ALBERT

Description: Insects can play an important role to upgrade waste streams into high-grade proteins and fats as food and feed ingredients or non-food products. The aim of this research was to assess the feasibility to use waste streams with a low value for direct application as animal feed as substrates to grow BSF larvae in terms of larval growth rate, waste reduction index, and efficiency of conversion of ingested feed. The growth of black soldier fly (BSF), Hermetia illucens larvae and conversion of biowaste was assessed in triplicate in biowaste substrates: chicken feed (CF; reference diet), pig manure solid (PMS), Betafert® solid (BTFS), swill (SW), olive pulp (OP), pig manure liquid mixed with chicken feed (PMLCF), and silage grass (SG). Per kilogram fresh substrate 2500 starter (8-days-old, second instar) larvae were incubated in 21 plastic containers (75 × 47 × 15 cm). The BSF larvae were fed according to a batch feeding system. Highest growth rate was found in larvae reared on SW (13.4 mg/d). Larval growth rate was even higher than in larvae reared on the reference substrate CF (7.2 mg/d). Growth rate in larvae reared on PMLCF (7.3 mg/d) did not differ from CF, whereas growth rate of larvae reared on PMS (3.2 mg/d) was lower than on CF. Growth rate of larvae reared on BTFS, OP and SG was very low (0.6, 0.2 and 0.7 mg/d, respectively). Waste Reduction Index (WRI) was highest on SW (11.3), followed by PMLCF (9.3), and both were higher than WRI on CF (8.5). Waste Reduction Index further decreased in descending order from PMS, SG, BTFS to OP (7.6, 4.0, 2.9 and 1.7, respectively). The Efficiency of Conversion of Ingested substrate (ECI) was highest on SW (0.31), followed in descending order by PMLCF, CF and PMS (0.25, 0.21 and 0.18, respectively). The substrates OP, BTFS and SG (0.16, 0.15 and 0.14, respectively) resulted in a lower ECI than other substrates. Highest CO2 and lowest NH3 concentrations were found above substrates with the highest larval growth performances. This study showed that BSF larvae can be reared on different biowaste substrates; the growth rate of the larvae was extremely high on SW. The effects of chemical composition and physical properties of the substrates on larval growth and gas emissions should be further considered.

Description: Ports form a vital link in the global maritime supply chain, and adherence to the UN sustainable goals in each port development and operation is all-important. Improving the sustainability performance of port infrastructure requires identifying all relevant aspects of sustainability, defining suitable performance measures, applying tools for quantification, and proposing intervention measures if needed. We present a framework for assessing and managing the sustainability performance of port infrastructure incorporating these elements. We apply the framework to quay walls in the Port of Rotterdam (PoR); however, the information to assess only four out of fourteen sustainability themes was available. This fact underlines the need for sustainability monitoring and reporting. We also present a case study of a quay wall in PoR. Based on the vision of PoR and literature, the sustainability theme ‘air pollutants’ was given priority, the selected performance measure being the Greenhouse Gas (GHG) emissions. Dubocalc was chosen as a quantification tool and estimated a total emission of 1.9 kt of CO2eq for a 100-m length of quay wall over a 100-year lifecycle. The measures proposed to achieve climate neutrality in 2050 include using renewable energy for the Impressed Current Cathodic Protection and the temporary drainage systems, electrified transport and machinery, hydrogen as dredging fuel, and steel with hydrogen as a reduction-agent in the future. These could result in a total GHG reduction of 86% by the year 2050.

Description: Arterial media calcification (AMC) is predominantly regulated by vascular smooth muscle cells (VSMCs), which transdifferentiate into pro-calcifying cells. In contrast, there is little evidence for endothelial cells playing a role in the disease. The current study investigates cellular functioning and molecular pathways underlying AMC, respectively by, an ex vivo isometric organ bath set-up to explore the interaction between VSMCs and ECs and quantitative proteomics followed by functional pathway interpretation. AMC development, which was induced in mice by dietary warfarin administration, was proved by positive Von Kossa staining and a significantly increased calcium content in the aorta compared to that of control mice. The ex vivo organ bath set-up showed calcified aortic segments to be significantly more sensitive to phenylephrine induced contraction, compared to control segments. This, together with the fact that calcified segments as compared to control segments, showed a significantly smaller contraction in the absence of extracellular calcium, argues for a reduced basal NO production in the calcified segments. Moreover, proteomic data revealed a reduced eNOS activation to be part of the vascular calcification process. In summary, this study identifies a poor endothelial function, next to classic pro-calcifying stimuli, as a possible initiator of arterial calcification.

