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  • 1
    Electronic Resource
    Electronic Resource
    An Attempt to Map Two Genes for Tuberous Sclerosis Using Novel Two-Point Methods (1991)
    Oxford, UK : Blackwell Publishing Ltd
    Annals of the New York Academy of Sciences 615 (1991), S. 0 
    Details
    ISSN: 1749-6632
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Natural Sciences in General
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1749-6632.1991.tb37771.x
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  • 2
    Electronic Resource
    Electronic Resource
    ATP-dependent carbon transport in perfused Chara cells (1993)
    Oxford, UK : Blackwell Publishing Ltd
    Plant, cell & environment 16 (1993), S. 0 
    Details
    ISSN: 1365-3040
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Carbon transport across the plasma membrane, and carbon fixation were measured in perfused Chara internodal cells. These parameters were measured in external media of pH 5·5 and pH 8·5, where CO2 and HCO3- are, respectively, the predominant carbon species in both light and dark conditions. Cells perfused with medium containing ATP could utilize both CO2 and HCO3- from the external medium in the light. Photosynthetic carbon fixation activity was always higher at pH 5·5 than at pH 8·5. When cells were perfused either with medium containing hexokinase and 2-deoxyglucose to deplete ATP from the cytosol (HK medium) or with medium containing vanadate, a specific inhibitor of the plasma membrane H+-ATPase (V medium), photosynthetic carbon fixation was strongly inhibited at both pH 5·5 and 8·5. Perfusion of cells with medium containing pyruvate kinase and phosphoenolpyruvate (PEP) to maximally activate the H+-ATPase (PK medium), stimulated the photosynthetic carbon fixation activities. Oxygen evolution of isolated chloroplasts and the carbon fixation of cells supplied 14C intracellularly were not inhibited by perfusion media containing either hexokinase and 2-deoxyglucose or vanadate. The results indicate that Chara cells possess CO2 and HCO3- transport systems energized by ATP and sensitive to vanadate in the light. In the dark, intact cells also fix carbon. By contrast, in cells perfused with medium containing ATP, no carbon fixation was detected in 1 mol m -3 total dissolved inorganic carbon (TDIC) at pH 8·5. By increasing TDIC to 10 mol m-3, dark fixation became detectable, although it was still lower than that of intact cells at 1mol m-3 TDIC. Addition of PEP or PEP and PEP carboxylase to the perfusion media significantly increased the dark-carbon fixation. Perfusion with vanadate had no effect on the dark-carbon fixation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-3040.1993.tb00483.x
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  • 3
    Electronic Resource
    Electronic Resource
    Sequence diversity of Mhc genes in lake whitefish (2001)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of fish biology 58 (2001), S. 0 
    Details
    ISSN: 1095-8649
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The sequence variation of three exons of the major histocompatibility complex (Mhc) was examined in a lake whitefish Coregonus sp., population from the Swiss lake of Hallwil. DNA sequences from the Mhc class I A1, A2 and class II B1 exons, corresponding to the α1, α2 and β1 domains of the Mhc glycoproteins, were obtained by the polymerase chain reaction followed by cloning and sequencing. The numbers of variable sequences detected for each exon were 15 (A1), 11 (A2) and 20 (B1). Levels of nucleotide similarity ranged from 82 to 99% for the A1 exon, 58–96% for the A2 and 88–99% for the B1 exon. At the A1 and B1 exons, the nonsynonymous substitution rates (dn) exceeded synonymous substitution rates (ds) greatly within the peptide binding regions, indicating the effect of balancing selection. Sequence diversity at the A2 exon did not seem to be maintained by balancing selection (ds 〉 dn). Phylogenetic comparison of whitefish Mhc sequences with sequences from other salmonid species and more distantly related teleosts indicated shared ancestral (trans-species) polymorphism.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1095-8649.2001.tb02258.x
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  • 4
    Electronic Resource
    Electronic Resource
    Size of prey ingested by whitefish, Coregonus sp., larvae. Are Coregonus larvae gape-limited predators? (1990)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of fish biology 36 (1990), S. 0 
    Details
    ISSN: 1095-8649
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The feeding ecology of whitefish, Coregonus, larvae in oligotrophic Lake Sarnen and eutrophic Lake Hallwil is presented, taking into consideration the size of the prey ingested in relation to the mouth size of larvae and to the availability of zooplankton.When larvae grow from 10 to 16 mm their mouth width increases from 500 to 1000 μm while their gape height, with the mouth open at 45 and 90°, increases from 200 to 400 μm and from 400 to 700 μm, respectively, whichever lake is considered.The differences found in the mean prey width between the two lakes arise in part from the composition of diets, and in part from differences in size of the available prey organisms. Whatever the origin of the larvae and the taxon we consider, larvae seem to ingest the most abundant prey size without selecting the biggest ones available even if, from a mechanical point of view, they would be able to ingest them.Taking into account the generally low density of zooplankton in the uppermost layer of the lake, we conclude that whitefish larvae eat in the manner such as to decrease the cost of prey capture, in terms of energy and time, by choosing the more abundant prey available.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1095-8649.1990.tb03520.x
