ALBERT

Description: Water sampling was carried out weekly between April and October 2016. Water samples were taken from two shallow sites (less than 5 km apart) in the Orkneys. Sampling was done using a bucket at both sampling locations. Water for filtration was transported to the Orkney Lobster Hatchery where 500–1000 mL of water was filtered over 0.4 µm polycarbonate (PC) filter (Whatman, 47mm). Filters were stored at -20 °C until DNA isolation in the laboratory. The samples were processed at the AWI to produce a metabarcoding data set with the goal of analysing the general diversity at the two sites but also to investigate the dynamics of harmful algal bloom species (HABs). As the data also contained a wealth of information on protistan parasites they were used to produce an additional manuscript on oomycete infections in the planktonic foodweb at the two sites. The bioinformatic processing of the raw sequence files was conducted as follows. The low-quality 3'-ends of the reads were trimmed by Trimmomatic (version 0.38) and the paired-ends were merged by VSEARCH (version 2.3.0). Cutadapt (version 1.19, was used to adjust the sequence orientation and to remove the forward and reverse primer matching sequence segments. Sequences were only kept in the sequence pool if both primer matching segments could be detected. To ensure a high sequence quality the remaining sequences were filtered by VSEARCH and only kept if the expected base error (sum of all base error probabilities) of a sequence was above 0.25. Chimeric sequences were predicted sample-wise by VSEARCH in de novo mode with default settings and removed from the sequence pool as well. Only samples which consisted of at least 10000 sequences after filtering were considered for further analyses. The remaining sequences were clustered into OTUs by the tool swarm (version 2.2.2) with default settings. For each OTU the most abundant amplicon was selected as representative and taxonomically annotated with the default classifier implemented in mothur (version 1.38.1). As reference the Protist Ribosomal Reference database (PR2), version 4.10 was chosen and the minimum confidence cut-off for annotation was set to a value of 80. Further details of the pipeline are provided in (Sprong, et al. 2020; doi:10.1016/j.seares.2020.101914).

Description: Water samples were collected from the long-term ecological research (LTER) site at Helgoland and the eukaryotic microbial community was assessed. 18S V4 region was amplified using the primer set 528iF /964iR and amplicon sequencing was performed on an Illumina MiSeq™ sequencer in a 2 × 300 bp paired-end run. Sequence data have been deposited in the European Nucleotide Archive (ENA) at the European Bioinformatics Institute (EMBL-EBI) under accession number PRJEB37135 using the data brokerage service of the German Federation for Biological Data (GFBio). 21 million sequences remained after bioinformatic processing and were clustered into Operational Taxonomic Units (OTUs). The Protist Ribosomal Reference database (PR2), version 4.11.1 was used as reference database.

Description: Raw data acquired by two thermosalinographs (SBE21, SeaBird GmbH) on board RV Polarstern were processed to yield a calibrated and validated data set of temperature, conductivity and salinity during expedition PS121. Both sensors were equipped with a more accurate external temperature sensor (SBE38, Sea-Bird GmbH). Data were downloaded from the DAVIS SHIP data base (https://dship.awi.de) with a resolution of 1 sec. The raw hex data were converted to temperature and conductivity while a sensor drift correction was applied using calibration coefficients from before and after the expedition. Salinity was calculated according to the instructions from the Practical Salinity Scale PSS-78, using the obtained (internal) temperature and conductivity data and a pressure of 11 dbar which represents the water depth of the inlet of the TSG system on Polarstern. A speed filter of 0.5 knots minimum speed was applied. Processed data are provided as 10min means of seawater salinity, conductivity and temperature, aligned with position data taken from the master track of PS121 (doi:10.1594/PANGAEA.908157). Further details and evaluation of the data is outlined in the data processing report found at the EPIC repository under URL (https://hdl.handle.net/10013/epic.7fffb528-06bd-48ae-8489-cea0444c4eab).