Description: Knowledge of phylogenetic relatedness and cytogenetic characteristics can facilitate breeding programs and interspecific hybridization in ornamentals. In this study genetic relationships, genome sizes and chromosome numbers were determined in a collection of 82 lavender genotypes, including 15 hybrids. Amplified Fragment Length Polymorphism (AFLP) marker analysis revealed 5 clusters, corresponding to the sections Lavandula, Stoechas, Dentatae, Pterostoechas and Subnudae. Genome sizes varied between 0.76 ± 0.02 pg 2C−1 and 4.80 ± 0.06 pg 2C−1 and chromosome numbers ranged from 2n = 22 to 2n = 100. Genome sizes of hybrids were intermediate to the progenitors genome sizes. All analyzed hybrid genotypes were characterized by even chromosome numbers, with 2n = 48 or 50. Using Principal Coordinate Analysis (PCoA) separate hybrid clusters were identified between the progenitor species clusters. Polyploidy and/or aneuploidy was observed in two hybrids genotypes: L. × intermedia ‘Heavenly Angel’ (2n = 100) and L. × heterophylla ‘Big Boy James’ (2n = 66). Results obtained in this study can be used to predict cross compatibility among different species, thus enabling targeted breeding towards the creation of new improved Lavandula cultivars.

Description: The precipitation of calcium carbonate is well studied in many fields of research and industry. Despite the fact that, or perhaps because of the fact that, it is well studied in many fields, different approaches have been used to describe the kinetics of the precipitation process. The aim of this study was to collect and compare the data available in the literature and find a consistent method to describe the kinetics of growth and nucleation of the various polymorphs of calcium carbonate. Inventory of the available data showed that a significant number of the literature sources were incomplete in providing the required information to recalculate the kinetic constants. Using a unified method, we obtained a unique set of parameters to describe the kinetics for growth for calcite, vaterite and amorphous calcium carbonate (ACC) and nucleation for vaterite and ACC. Recalculation of the kinetic constants demonstrated that calcite confirmed there are two growth mechanisms within one polymorph, namely pure spiral growth and spiral growth mixed with surface nucleation. The spiral growth does not show second-order growth, which is typically attributed to it. Re-evaluation of the available nucleation data confirmed the suggested existence of a second pure ACC polymorph with a solubility product between 10−5.87 and 10−5.51 mol2 kgw−2.

Description: Faced with the biggest virus outbreak in a century, world governments at the start of 2020 took unprecedented measures to protect their healthcare systems from being overwhelmed in the light of the COVID-19 pandemic. International travel was halted and lockdowns were imposed. Many nations adopted measures to stop the transmission of the virus, such as imposing the wearing of face masks, social distancing, and limits on social gatherings. Technology was quickly developed for mobile phones, allowing governments to track people’s movements concerning locations of the virus (both people and places). These are called contact tracing applications. Contact tracing applications raise serious privacy and security concerns. Within Europe, two systems evolved: a centralised system, which calculates risk on a central server, and a decentralised system, which calculates risk on the users’ handset. This study examined both systems from a threat perspective to design a framework that enables privacy and security for contact tracing applications. Such a framework is helpful for App developers. The study found that even though both systems comply with the General Data Protection Regulation (GDPR), Europe’s privacy legislation, the centralised system suffers from severe risks against the threats identified. Experiments, research, and reviews tested the decentralised system in various settings but found that it performs better but still suffers from inherent shortcomings. User tracking and re-identification are possible, especially when users report themselves as infected. Based on these data, the study identified and validated a framework that enables privacy and security. The study also found that the current implementations using the decentralised Google/Apple API do not comply with the framework.

