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  • Articles  (17)
  • Life Sciences  (17)
  • Wiley-Blackwell  (17)
  • American Chemical Society
  • American Institute of Physics (AIP)
  • De Gruyter
  • Oxford University Press
  • Society of Petroleum Engineers
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  • 2015-2019
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  • 1980-1984  (17)
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  • Chemistry and Pharmacology  (17)
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  • Articles  (17)
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  • Wiley-Blackwell  (17)
  • American Chemical Society
  • American Institute of Physics (AIP)
  • De Gruyter
  • Oxford University Press
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  • 1
    Electronic Resource
    Electronic Resource
    Analysis of human erythrocyte membrane vesicles produced by shearing (1980)
    New York, N.Y. : Wiley-Blackwell
    Journal of Supramolecular Structure 13 (1980), S. 1-13 
    Details
    ISSN: 0091-7419
    Keywords: human erythrocyte membranes ; membrane microvesicles ; sialoglycopeptides ; protein content of membranes ; enzymic content of membranes ; acetylcholinesterase ; Mg++-ATPase ; Na+ ; K+-ATPase ; NADH oxidoreductase ; GAPD of membranes ; Life Sciences ; Molecular Cell Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Shearing of ghosts in a French pressure cell produces three classes of microvesicles that differ from endocytic vacuoles, exocytic vacuoles, and inside-out vesicles. It was thought that an analysis of these vesicles might provide some clues about the assembly of proteins within the human erythrocyte membrane. The microvesicles were separated into three visible bands, labeled top, middle, and bottom, and assayed for activity of Mg++-ATPase, Na+, K+-ATPase, acetylcholinesterase, glyceraldehyde-phosphate dehydrogense, and NADH oxidoreductase. Their proteins were also characterized by polyacrylamide gel electrophoresis with both Coomassie blue staining, to assess total protein content and distribution, and PAS-staining, to characterize sialoglycopeptides. In order to minimize problems inherent in ghost preparation, Dodge or hypotonic ghosts and glycol or isotonic ghosts were used in all studies. Middle membrane vesicles most resembled intact ghosts. Top vesicles had reduced levels of NADH oxidoreductase and more PAS-2 at the expense of PAS-1. The bottom vesicle class was very much enriched with PAS-1 at the expense of PAS-2, and PAS-3 was completely absent. In addition bottom vesicles had highest NADH oxidoreductase activity but lowest activity of all the other enzymes measured. These vesicle classes could not have been produced by tangential shearing through the membrane, nor could radial shearing through a membrane in which all proteins were free to move laterally have accounted for the three discrete vesicle classes or for their different patterns of enzymes and proteins. The analysis of the microvesicles produced by shearing is most consistent with radial shearing through membranes where there may be fixed domains superimposed on the basic fluid-mosaic structure.
    Additional Material: 9 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jss.400130102
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  • 2
    Electronic Resource
    Electronic Resource
    Reversible phosphorylation of the membrane-bound acetylcholine receptor (1980)
    New York, N.Y. : Wiley-Blackwell
    Journal of Supramolecular Structure 14 (1980), S. 163-174 
    Details
    ISSN: 0091-7419
    Keywords: Life Sciences ; Molecular Cell Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Additional Material: 11 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jss.400140205
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  • 3
    Electronic Resource
    Electronic Resource
    Polypeptide growth factors: Some structural and mechanistic considerations (1980)
    New York, N.Y. : Wiley-Blackwell
    Journal of Supramolecular Structure 14 (1980), S. 183-199 
    Details
    ISSN: 0091-7419
    Keywords: primary and secondary hormones ; mitogenicity ; insulin ; insulin-like growth factor ; nerve growth factor ; relaxin ; epidermal growth factor ; receptor-mediated endocytosis ; lysomes ; hormone mechanisms ; Life Sciences ; Molecular Cell Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Polypeptide growth factors are substances that stimulate an increase in cell size and/or cell number during embryonic development. In some cases, they have a similar effect on tissues in the mature organism where they function as “maintenance” factors to sustain cell viability. While their profound impact on cell behavior is well recognized, their relationship to other regulators of cell function has remained generally ill-defined. However, the developing appreciation of their hormone-like behavior suggests that they may be conveniently grouped with many other endocrine agents to form a broader group of secondary hormones. The utility of the classification is illustrated by the insulin-related family of molecules. It also serves to emphasize the similarities in function shared by many of these substances including trophic stimulation and modulation of gene expression. Internalization, though, appears to be another common feature. However, whether the uptake of the growth factor mediates an intracellular action or is designed solely to regulate responsiveness at the cell surface and/or degradation remains an important unanswered question. A brief review of two growth factors (nerve growth factor and epidermal growth factor) serves to outline the possible functions that may be served by this endocytotic process.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jss.400140207