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  • 5
    Electronic Resource
    Electronic Resource
    Palaeomagnetic Results From the Tibetan Sedimentary Series of the Manang Area (North Central Nepal) (1991)
    Oxford, UK : Blackwell Publishing Ltd
    Geophysical journal international 104 (1991), S. 0 
    Details
    ISSN: 1365-246X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Geosciences
    Notes: The remanent magnetization of Palaeozoic and Mesozoic carbonates from the Tibetan Sedimentary Series (TSS) of the Manang area north of the Annapurna Range (north central Nepal) has been investigated. the TSS forms part of the tectonic unit between the Indian-Eurasian plate boundary in the north and the Main Central Thrust (MCT) in the south.Apart from a present field direction, three remanence components have been separated: a normal polarity, post-folding remanence (negative fold test) is carried by pyrrhotite (the core corrected mean direction for five sites with 47 specimens from Silurian/Devonian rocks is decl. = 312.9°, incl. = 58.3°α95= 17.6°, K= 19.8). the origin of this component is not understood.A more precisely determined, reversed polarity, post-folding remanence (99 per cent negative fold test) is also carried by pyrrhotite (the core corrected mean direction for 12 sites with 229 specimens from Carboniferous/Triassic rocks is decl. = 196.4°, incl. = -65.9°, α95= 3.2°, k = 183).One site of Lower Carinan age shows—in addition to the pyrrhotite component— a second, stable remanence direction carried by magnetite. the fold test is 99 per cent positive and there is a high probability that the remanence is of detrital origin (the bedding corrected direction for one site with 40 specimens is decl. =334.3°, incl. = -54.1°, α95= 4.2°, k = 30.2, palaeolatitude 34.6°S).The direction of the magnetite component coincides with other Triassic palaeodirection data from the literature and the inclination suggests a palaeoposition of the TSS at the northern margin of India at this time. the northern extent of India in the Triassic cannot be reconstructed from the data because of the strong anticlockwise rotation (48°) of stable India since this time.The inclination of the reverse polarity pyrrhotite component is 19° steeper than expected from the maximum northern position of the area. This suggests a regional northward tilt along the MCT which is supported by steep inclinations found in other areas.Declination data for the reverse polarity pyrrhotite component and for the magnetite component deviate clockwise from expected values for the area by 23°-34° (calculated from the Indian APWP). Utilizing Klootwijk, Conaghan & Powell's (1985) rotational underthrusting model, the minimum magnitude of continental underthrusting between the TSS and stable India at the longitude of central Nepal (84°E) has been calculated from the declination of our reverse polarity pyrrhotite component. A possible range of 520–1100 km results from consideration of different tectonic models.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-246X.1991.tb02510.x
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  • 6
    Electronic Resource
    Electronic Resource
    CO2 laser welding of polymers (1992)
    Stamford, Conn. [u.a.] : Wiley-Blackwell
    Polymer Engineering and Science 32 (1992), S. 582-585 
    Details
    ISSN: 0032-3888
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Deep penetration welding of polymers can be carried out at high speed with relatively low laser power. This results from an efficient coupling CO2 laser radiation to polymers that leads to volume heating. A brief review of energy coupling and heat transfer effects in polymers under CO2 laser welding conditions is given. Some examples of low power (10 to 100 watt) CO2 welding of polypropylene and polyethylene at depths of up to 1.5 cm are discussed.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/pen.760320903
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  • 7
    Electronic Resource
    Electronic Resource
    Mikrobielle Werkstoffzerstörung - Biofilm und Biofouling: Biofilme in industriellen Wasserkreisläufen. Fallbeispiel: Prozeßwassersystem einer Papierfabrik (1994)
    Weinheim [u.a.] : Wiley-Blackwell
    Materials and Corrosion/Werkstoffe und Korrosion 45 (1994), S. 65-67 
    Details
    ISSN: 0947-5117
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Microbial deterioration of materials - biofilm and biofouling: Biofilms in industrial water circuits. Case history: Process water system in a paper factory
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/maco.19940450113
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  • 8
    Electronic Resource
    Electronic Resource
    Ein alternativer Weg zur Darstellung von Bi2Sr2CaCu2Ox, YBa2Cu3O7-δ, und YBa2Cu3-xMxO7-δ (M = Ni, Ag und x ≤ 33 Mol%)Professor Rudolf Hoppe zum 70. Geburtstage gewidmet (1992)
    Weinheim : Wiley-Blackwell
    Zeitschrift für anorganische Chemie 616 (1992), S. 186-190 
    Details
    ISSN: 0044-2313
    Keywords: Preparation of quaternary copper oxides ; high Tc superconductors ; characterization by X-ray powder methods ; AC- and DC-susceptibility ; Chemistry ; Inorganic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: An Alternative Method for Preparation of Bi2Sr2CaCu3Ox, YBa2Cu3O7-δ, and YBa2Cu3-xMxO7-δ (M = Ni, Ag and x ≤ 33 Mol%)Oxidation of quenched melts of metals in a well defined argon/oxygen atmosphere has been found as an alternative method to prepare hight purity samples of Bi2Sr2CaCu2Ox without Pb-stabilization. A special equipment for the oxidation of powder samples will be described. By our new method it is also possible to prepare YBa2Cu3O7-δ and derivatives of this compound, where Cu is substituted by high amounts of Ni or Ag.
