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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Plant cell reports 19 (2000), S. 634-637 
    ISSN: 1432-203X
    Keywords: Key words Lupinus luteus ; Yellow lupin ; Agrobacterium tumefaciens ; bar gene
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Transgenic yellow lupin (Lupinus luteus L.) plants have been generated by meristem co-cultivation with Agrobacterium tumefaciens. The binary plasmid pPZBNIa contains the bar gene under the control of a CaMV 35 S promoter. The transformation method involves inoculation of embryonic axis explants with A. tumefaciens, flooding the meristem with glufosinate, and initial culture on non-selective medium. Shoots were transferred to culture medium containing 20 mg/l glufosinate. Following subculture, shoots were grafted onto non-transgenic narrow-leafed lupin (L. angustifolius L.) seedling rootstocks, or rooted in vitro. The overall transformation efficiency, as determined at the T1 generation, was 0.05%–0.75%. The transgenic nature of plants grown to the T6 generation was confirmed by phosphinothricin acetyl transferase, PCR and Southern analyses.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Protoplasma 75 (1972), S. 381-395 
    ISSN: 1615-6102
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The occurrence and position of wall protuberances in giant cells induced in coleus roots by the root-knot nematodeMeloidogyne arenaria is described, and the structure and function of giant cells is compared with that of syncytia induced by cyst-nematodes. Extensive protuberance development occurs on walls of giant cells adjacent to xylem vessels. Protuberances are less well developed next to sieve elements, and almost absent next to parenchyma cells. On walls between giant cells they occur on both sides or only one side. The formation of protuberances indicates that giant cells are multinucleate transfer cells. The position of protuberances marks the wall area where solutes enter the cell. Solutes are obtained from xylem and phloem elements, and the position of protuberances at the junction between giant cells and vascular elements indicates an extensive flow of solutes along cell walls. The observations support the hypothesis that wall protuberances form as a result of selective solute flow across the plasmalemma. No cell wall dissolution was observed, although wall gaps may occur between giant cells as a result of breakage during rapid cell expansion.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Protoplasma 80 (1974), S. 401-405 
    ISSN: 1615-6102
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary In a study of membrane potential properties of giant cells induced in the roots ofImpatiens balsamina by a rootknot nematode, trains of action potential-like fluctuations were recorded. Giant cells are multinucleate transfer cells, and it is suggested that the occurrence of action potentials may be characteristic of transfer cells.
    Type of Medium: Electronic Resource
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  • 4
    Publication Date: 2021-06-02
    Description: We report the discovery of large amounts of previously undetected cold neutral atomic hydrogen (H i) around the core triplet galaxies in the nearby NGC 7232 galaxy group with MeerKAT. With a physical resolution of ∼1 kpc, we detect a complex web of low-surface-brightness H i emission down to a 4σ column density level of ∼1 × 1019 cm−2 (over 44  km s−1). The newly discovered H i streams extend over ∼20 arcmin corresponding to 140 kpc in projection. This is approximately three times the H i extent of the galaxy triplet (52 kpc). The H i debris has an H i mass of ∼6.6 × 109 M⊙, more than 50 per cent of the total H i mass of the triplet. Within the galaxy triplet, NGC 7233 and NGC 7232 have lost a significant amount of H i while NGC 7232B appears to have an excess of H i. The H i deficiency in NGC 7232 and NGC 7233 indicates that galaxy–galaxy interaction in the group concentrates on this galaxy pair while the other disc galaxies have visited them over time. In comparison to the AMIGA sample of isolated galaxies, we find that with regards to its total H i mass the NGC 7232/3 galaxy triplet is not H i-deficient. Despite the many interactions associated to the triplet galaxies, no H i seems to have been lost from the group (yet).
    Print ISSN: 0035-8711
    Electronic ISSN: 1365-2966
    Topics: Physics
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