Description: Time series data of physical oceanography (seawater conductivity, temperature, pressure, salinity), ocean current velocities and hydroacoustics were obtained from mooring F4-18 in the Fram Strait in July 2018 - August 2019 as part of the Helmholtz infrastructure program Frontiers in Arctic Marine Monitoring (FRAM) and the long-term monitoring program at AWI HAUSGARTEN. The mooring was deployed during Polarstern expedition PS114, and recovered during PS121. The attached archive contains raw data files of 6 Seabird SBE37 microcats (nominal depths: 63m, 150m, 250m, 500m, 731m, 1202m; sampling interval 1h), one RDI Workhorse Longranger ADCP (depth: 380m; sampling interval: 1h) and two AADI RCM11 current meters (depths: 730m, 1208m; sampling interval: 2h). The mooring also included a Develogic Sonovault (nominal depth: 800m; data archived elsewhere). Auxiliary information such as sensor calibration sheets, mooring diagrams and schedule files are also provided, if applicable.

Description: Time-series data of physical oceanography and carbon/particle export were obtained from mooring HG-N-FEVI-37 in the Fram Strait in July 2018 - August 2019 as part of the Helmholtz infrastructure program Frontiers in Arctic Marine Monitoring (FRAM) and the long-term monitoring program at AWI HAUSGARTEN. The mooring was deployed during RV POLARSTERN expedition PS114, and recovered during PS121. The attached archive contains raw data files of two Seabird SBE37 microcats (nominal depths: 48m, 2626m; sampling interval 1h), two AADI RCM11 current meters (nominal depths: 2448m, 2624m; sampling interval 1h) and one AADI Seaguard current meter (nominal depth: 210m; sampling interval 1h). The mooring also included two sediment traps (nominal depths: 203m, 2441m). Auxiliary information such as sensor calibration sheets, mooring diagrams and schedule files are also provided, if applicable.

Description: Time-series data of physical oceanography, nutrient biogeochemistry, molecular biology and carbon/particle export were obtained from mooring F4-S-3 in the Fram Strait in July 2018 - August 2019 as part of the Helmholtz infrastructure program Frontiers in Arctic Marine Monitoring (FRAM) and the long-term monitoring program at AWI HAUSGARTEN. The mooring was deployed during RV POLARSTERN expedition PS121, and recovered during PS126. The attached archive contains raw data files of two Seabird SBE37 microcats (nominal depths: 16m, 21m, 47m; sampling interval 1h), one Seabird SBE56 temperature logger (nominal depth: 38m; sampling interval: 30s), one Wetlabs ECO PAR sensor (nominal depth: 21m; sampling interval 2h), one Wetlabs ECO Triplet fluorometer (nominal depth: 21m; sampling interval 2h), one Satlantics SUNA nitrate sensor (nominal depth: 21m; sampling interval 6h), one Sunburst SAMI-pCO2 sensor (nominal depth: 21m; sampling interval 2h) and one Sunburst SAMI-pH sensor (nominal depth: 21m; sampling interval 3h). The mooring also included a McLane RAS water sampler (nominal depth: 21m), a McLane PPS phytoplankton sampler (nominal depth: 23m) and two sediment traps (nominal depths: 204m, 613m). Auxiliary information such as sensor calibration sheets, mooring diagrams and schedule files are also provided, if applicable.

Description: Time-series data of physical oceanography, ocean current velocities and carbon/particle export were obtained from mooring HG-IV-FEVI-40 in the Fram Strait in August 2019 - June 2021 as part of the Helmholtz infrastructure program Frontiers in Arctic Marine Monitoring (FRAM) and the long-term monitoring program at AWI HAUSGARTEN. The mooring was deployed during RV POLARSTERN expedition PS121, and recovered during PS126. The attached archive contains raw data files of five Seabird SBE37 microcats (nominal depths: 79m, 257m, 404m, 1204m, 2530m; sampling interval 1h), four Seabird SBE56 temperature loggers (nominal depths: 104m, 154m, 204m, 329m; sampling interval 30s), one Nortek Aquadopp current meter (nominal depth: 754m; sampling interval 20 min), one RDI Longranger ADCP (nominal depth: 413m; sampling interval 1h) and four AADI Seaguard current meters (nominal depths: 207m, 1234m, 2352m, 2528m; sampling interval 1h). The mooring also included three sediment traps (nominal depths: 200m, 1227m, 2345m) and one BioOptical Platform (nominal depth: 569m). Auxiliary information such as sensor calibration sheets, mooring diagrams and schedule files are also provided, if applicable.