ISSN:
1432-203X
Keywords:
Key words Lupinus luteus
;
Yellow lupin
;
Agrobacterium tumefaciens
;
bar gene
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Transgenic yellow lupin (Lupinus luteus L.) plants have been generated by meristem co-cultivation with Agrobacterium tumefaciens. The binary plasmid pPZBNIa contains the bar gene under the control of a CaMV 35 S promoter. The transformation method involves inoculation of embryonic axis explants with A. tumefaciens, flooding the meristem with glufosinate, and initial culture on non-selective medium. Shoots were transferred to culture medium containing 20 mg/l glufosinate. Following subculture, shoots were grafted onto non-transgenic narrow-leafed lupin (L. angustifolius L.) seedling rootstocks, or rooted in vitro. The overall transformation efficiency, as determined at the T1 generation, was 0.05%–0.75%. The transgenic nature of plants grown to the T6 generation was confirmed by phosphinothricin acetyl transferase, PCR and Southern analyses.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s002990050785
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