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  • ADMS  (1)
  • Antibody  (1)
  • Springer  (2)
  • American Society of Hematology
  • 2015-2019
  • 2010-2014
  • 2000-2004  (1)
  • 1995-1999  (1)
Collection
Publisher
  • Springer  (2)
  • American Society of Hematology
Years
  • 2015-2019
  • 2010-2014
  • 2000-2004  (1)
  • 1995-1999  (1)
Year
  • 1
    ISSN: 1573-0603
    Keywords: Adhesin ; Antibody ; Bacteria ; Fab ; Phage display
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Surface proteins provide a multitude of functions for the bacterial cell. Antibodies to these proteins can provide tools for tagging bacteria and characterizing protein function. Phage display technology has emerged as a powerful method for producing monoclonal Fabs in Escherichia coli. In an effort to study the adhesion mechanisms of Streptococcus parasanguis FW213, Fabs specific for the surface adhesin protein Fap1 were produced using phage display. The immune repertoire of a mouse injected with purified Fap1 was cloned into the phagemid vector pCOMB3, and a combinatorial Fab library was expressed in E. coli. A cell-based panning method using whole S. parasanguis cells was developed and has been shown to be a means for enriching for Fabs specific for the Fap1 protein.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1573-2959
    Keywords: PM10 ; urban air quality ; background ; NAME ; ADMS ; long range transport ; air pollution ; particulates ; nitrogen dioxide ; sulphur dioxide
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract Norwich is the eastern most city in the United Kingdom. Despite a population of only 100,000 and very little local industry, studies have shown that the city experiences levels of nitrogen dioxide, ozone, particulates and sulphur dioxide exceeding the UK Air Quality Standards. Because of Norwich's situation within a large, predominantly rural area a large non-resident workforce is one factor that contributes to large, often very congested traffic flows. The city's location close to the European mainland also exposes it to polluted airmasses transported from the continent, especially in the case of particulates and ozone. In order to assess the relative contributions of local and regional sources, data from rural and urban monitoring sites are to be used in conjunction with ADMS-Urban and the UK Meteorological Office's NAME model.
    Type of Medium: Electronic Resource
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