Resource
    Fine structure of OXI1, the mitochondrial gene coding for subunit II of yeast cytochrome c oxidase (1979)
    Springer
    Current genetics 1 (1979), S. 75-83 
    Details
    ISSN: 1432-0983
    Keywords: Genetic mapping ; Mutant polypeptides ; Direction of translation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Genetic and biochemical studies have been performed with 110 mutants which are defective in cytochrome a·a3 and map in the regions on mit DNA previously designated OXI1 and OXI2. With 88 mutations allocated to OXI1 fine structure mapping was achieved by the analysis of rho − deletions. The order of six groups of mutational sites (A 1, A2, B 1, B2, C 1, C2) thus determined was confirmed by oxi i x oxi j recombination analysis. Analysis of mitochondrially translated polypeptides of oxil mutants by SDS-polyacrylamide electrophoresis reveals three classes of mutant patterns: i) similar to wild-tpye (19 mutants); ii) lacking SU II of cytochrome c oxidase (53 mutants); iii) lacking this subunit and exhibiting a single new polypeptide of lower Mr (16 mutants). Mutations of each of these classes are scattered over the OXI1 region without any detectable clustering; this is consistent with the assumption that all oxil mutations studied are within the same gene. New polypeptides observed in oxil mutants of class iii) vary in Mr in the range from 10,500 to 33,000. Those of Mr 17,000 to 33,000 are shown to be antigenically related to subunit II of cytochrome c oxidase. Colinearity is established between the series of new polypeptides of Mr values increasing from 10,500 to 31,500 and the order of the respective mutational sites on the map, e.g. mutations mapping in A 1 generate the smallest and mutations mapping in C2 the largest mutant fragments. From these data we conclude that i) all mutations allocated to the OXI1 region are in the same gene; ii) this gene codes for subunit II of cytochrome c oxidase; iii) the direction of translation is from CAP to 0X12. Out of 19 mutants allocated to OXI2 three exhibit a new polypeptide; these and all the other oxi2 mutants lack subunit III of cytochrome oxidase. This result provides preliminary evidence that the OXI2 region harbours the structural gene for this subunit III.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00413308
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  • 2
    Electronic Resource
    Electronic Resource
    Rapid reactions of spruce cells to elicitors released from the ectomycorrhizal fungus Hebeloma crustuliniforme, and inactivation of these elicitors by extracellular spruce cell enzymes (1996)
    Springer
    Planta 198 (1996), S. 118-126 
    Details
    ISSN: 1432-2048
    Keywords: Ectomycorrhiza ; Elicitor inactivation ; Elicitor-induced reaction ; Hebeloma — Picea cells ; Signal transduction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Elicitors released from hyphae or cell walls of the ectomycorrhizal fungus Hebeloma crustuliniforme (Bull. ex Fries.) Quél. induced in suspension-cultured cells of Picea abies (L.) Karst. a set of fast reactions: (i) an immediate efflux of Cl− into the medium, followed by a K+ efflux; (ii) an influx of Ca2+ (measured as accumulation of 45Ca2+ in the cells); (iii) a phosphorylation of a 63-kDa protein and dephosphorylation of a 65-kDa protein (detectable by 4 min after elicitor application); (iv) an alkalinization of the medium, and (v) a transient synthesis of H2O2. The removal of extracellular Ca2+ by EGTA delayed the elicitor-induced alkalinization. A further reduction of this response could be achieved by TMB-8 an inhibitor of Ca2+ release from intracellular stores. Moreover, the inhibition of protein kinase activity by staurosporine prevented the extracellular alkalinization completely. However, the effectiveness of the elicitors in inducing the extracellular alkalinization was strongly impaired by constitutively secreted enzymes of spruce cells which cleaved the elicitors to inactive fragments. It is suggested that in ectomycorrhizae the efficacy of elicitors released from fungal cell walls is controlled by apoplastic enzymes of the host; the plant itself is able to reduce the activity of fungal elicitors on their way through the plant cell wall. But those elicitors which finally reach the plasma membrane of host cells induce reactions that are similar to the early defense reactions in plant-pathogen interactions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00197594
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  • 3
    Electronic Resource
    Electronic Resource
    Druckaufschluß im Mikrowellenofen (1987)
    Springer
    Fresenius' Zeitschrift für analytische Chemie 328 (1987), S. 82-84 
    Details
    ISSN: 1618-2650
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary Decompositions of inorganic and organic materials can be carried out within a few minutes by means of the microwave technique. For dissolving substances with volatile components in the microwave oven, low pressure containers were developed from microwave transparent materials and an excess pressure security system. Results and limitations of the method are explained by examples.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00560955
