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  • Competitiveness  (2)
  • Chickpea  (1)
  • 2020-2024
  • 1995-1999  (3)
  • 1
    Electronic Resource
    Electronic Resource
    Direct monitoring of Rhizobium sp. (Cicer) in nodules and soil using lac Z fusions (1996)
    Springer
    Biology and fertility of soils 21 (1996), S. 309-313 
    Details
    ISSN: 1432-0789
    Keywords: Nodulation ; Competitiveness ; Rhizobium sp. (Cicer) ; lacZ fusion
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract We constructed lacZ fusions in Rhizobium sp. (Cicer) by random Tn5-lacZ mutagenesis. The lacZ+ fusants formed blue colonies on a Rhizobial minimal medium containing 5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside (X-gal). Rhizobium sp. (Cicer) fusant HSL-2 was identified in nodules and soil in a mixed population on the basis of the lacZ+ phenotype. Nodule occupancy of inoculated Rhizobium sp. (Cicer) HSL-2 (lacZ+) was assessed by directly streaking the nodule sap on X-gal plates. This method revealed differences between rhizobia carrying identical antibiotic markers. The rhizobial population in soil was estimated by direct plate counts using a medium containing X-gal. Introduction of lacZ into the Rhizobium sp. thus provided a simple and direct method for identifying strains from nodules and soil.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00334908
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  • 2
    Electronic Resource
    Electronic Resource
    Direct monitoring of Rhizobium sp. (Cicer) in nodules and soil using lac Z fusions (1996)
    Springer
    Biology and fertility of soils 21 (1996), S. 309-313 
    Details
    ISSN: 1432-0789
    Keywords: Key words Nodulation ; Competitiveness ; Rhizobium sp. (Cicer) ; lacZ fusion
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract We constructed lacZ fusions in Rhizobium sp. (Cicer) by random Tn5-lacZ mutagenesis. The lacZ+ fusants formed blue colonies on a Rhizobial minimal medium containing 5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside (X-gal). Rhizobium sp. (Cicer) fusant HSL-2 was identified in nodules and soil in a mixed population on the basis of the lacZ+ phenotype. Nodule occupancy of inoculated Rhizobium sp. (Cicer) HSL-2 (lacZ+) was assessed by directly streaking the nodule sap on X-gal plates. This method revealed differences between rhizobia carrying identical antibiotic markers. The rhizobial population in soil was estimated by direct plate counts using a medium containing X-gal. Introduction of lacZ into the Rhizobium sp. thus provided a simple and direct method for identifying strains from nodules and soil.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00334908
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Abundance and polymorphism of di-, tri-and tetra-nucleotide tandem repeats in chickpea (Cicer arietinum L.) (1995)
    Springer
    Theoretical and applied genetics 90 (1995), S. 90-96 
    Details
    ISSN: 1432-2242
    Keywords: Chickpea ; Oligonucleotide fingerprinting ; Simple-sequence repeats ; Genetic diversity ; DNA polymorphism
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The abundance and polymorphism of 38 different simple-sequence repeat motifs was studied in four accessions of cultivated chickpea (Cicer arietinum L.) by in-gel hybridization of synthetic oligonucleotides to genomic DNA digested with 14 different restriction enzymes. Among 38 probes tested, 35 yielded detectable hybridization signals. The abundance and level of polymorphism of the target sequences varied considerably. The probes fell into three broad categories: (1) probes yielding distinct, polymorphic banding patterns; (2) probes yielding distinct, monomorphic banding patterns, and (3) probes yielding blurred patterns, or diffused bands superimposed on a high in lane background. No obvious correlation existed between abundance, fingerprint quality, and the sequence characteristics of a particular motif. Digestion with methyl-sensitive enzymes revealed that simple-sequence motifs are enriched in highly methylated genomic regions. The high level of intraspecific polymorphism detected by oligonucleotide fingerprinting suggests the suitability of simple-sequence repeat probes as molecular markers for genome mapping.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00221000
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