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  • Articles  (4)
  • Bone resorption  (3)
  • 41 A 10  (1)
  • Springer  (4)
  • 2015-2019
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  • 1975-1979  (3)
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  • Articles  (4)
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  • Springer  (4)
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  • 2015-2019
  • 1995-1999  (1)
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  • 1
    ISSN: 1432-0827
    Keywords: Nerve growth factor ; Bone resorption ; Parathyroid hormone ; Insulin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Summary The effects of 7S nerve growth factor (NGF) and its isolated α, β, and γ subunits on bone resorption were assessed in a tissue culture system in which the degree of resorption was determined by measuring the release of45Ca from prelabeled fetal rat radii and ulnae. It was found that 7S-NGF, through the activity of its γ, subunit, inhibits parathyroid hormone (PTH)-stimulated but not-unstimulated bone resorption. The following observations suggest that γ-NGF, a trypsin-like molecule, blocks PTH-induced bone resorption by enzymatic degradation of PTH: (a) γ-NGF does not inhibit bone resorption stimulated by the steroid, 1,25-dihydroxycholecalciferol; (b) trypsin is as effective as γ-NGF in inhibiting PTH-stimulated bone resorption; (c) the PTH-inhibitory action of both γ-NGF and trypsin are eliminated by inactivating these enzymes with diisopropyl fluorophosphate; and (d) addition of γ-NGF to the cultures 2 days after the inclusion of PTH does not result in inhibition of bone resorption. Similarly, when the subunit is added to the culture medium before the hormone, there is no inhibition of resorption. The latter observation suggests that the target of γ-NGF is the PTH molecule rather than its membrane receptors. Crystalline bovine insulin inhibits the γ-NGF suppression of PTH-induced bone resorption. This effect, however, is not mimicked by the addition of zinc, which is present in commerical insulin preparations, to the culture medium. Consequently, insulin must inhibit NGF by some mechanism other than the influence of zinc.
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Calcified tissue international 25 (1978), S. 233-240 
    ISSN: 1432-0827
    Keywords: Bone ; Bone resorption ; Albumin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Summary A fraction (brA), which causes resorption of fetal rat bones in vitro, has been concentrated from bovine serum albumin by anion exchange column chromatography on DEAE Sephadex. This active fraction has also been prepared using DEAE Sephadex A-50 by a batch method with a 0.09M NaCl, 0.1M TRIS buffer, pH 8.35. BrA was 10–30 times more potent than the original albumin. The retained material, which constitutes the bulk of the protein and has less activity than the original albumin, elutes with 0.45M NaCl. Similar treatment of serumα,β or γ globulins does not yield brA. Further enhancement of the bone resorbing activity of brA can be obtained with (NH4)2SO4 fractionation or extraction with CH3OH∶CHCl3. Heating at 55° C for 2 h or at 100° C for 10 min does not affect the activity; overnight incubation with protease destroys the bone resorbing effect. The bone resorbing activity is not removed by dialysis and does not correlate with the protease activity of the fraction. The action of brA is inhibited by 3 mM PO4, 1 μg/ml calcitonin or glucagon, 10−7 M dexamethasone or 0.02 μg/ml actinomycin D. The bone resorbing activity of brA is partially inhibited by 10−7–10−5 M indomethacin. PTH did not elicit bone resorption when added to cultures incubated in chemically defined medium supplemented with 0.1 mg/ml brA. However, brA did not inhibit PTH-induced resorption.
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Calcified tissue international 27 (1979), S. 255-261 
    ISSN: 1432-0827
    Keywords: Macrophage ; Bone resorption ; Tissue culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Summary Because of the difficulty in obtaining large, relatively pure populations of osteoclasts, most studies of bone resorption are performed on intact animals or in cultures of embryonic bone rudiments. These experimental systems, however, do not permit detailed analysis of the cellular mechanisms of matrix degradation or of the means whereby resorbing cells attach to the bone surface. Mononuclear phagocytes, which are probably ontogenetically related to the osteoclast, will resorb bone matrix in tissue culture. Consequently, we have developed an in vitro system whereby the ability of these cells to bind and resorb skeletal matrix can be precisely and individually measured using radioisotopically labeled, devitalized rat bone particles. We have found that when derived from mice, peritoneal macrophages bind approximately 80% of bone particles within the first 40 min of incubation. Significant (P〈0.025) net matrix degradation, as defined by the percentage of isotope released from bone cultured with macrophages as compared to that released in the absence of cells, occurs within the first 3 h of culture and proceeds rapidly for at least the first 2 days of incubation. By this time 40%–50% of isotope usually has been released into the medium. Resident peritoneal macrophages appear to mobilize matrix as actively as those which are thioglycollate induced. By comparison, lymphocytes elicit little isotope mobilization from bone, and rat peritoneal exudate macrophages are markedly less efficient (P〈0.001) at resorbing rat bone than are macrophages obtained from mice. Isotope release by peritoneal macrophages represents true cell-mediated resorption and not merely nonspecific mineral mobilization as evidenced by the facts that: (a) the magnitudes of release of isotopes representing the inorganic (45CaCl) and organic (3H-proline) phases of bone are the same, (b) daily buffering of the cultures to pH 7.4 has little effect on45Ca release, and (c) cell-matrix contact is required for optimal mobilization of45Ca or3H.
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Combinatorica 16 (1996), S. 465-477 
    ISSN: 1439-6912
    Keywords: 05 A 15 ; 05 A 16 ; 05 A 20 ; 60 C 05 ; 41 A 10 ; 68 R
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mathematics
    Notes: Abstract It is often required to find the probability of the union of givenn eventsA 1 ,...,A n . The answer is provided, of course, by the inclusion-exclusion formula: Pr(∪A i )=∑ i −∑ i〈j Pr(A i ∩A j )±.... Unfortunately, this formula has exponentially many terms, and only rarely does one manage to carry out the exact calculation. From a computational point of view, finding the probability of the union is an intractable, #P-hard problem, even in very restricted cases. This state of affairs makes it reasonable to seek approximate solutions that are computationally feasible. Attempts to find such approximate solutions have a long history starting already with Boole [1]. A recent step in this direction was taken by Linial and Nisan [4] who sought approximations to the probability of the union, given the probabilities of allj-wise intersections of the events forj=1,...k. The developed a method to approximate Pr(∪A i ), from the above data with an additive error of exp $$( - O(k/\sqrt n ))$$ . In the present article we develop an expression that can be computed in polynomial time, that, given the sums ∑|S|=j Pr(∩ i∈S A i ) forj=1,...k, approximates Pr(∪A i ) with an additive error of exp $$( - \bar \Omega (k^2 /n))$$ . This error is optimal, up to the logarithmic factor implicit in the $$\bar \Omega$$ notation. The problem of enumerating satisfying assignments of a boolean formula in DNF formF=v l m C i is an instance of the general problem that had been extensively studied [7]. HereA i is the set of assignments that satisfyC i , and Pr(∩ i∈S A i )=a S /2n where ∧ i∈S C i is satisfied bya S assignments. Judging from the general results, it is hard to expect a decent approximation ofF's number of satisfying assignments, without knowledge of the numbersa S for, say, all cardinalities $$1 \leqslant |S| \leqslant \sqrt m$$ . Quite surprisingly, already the numbersa S over |S|≤log(n+1)uniquely determine the number of satisfying assignments for F. We point out a connection between our work and the edge-reconstruction conjecture. Finally we discuss other special instances of the problem, e.g., computing permanents of 0,1 matrices, evaluating chromatic polynomials of graphs and for families of events whose VC dimension is bounded.
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