ISSN:
1617-4623
Keywords:
Key words Basidiomycetes
;
Hygromycin
;
β-Glucuronidase
;
EF-1α promoter
;
Particle bombardment
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract The biotrophic rust fungus Puccinia graminis f. sp. tritici (Pgt) was transformed by particle bombardment. The promoter from the Pgt translation elongation factor 1α (EF-1α) gene was fused to the bacterial marker genes hygromycin B phosphotransferase (hpt) and β-glucuronidase (GUS). Transformation constructs were introduced into uredospores of Pgt, an obligate pathogen of wheat, by biolistic bombardment. Uredospores transformed with the construct containing the hpt gene germinated and initiated branching on selective medium, indicating that they had acquired resistance to hygromycin B. However, transformants stopped growing 5 days after bombardment. GUS activity in uredospores and germlings was histochemically detected 4–16 h after bombardment. GUS expression was also obtained using the INF24 promoter from the bean rust fungus Uromyces appendiculatus, demonstrating that heterologous genes can be expressed in P. graminis under the control of regulatory sequences from closely related organisms.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/PL00008658
Permalink