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  • 4
    Electronic Resource
    Electronic Resource
    Mutation of a highly conserved base in the yeast mitochondrial 21S rRNA restricts ribosomal frameshifting (1995)
    Springer
    Molecular genetics and genomics 248 (1995), S. 207-216 
    Details
    ISSN: 1617-4623
    Keywords: Chloramphenicol resistance ; frameshifting Mitochondria ; 21S rRNA ; Yeast
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A mutation shown to cause resistance to chloramphenicol inSaccharomyces cerevisiae was mapped to the central loop in domain V of the yeast mitochondrial 21S rRNA. The mutant 21S rRNA has a base pair exchange from U2677 (corresponding to U2504 inEscherichia coli) to C2677, which significantly reduces rightward frameshifting at a UU UUU UCC A site in a + 1 U mutant. There is evidence to suggest that this reduction also applies to leftward frameshifting at the same site in a − 1 U mutant. The mutation did not increase the rate of misreading of a number of mitochondrial missense, nonsense or frameshift (of both signs) mutations, and did not adversely affect the synthesis of wild-type mitochondrial gene products. It is suggested here that ribosomes bearing either the C2677 mutation or its wild-type allele may behave identically during normal decoding and only differ at sites where a ribosomal stall, by permitting non-standard decoding, differentially affects the normal interaction of tRNAs with the chloramphenicol resistant domain V. Chloramphenicol-resistant mutations mapping at two other sites in domain V are described. These mutations had no effect on frameshifting.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02190802
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  • 5
    Electronic Resource
    Electronic Resource
    Additive cytotoxicity of adriamycin and a naturally occurring growth inhibitor extracted from bovine aorta (1985)
    Springer
    Investigational new drugs 3 (1985), S. 23-29 
    Details
    ISSN: 1573-0646
    Keywords: growth inhibitor ; A-10 ; carcinoma ; mammary ; antineoplastic chemotherapy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Medicine
    Notes: Abstract A factor of nominal molecular weight 6K–10K Daltons, isolated from bovine aorta, has previously been shown to inhibit neovascularization and tumor growth in vivo and the growth of some tumor cells as well as endothelial cells in culture. This factor, termed A-10, was tested alone and in combination with Adriamycin against TA3Ha mammary adenocarcinoma cells in tissue culture. It was found to have cytotoxicity additive to that of Adriamycin in inhibiting the growth of these cells. In vitro and animal studies show that the sequence of Adriamycin → A-10 is superior to either agent alone in delaying the appearance of palpable tumors after subcutaneous injection of 105 pre-treated tumor cells in the tail of strain A mice. While the growth rate of the primary tumor was not affected by such treatment, survival was prolonged to a greater degree by the this sequence than by either of these agents used alone. A-10 treatment reduced the number of metastases to the adrenal gland but not to lung, liver, or lymph nodes. It did, however, reduce the size of metastases to para-aortic lymph nodes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00176820
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  • 6
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    IJERPH, Vol. 16, Pages 3399: Leg Dominance as a Risk Factor for Lower-Limb Injuries in Downhill Skiers—A Pilot Study into Possible Mechanisms (2019)
    MDPI
    Details
    Publication Date: 2019
    Description: Leg dominance has been reported as one potential risk factor for lower-limb injuries in recreational downhill skiers. The current study proposed and tested two possible mechanisms for a leg dominance effect on skiing injuries—imbalance of the knee muscle strength and bilateral asymmetry in sensorimotor control. We hypothesized that the knee muscle strength (Hypothesis 1; H1) or postural control (Hypothesis 2; H2) would be affected by leg dominance. Fifteen well-experienced recreational downhill skiers (aged 24.3 ± 3.2 years) participated in this study. Isometric knee flexor/extensor muscle strength was tested using a dynamometer. Postural control was explored by using a kinematic principal component analysis (PCA) to determine the coordination structure and control of three-dimensional unipedal balancing movements while wearing ski equipment on firm and soft standing surfaces. Only H2 was supported when balancing on the firm surface, revealing that when shifting body weight over the nondominant leg, skiers significantly changed the coordination structure (p 〈 0.006) and the control (p 〈 0.004) of the lifted-leg movements. Based on the current findings, bilateral asymmetry in sensorimotor control rather than asymmetry in strength seems a more likely mechanism for the previously reported effect of leg dominance on lower-limb injury risk in recreational downhill skiers.