Description: Effective gas dispersion and liquid mixing are significant parameters in the design of an inert-particle spouted-bed reactor (IPSBR) system. Solid particles can be used to ensure good mixing and an efficient rate of mass and heat transfer between the gas and liquid. In this study, computational fluid dynamics (CFD) coupled with the discrete phase model (DPM) were developed to investigate the effect of the feed gas velocity (0.5–1.5 m/s), orifice diameter (0.001–0.005 m), gas head (0.15–0.35 m), particle diameter (0.009–0.0225 m), and mixing-particle-to-reactor-volume fraction (2.0–10.0 vol.%) on the solid mass concentration, average solid velocity, and average solid volume fraction in the upper, middle, and conical regions of the reactor. Statistical analysis was performed using a second-order response surface methodology (RSM) with central composite design (CCD) to obtain the optimal operating conditions. Selected parameters were optimized to maximize the responses in the middle and upper regions, and minimize them in the conical region. Such conditions produced a high interfacial area and fewer dead zones owing to good particle dispersion. The optimal process variables were feed gas velocity of 1.5 m/s, orifice diameter of 0.001 m, gas head of 0.2025 m, a particle diameter of 0.01 m, and a particle load of 0.02 kg. The minimum average air velocity and maximum air volume fraction were observed under the same operating conditions. This confirmed the novelty of the reactor, which could work at a high feed gas velocity while maintaining a high residence time and gas volume fraction.

Description: Ursolic acid (UA), a pentacyclic triterpenoid extracted from various plants, inhibits cell growth, metastasis, and tumorigenesis in various cancers. Chemotherapy resistance and the side effects of paclitaxel (PTX), a traditional chemotherapy reagent, have limited the curative effect of PTX in esophageal cancer. In this study, we investigate whether UA promotes the anti-tumor effect of PTX and explore the underlying mechanism of their combined effect in esophageal squamous cell carcinoma (ESCC). Combination treatment with UA and PTX inhibited cell proliferation and cell growth more effectively than either treatment alone by inducing more significant apoptosis, as indicated by increased sub-G1 phase distribution and protein levels of cleaved-PARP and cleaved caspase-9. Similar to the cell growth suppressive effect, the combination of UA and PTX significantly inhibited cell migration by targeting uPA, MMP-9, and E-cadherin in ESCC cells. In addition, combination treatment with UA and PTX significantly activated p-GSK-3β and suppressed the activation of Akt and FOXM1 in ESCC cells. Those effects were enhanced by the Akt inhibitor LY2940002 and inverted by the Akt agonist SC79. In an in vivo evaluation of a murine xenograft model of esophageal cancer, combination treatment with UA and PTX suppressed tumor growth significantly better than UA or PTX treatment alone. Thus, UA effectively potentiates the anti-tumor efficacy of PTX by targeting the Akt/FOXM1 cascade since combination treatment shows significantly more anti-tumor potential than PTX alone both in vitro and in vivo. Combination treatment with UA and PTX could be a new strategy for curing esophageal cancer patients.

Description: In this paper, a robust observer-based control strategy for n-DOF uncertain robot manipulators with fixed-time stability was developed. The novel fixed-time nonsingular sliding mode surface enables control errors to converge to the equilibrium point quickly within fixed time without singularity. The development of the novel fixed-time disturbance observer based on a uniform robust exact differentiator also allows uncertain terms and exterior disturbances to be proactively addressed. The designed observer can accurately approximate uncertain terms within a fixed time and contribute to significant chattering reduction in the traditional sliding mode control. A robust observer-based control strategy was formulated, according to a combination of the fixed-time nonsingular terminal sliding mode control method and the designed observer, to yield global fixed time stability for n-DOF uncertain robot manipulators. The proposed controller proved definitively that it was able to obtain global stabilization in fixed time. The approximation capability of the proposed observer, the convergence of the proposed sliding surface, and the effectiveness of the proposed control strategy in fixed time were fully confirmed by simulation performance on an industrial robot manipulator.

Description: Traumatic brain injury (TBI) is the leading cause of death in young individuals, and is a major health concern that often leads to long-lasting complications. However, the electrophysiological events that occur immediately after traumatic brain injury, and may underlie impact outcomes, have not been fully elucidated. To investigate the electrophysiological events that immediately follow traumatic brain injury, a weight-drop model of traumatic brain injury was used in rats pre-implanted with epidural and intracerebral electrodes. Electrophysiological (near-direct current) recordings and simultaneous alternating current recordings of brain activity were started within seconds following impact. Cortical spreading depolarization (SD) and SD-induced spreading depression occurred in approximately 50% of mild and severe impacts. SD was recorded within three minutes after injury in either one or both brain hemispheres. Electrographic seizures were rare. While both TBI- and electrically induced SDs resulted in elevated oxidative stress, TBI-exposed brains showed a reduced antioxidant defense. In severe TBI, brainstem SD could be recorded in addition to cortical SD, but this did not lead to the death of the animals. Severe impact, however, led to immediate death in 24% of animals, and was electrocorticographically characterized by non-spreading depression (NSD) of activity followed by terminal SD in both cortex and brainstem.