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  • 4
    Electronic Resource
    Electronic Resource
    Specific protein phosphorylation during cyclic AMP-mediated morphological reversion of transformed cells (1980)
    New York, N.Y. : Wiley-Blackwell
    Journal of Supramolecular Structure 14 (1980), S. 241-254 
    Details
    ISSN: 0091-7419
    Keywords: cytoskeletons ; cell growth ; protein kinase ; morphology ; cyclic AMP ; phosphorylation ; transformation ; Life Sciences ; Molecular Cell Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Treatment of transformed Chinese hamster ovary cells with dibutyryl cAMP or other agents that elevate cAMP results in the acquisition of growth and morphology characteristic of normal fibroblasts. The role of specific protein phosphorylation in this process of morphological reversion has been examined using metabolic labelling of Chinese hamster ovary (CHO) cells with 32P-orthophosphate in the presence or absence of N6O2′-dibutyryladenosine 3′:5′-cyclic monophosphoric acid (Bt2cAMP). Analysis of labelled cultures by SDS gel electrophoresis and radioautography demonstrate dramatic changes in the phosphorylation of only 2 cellular proteins during reverse transformation. A 55,000 dalton protein (pp55) was phosphorylated and a 20,000 dalton protein (pp20) was dephosphorylated. The time course of these events was consistent with the kinetics of morphological reversion. The lower molecular weight species, pp20, was dephosphorylated within 15-30 minutes, prior to all morphological changes except membrane tranquilization. The higher molecular weight protein, pp55, was maximally phosphorylated over 1-2 hours following addition of Bt2cAMP, paralleling early stages in the establishment of fibroblastic form. The phosphorylated forms of pp20 and pp55 were both extracted from cellular cytoskeletons by 0.5% Triton X-100, but analysis of 35S-methioninelabelled cultures suggested that unphosphorylated pp 20 may be bound to the cytoskeleton. Since pp20 was found to comigrate with the 20,000 dalton myosin light chain, it is possible that dephosphorylation of CHO cell myosin induced by cAMP may alter its interaction with actin microfilaments and modulate the assembly of stress fibers during morphological reversion.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jss.400140213
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  • 5
    Electronic Resource
    Electronic Resource
    Direct linkage of EGF to its receptor: Characterization and biological relevance (1980)
    New York, N.Y. : Wiley-Blackwell
    Journal of Supramolecular Structure 14 (1980), S. 441-459 
    Details
    ISSN: 0091-7419
    Keywords: EGF receptors ; biotinyl EGF ; covalent EGF-receptor complexes - and 3T3 cell growth regulation ; on human placental membranes ; on cultured cells ; Life Sciences ; Molecular Cell Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: A small portion of the 125I-EGF that binds specifically to intact cells or isolated membranes from a variety of sources becomes directly and irreversibly linked to EGF receptors. This provides a simple technique for affinity labeling the EGF receptor. Membranes isolated from the human epidermoid carcinoma cell line A431, which posesses extraordinarily high numbers of EGF receptors, gave rise to three major direct linkage complexes of MW = 160,000, 145,000, and 115,000. The time course for formation of each is similar, showing that 125I-EGF can form direct linkage complexes with several preexisting forms of the EGF receptor. The direct linkage of EGF to receptor is slow in comparison to 125I-EGF binding, but both processes have similar susceptibilities to competition by unlabeled EGF.EGF was modified chemically with the amino site-specific reagent, N-hydroxysuccinimidyl biotin. The biotinyl-EGF had a reduced capacity to engage in direct linkage complex formation with no concomitant reduction in its ability to bind to EGF receptors. Since native and biotinyl EGF have identical abilities to stimulate the uptake of 3H-thymidine into DNA when incubated with cultured murine 3T3 cells, the direct linkage of EGF to its receptor does not appear to play an important role in EGF-stimulated mitogenesis.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jss.400140404
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  • 6
    Electronic Resource
    Electronic Resource
    Purification of human interleukin 1 (1980)
    New York, N.Y. : Wiley-Blackwell
    Journal of Supramolecular Structure 13 (1980), S. 457-466 
    Details
    ISSN: 0091-7419