    Print ISSN: 1661-7827
    Electronic ISSN: 1660-4601
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Medicine
    Published by MDPI
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  • 7
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    Entropy, Vol. 21, Pages 614: Changed Temporal Structure of Neuromuscular Control, Rather Than Changed Intersegment Coordination, Explains Altered Stabilographic Regularity after a Moderate Perturbation of the Postural Control System (2019)
    MDPI
    In: Entropy
    Details
    Publication Date: 2019
    Description: Sample entropy (SaEn) applied on center-of-pressure (COP) data provides a measure for the regularity of human postural control. Two mechanisms could contribute to altered COP regularity: first, an altered temporal structure (temporal regularity) of postural movements (H1); or second, altered coordination between segment movements (coordinative complexity; H2). The current study used rapid, voluntary head-shaking to perturb the postural control system, thus producing changes in COP regularity, to then assess the two hypotheses. Sixteen healthy participants (age 26.5 ± 3.5; seven females), whose postural movements were tracked via 39 reflective markers, performed trials in which they first stood quietly on a force plate for 30 s, then shook their head for 10 s, finally stood quietly for another 90 s. A principal component analysis (PCA) performed on the kinematic data extracted the main postural movement components. Temporal regularity was determined by calculating SaEn on the time series of these movement components. Coordinative complexity was determined by assessing the relative explained variance of the first five components. H1 was supported, but H2 was not. These results suggest that moderate perturbations of the postural control system produce altered temporal structures of the main postural movement components, but do not necessarily change the coordinative structure of intersegment movements.
    Electronic ISSN: 1099-4300
    Topics: Chemistry and Pharmacology , Physics
    Published by MDPI
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  • 8
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    Agronomy, Vol. 9, Pages 214: Effect of Salinity and Water Stress on the Essential Oil Components of Rosemary (Rosmarinus officinalis L.) (2019)
    MDPI
    In: Agronomy
    Details
    Publication Date: 2019
    Description: The effect of salinity and water stresses on the essential oil components of Rosmarinus officinalis essential oil was investigated. Rosemary plants were submitted to different water treatments: tap water (TW), salt water (SW) and without irrigation (NIR). GC/MS analysis showed that ten and eleven volatile compounds were identified in essential oil of rosemary plants irrigated with tap water (TW) and salt water (SW), respectively. However, thirteen volatile compounds were identified in essential oil of non-irrigated plants (NIR). Moreover, among these compounds, α-Pinene, Eucalyptol (1,8 Cineol), Camphene, Borneol, D-verbenone, Bornyl acetate were the major components of oil. Also, GC/MS results highlighted that non-irrigated rosemary plants showed the highest essential oil yield (Y). Obtained oil yields followed the order YNIR 〉 YTW 〉 YSW. In conclusion, qualitative and quantitative differences in rosemary essential oil components were highlighted in relation to water stress.
    Electronic ISSN: 2073-4395
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Economics
    Published by MDPI
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  • 9
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    Entropy, Vol. 21, Pages 70: The Effect of Cognitive Resource Competition Due to Dual-Tasking on the Irregularity and Control of Postural Movement Components (2019)
    MDPI
    In: Entropy
    Details
    Publication Date: 2019
    Description: Postural control research suggests a non-linear, n-shaped relationship between dual-tasking and postural stability. Nevertheless, the extent of this relationship remains unclear. Since kinematic principal component analysis has offered novel approaches to study the control of movement components (PM) and n-shapes have been found in measures of sway irregularity, we hypothesized (H1) that the irregularity of PMs and their respective control, and the control tightness will display the n-shape. Furthermore, according to the minimal intervention principle (H2) different PMs should be affected differently. Finally, (H3) we expected stronger dual-tasking effects in the older population, due to limited cognitive resources. We measured the kinematics of forty-one healthy volunteers (23 aged 26 ± 3; 18 aged 59 ± 4) performing 80 s tandem stances in five conditions (single-task and auditory n-back task; n = 1–4), and computed sample entropies on PM time-series and two novel measures of control tightness. In the PM most critical for stability, the control tightness decreased steadily, and in contrast to H3, decreased further for the younger group. Nevertheless, we found n-shapes in most variables with differing magnitudes, supporting H1 and H2. These results suggest that the control tightness might deteriorate steadily with increased cognitive load in critical movements despite the otherwise eminent n-shaped relationship.
    Electronic ISSN: 1099-4300
    Topics: Chemistry and Pharmacology , Physics
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  • 10
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    Druckaufschluß im Mikrowellenofen (1987)
    Springer
    Details
    Publication Date: 1987-01-01
    Print ISSN: 0016-1152
    Topics: Chemistry and Pharmacology
    Published by Springer
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