    Notes: Es wird eine alternative Präparationsmethode beschrieben, die es ermöglicht, durch Oxidation abgeschreckter Metallschmelzen in einer Sauerstoff-Argon-Strömungsapparatur sehr saubere, nicht Pb-stabilisierte Bi2Sr2CaCu2Ox-Präparate herzustellen. Dieser Weg ist auch für die Darstellung von YBa2Cu3O7-δ und Substitutionsderivaten dieser Verbindung mit hohen Konzentrationen an Ni oder Ag geeignet.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/zaac.19926161030
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  • 9
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    A new fungal large subunit ribosomal RNA primer for high-throughput sequencing surveys (2016)
    Oxford University Press
    Details
    Publication Date: 2016-01-31
    Description: The inclusion of phylogenetic metrics in community ecology has provided insights into important ecological processes, particularly when combined with high-throughput sequencing methods; however, these approaches have not been widely used in studies of fungal communities relative to other microbial groups. Two obstacles have been considered: (1) the internal transcribed spacer (ITS) region has limited utility for constructing phylogenies and (2) most PCR primers that target the large subunit (LSU) ribosomal unit generate amplicons that exceed current limits of high-throughput sequencing platforms. We designed and tested a PCR primer (LR22R) to target approximately 300–400 bp region of the D2 hypervariable region of the fungal LSU for use with the Illumina MiSeq platform. Both in silico and empirical analyses showed that the LR22R–LR3 pair captured a broad range of fungal taxonomic groups with a small fraction of non-fungal groups. Phylogenetic placement of publically available LSU D2 sequences showed broad agreement with taxonomic classification. Comparisons of the LSU D2 and the ITS2 ribosomal regions from environmental samples and known communities showed similar discriminatory abilities of the two primer sets. Together, these findings show that the LR22R–LR3 primer pair has utility for phylogenetic analyses of fungal communities using high-throughput sequencing methods.
    Print ISSN: 0168-6496
    Electronic ISSN: 1574-6941
    Topics: Biology
    Published by Oxford University Press
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  • 10
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    Two N-terminally truncated variants of human {beta}-galactoside {alpha}2,6 sialyltransferase I with distinct properties for in vitro protein glycosylation (2016)
    Oxford University Press
    Details
    Publication Date: 2016-10-20
    Description: Sialic acid groups of protein N -glycans are important determinants of biological activity. Exposed at the end of the glycan chain, they are potential targets for glycan remodeling. Sialyltransferases (STs; EC 2.4.99) are the enzymes that catalyze the sialic acid transfer from a CMP-activated donor on to a carbohydrate acceptor in vivo. Recombinant expression of the full-length human β-galactoside α2,6 sialyltransferase I (ST6Gal-I) was hampered and therefore variants with truncated N-termini were investigated. We report on the distinct properties of two N-terminally truncated versions of ST6Gal-I, namely 89ST6Gal-I and 108ST6Gal-I, which were successfully expressed in human embryonic kidney cells. The different properties of these enzymes result most probably from the loss of interactions from helix α1 in the 108ST6Gal-I variant, which plays a role in acceptor substrate binding. The K m for N -acetyl- d -lactosamine was 10-fold increased for 108ST6Gal-I (84 mM) as compared to 89ST6Gal-I (8.3 mM). The two enzyme variants constitute a suitable tool box for the terminal modification of N -glycans. While the enzyme 89ST6Gal-I exhibited both ST (di-sialylation) and sialidase activity on a monoclonal antibody, the enzyme 108ST6Gal-I showed only ST activity with specificity for mono-sialylation.
    Print ISSN: 0959-6658
    Electronic ISSN: 1460-2423
    Topics: Biology , Medicine
    Published by Oxford University Press
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