    Keywords: lymphocyte activating factor (LAF) ; Interleukin I ; purification of human IL-1 ; hollow fiber diafiltration ; isoelectric focusing ; polyacrylamide gel ; electrophoresis ; human monocytes ; endotoxin stimulation ; IL-1 release ; thymocyte mitogenic activity ; Life Sciences ; Molecular Cell Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Interleukin I (IL-1) is a lymphocyte stimulant released by human monocytes cultured for 18-24 hours in tissue culture medium containing 5% serum and the non-specific immunostimulant lipopolysaccharide (LPS). Human IL-1 is found in the conditioned medium in a low molecular weight (∼ 13,000) and a high molecular weight (∼ 85,000) form. The high MW activity may result from the formation of a complex between IL-1 and serum constituents. During the course of purification, the low MW IL-1 activity is often recovered in a high MW form. Hollow fiber diafiltration and membrane ultrafiltration has been found to rapidly separate low MW IL-1 from all measurable protein with a yield of 4% of the original activity. The IL-1 which converts to the high MW form during the purification is recoverable, 21% of the original activity, but contains small amounts of serum proteins. Isoelectric focusing (IEF) of the low MW IL-1 resulted in a very highly purified sample which was analyzed by polyacrylamide gel electrophoresis (PAGE). Utilizing a new staining procedure which detects less than 1 ng of protein per band, the IEF-purified IL-1 revealed trace quantities ( 〈 1 ng) of a slowly migrating protein similar to immunoglobulin and no other bands. There were no bands which corresponded with the known electrophoretic mobility of IL-1. Since the samples applied to the gel contained significant biological activity, this result implies that human IL-1 is biologically active in picogram quantities.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jss.400130405
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  • 7
    Electronic Resource
    Electronic Resource
    Steroid hormone receptor studies in melanoma model systems (1980)
    New York, N.Y. : Wiley-Blackwell
    Journal of Supramolecular Structure 13 (1980), S. 35-46 
    Details
    ISSN: 0091-7419
    Keywords: estrogen receptor ; glucocorticoid receptor ; estradiol ; diethylstilbestrol ; dexamethasone ; B-16 mouse melanoma ; Syrian hamster melanoma ; Life Sciences ; Molecular Cell Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: The Transplantable B-16 melanotic melanoma carried in syngeneic C57B1/6J female mice and the Syrian hamster melanoma cell line, RPMI 3460, were utilized to determine whether steroid-hormone receptors are present in animal melanomas. In the B-16 melanoma, a cytoplasmic-estrogen receptor is detectable, but there is no evidence for androgen or progestin receptors. Some tumors contain a glucocorticoid-binding macromolecule. Sucrosedensity gradient centrifugation of cytosol after incubation with [3H]-estradiol revealed an 8S peak that was suppressed by excess radioinert diethylstilbesterol. Binding varied from 5-35 fmoles per mg cytosol protein. Scatchard analysis of [3H]-estradiol binding in cytosol yielded a single class of high-affinity binding sites; the dissociation constant is 6 × 10-10 M. The receptor molecule is shown to be estrogen-specific by ligand competition assays. In contrast to B-16 melanoma, no estrogen, androgen, or progestin receptor can be found in the Syrian hamster melanoma cell line. However, a substantial level of specific binding is observed using [3H]-dexamethasone. Sucrose-gradient centrifugation of cytosol from this cell line after incubation with [3H]-dexamethasone revealed a 7S peak that was suppressed by excess radioinert dexamethasone. Scatchard analysis indicated a single class of high affinity sites with a dissociation constant of 2 × 10-9 M. Binding levels from 70-610 fmoles per mg cytosol protein were observed. The Syrian hamster melanoma cells also exhibit a biological response to glucocorticoids: Dexamethasone causes both an inhibition of growth and a decrease in final-cell density in these cells.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jss.400130104
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  • 8
    Electronic Resource
    Electronic Resource
    Induction and long-term maintenance of Thy-1 positive T lymphocytes: Derivation from continuous bone marrow cultures (1980)
    New York, N.Y. : Wiley-Blackwell
    Journal of Supramolecular Structure 13 (1980), S. 533-539 
    Details
    ISSN: 0091-7419
    Keywords: T lymphocytes ; TCGF ; continuous marrow cultures ; Life Sciences ; Molecular Cell Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: In the present study we investigated the presence of T-lymphocyte progenitors in the long-term murine bone marrow culture system described by Dexter: mature Thy-1 antigen-bearing T lymphocytes are lost in these cultures after a few days. By culturing nonadherent cells from such cultures in the presence of a supernatant of concanavalin A-stimulated spleen cells, a source of T-cell growth factor, we found that Thy-1 positive blast cells proliferated together with a second population of Thy-1 negative cells. These two populations of cells have been maintained in long-term in vitro cultures by passaging the cells in fresh conditioned medium at regular intervals. Moreover, we have been able to establish pure cultures of the Thy-1-bearing blast cells after separating them from the non-T cells using their adherence property to plastic surfaces. Long-term cultures of T lymphocytes can thus be established from long-term marrow cultures as well as from the spleen, thymus or fresh bone marrow.
    Additional Material: 2 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jss.400130412
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  • 9
    Electronic Resource
    Electronic Resource
    A comparative dielectric study of human serum low density lipoprotein before and after partial digestion by trypsin (1980)
    New York, N.Y. : Wiley-Blackwell
    Journal of Supramolecular Structure 13 (1980), S. 47-52 
    Details
    ISSN: 0091-7419
    Keywords: lipoprotein ; trypsin ; dielectric measurements ; counterions ; α-dispersion ; Life Sciences ; Molecular Cell Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: The relative permittivity of aqueous solutions of human serum low density lipoprotein (LDL) and partially trypsin digested lipoprotein (T-LDL) has been determined for various concentrations at 20°C over the frequency range 0.15-100 MHz. Comparison of the dielectric dispersion curves for the digested lipoprotein with those for the native preparation revealed a larger low-frequency dielectric increment, which may be attributed to an increase in the number of counterions moving over the surface of the molecule. An explanation of this observation is an elevation of 70% in the net negative charge on the surface of the trypsin-treated particle as compared to its native counterpart.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jss.400130105
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  • 10
    Electronic Resource
    Electronic Resource
    Properties of receptors for epidermal growth factor in detergent solution: Evidence for heterogeneous aggregated states (1980)
    New York, N.Y. : Wiley-Blackwell
    Journal of Supramolecular Structure 14 (1980), S. 511-525 
    Details
    ISSN: 0091-7419
    Keywords: EGF receptors - solubilization ; aggregates in nonionic detergent ; gel filtration chromatography ; zonal sedimentation ; on A431 membranes ; Life Sciences ; Molecular Cell Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Between 60% and 100% of epidermal growth factor (EGF) binding activity was recovered from membranes of the A431 human epidermoid carcinoma cell line treated with solutions containing the nonionic detergent Triton X-100. Approximately half of the recovered binding activity was sedimented at low centrifugal forece and hence was operationally insoluble in nonionic detergent solution. Receptors in both the detergent-soluble and -insoluble fractions displayed similar affinities for 125I-EGF, and the values were in good agreement with those obtained for receptors in untreated membranes. The receptors in both fractions also formed identical direct linkage complexes with 125I-EGF in similar yield, providing no evidence for partitioning of different molecular species of EGF receptors in the detergent-soluble and -insoluble fractions.Gel chromatography of the detergent-soluble membrane fraction on Sepharose 6-B revealed heterogeneity of 125I-EGF binding activity; the smallest and most monodisperse peak of activity resolved by this technique was eluted at a Stokes radius of 95 Å. Operationally soluble 125I-EGF binding activity also behaved heterogeneously during velocity sedimentation; more than half the activity sedimented more rapidly than the apparently monidisperse, 7S form. An average of less than half the nonionic detergent-solubilized activity recovered from 10 independent membrane preparations behaved as an apparently monodisperse entity. Since a maximum of 60% of 125I-EGF binding activity was operationally soluble, less than 25% of the total EGF binding activity was recovered in an apparently monodisperse form. The remaining 75% of the EGF receptors displayed a marked tendency to exist as aggregates in nonionic detergent solutions.
    Additional Material: 9 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jss.400140409
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