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  • Histochemistry  (8)
  • Springer  (8)
  • 2020-2024
  • 2010-2014
  • 1970-1974  (8)
  • 1945-1949
  • 1
    Electronic Resource
    Electronic Resource
    Ultrastructural and histochemical studies on the innervation of the heart of a teleost, pleuronectes platessa L. (1972)
    Springer
    Cell & tissue research 131 (1972), S. 519-528 
    Details
    ISSN: 1432-0878
    Keywords: Cardiac innervation ; Teleosts ; Acetylcholinesterase ; Fine structure ; Histochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The innervation of the heart of the plaice has been studied ultrastructurally and histochemically in order to describe the anatomy, and the nature of the neuro-transmitters involved in the regulation of the heart. A single cardiac branch of the vagus runs over the Duct of Cuvier and sinus venosus where it forms a plexus extending into the sinoatrial region. Many ganglion cells are scattered throughout this plexus upon which are seen cholinergic-type terminals at which AChE can be localised. It is therefore concluded that this is the parasympathetic cardiac ganglion. The innervation of the atrium is dense at the sinoatrial end but decreases towards the ventricle. The ventricle is probably aneural, possibly a consequence of the lack of a coronary blood supply to act as a pathway for an innervation of this chamber. Most of the intramural axon profiles contain large numbers of small agranular vesicles and are cholinergic, AChE being localised at their membranes. Other profiles contain small numbers of larger granular vesicles amongst the agranular vesicles but these are not adrenergic since these vesicles are not depleted by reserpine, do not load with 6-OHDA, and AChE can be localised at the membranes of the profiles. No adrenergic-type profiles were seen in any part of the heart, nor were any fluorescent, catecholamine-containing fibres observed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00306968
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  • 2
    Electronic Resource
    Electronic Resource
    Morphologische und funktionelle Befunde nach Milchsäuredauerinfusion in das Jejunum der Ratte (1972)
    Springer
    Cell & tissue research 132 (1972), S. 107-129 
    Details
    ISSN: 1432-0878
    Keywords: Jejunum (rat) ; Lactic acid perfusion ; Mucosal hypertrophy ; Histology ; Histochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung An weiblichen Wistarratten wurden die zellulären und strukturellen Veränderungen der Dünndarmschleimhaut nach chronischer MS-Belastung durch 12tägige Dauerinfusion ins Jejunum untersucht. Folgende Befunde wurden erhoben: 1. Die MS-Belastung führt in Abhängigkeit von der Konzentration zur Reduktion des Glukosetransports. 2. Die Funktionsstörung ist bedingt durch Enzymaktivitätsverlust. 3. Die Reduktion betrifft alle enzymhistochemisch untersuchten Aktivitäten; sie zeigt, daß alle Zellkompartimente des Epithels beeinträchtigt sind. 4. Qualitative Abweichungen des Reaktionsmusters treten nach MS-Belastung nur beim Saure-Phosphatase-Nachweis auf. Sie sind gekennzeichnet durch Schwund der Markierbarkeit lysosomaler Granula und Auftreten einer schwachen, diffusen cytoplasmatischen Aktivität. 5. Die Schleimhaut unterliegt einer von der MS-Konzentration abhängigen Transformation mit Verschiebung der Zottenhöhen und Kryptentiefen. Sie wird begleitet von einer gesteigerten mitotischen Aktivität als Folge erhöhter Zellexfoliation an der Zottenspitze. Es handelt sich bei dieser Veränderung somit um eine Schleimhauttransformation vom hyper-regeneratorischen Typ. Ihr Initialstadium ist eine Hypertrophie, ihr Endstadium die zottenlose Schleimhaut mit stark veränderten Krypten. Das Endstadium wird unter MS-Belastung nicht ausgebildet, da geringe Konzentrationssteigerungen zu lokaler Gewebsschädigung und Darmperforation führen. 6. Das MS-Modell ist wegen seiner geringen experimentellen Breite ungeeignet für die biochemische Nacharbeitung der bei der einheimischen Sprue beobachteten cytochemischen Epithelveränderungen.
    Notes: Summary Cellular alterations and mucosal changes in zonation of the small intestine have been studied after prolonged perfusion (12 days) with lactic acid in the rat. These investigations were carried out with cytochemical methods using marker enzymes to study in detail the different stages of mucosal transformation of the hyperregenerative type. Results. 1. Lactic acid produces a dose-dependent reduction in glucose absorption and this is due to alterations of the absorptive epithelium. 2. The normal zonation of the small intestinal mucosa is changed. The degree and pattern of the alterations is dose-dependent. Mucosal hypertrophy—i.e. increase of villus height, crypt length and mitotic activity—is the initial stage of this process. 3. Both, cellular alterations and mucosal changes in zonation are the expression of strong compensatory activities in response to predominantly intraluminal stress. The relationship between mucosal hypertrophy after intestinal resection and mucosal transformation of the hyperregenerative type as induced by lactic acid infusion is discussed. 4. In view of its very limited experimental range the lactic acid model cannot be recommended for the study of the epithelial changes in mucosal transformation at a biochemical level.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00310300
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  • 3
    Electronic Resource
    Electronic Resource
    Über den Peroxydasetransport im Darmepithel der Ratte (1972)
    Springer
    Cell & tissue research 134 (1972), S. 403-410 
    Details
    ISSN: 1432-0878
    Keywords: Small intestine (rat) ; Transport of peroxidase ; Epithelium ; Histochemistry ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Bei Ratten tritt 3 min nach intravenöser Injektion von Peroxydase elektronenmikroskopisch ein entsprechendes Reaktionsprodukt im Kapillarlumen der Lamina propria des Dünndarms und an der Basalmembrangrenze der Saumepithelzellen auf. 5 min nach der Injektion finden sich im basalen Abschnitt des Darmepithels pinozytotische Bläschen mit dem Peroxydase-Reaktionsprodukt. — 10–30 min nach der Injektion erreichen die Partikel die apikalen Teile der Zelle. Sie dringen in den interzellulären Spalten bis zu den Haftplatten vor, erreichen jedoch nie das Darmlumen. Im Dünndarm existiert vermutlich auch ein der Resorption entgegengesetzter Saftstrom, der durch Peroxydase markiert werden kann.
    Notes: Summary In rats, 3 minutes after intravenous injection of peroxidase the reaction product can be observed electronmicroscopically in the lumina of the capillaries of the small intestine as well as at the border of the basement membrane of the epithelial border cells. Pinocytotic vesicles containing peroxidase particles occur in the basal portion of the epithelium of the small intestine 5 minutes after injection. 10–30 minutes later, the peroxidase reaches the apical region of the cell. The particles infiltrate into the intercellular spaces as far as the tight junctions but never reach the intestinal lumen. In the small intestine there probably exists a flow of fluid in opposite direction to the resorption, which can be marked by peroxidase.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00307174
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  • 4
    Electronic Resource
    Electronic Resource
    Axoplasmic flow of adrenaline and monoamine oxidase in amphibian sympathetic nerves (1972)
    Springer
    Cell & tissue research 124 (1972), S. 44-56 
    Details
    ISSN: 1432-0878
    Keywords: Axonal transport ; Toad spinal nerves ; Ligation ; Sympathectomy ; Histochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The accumulation of both A and MAO proximal to a ligature on toad spinal nerves has been shown to occur at a slower rate than in mammals. As in mammals, there are two components of axonal transport in amphibian nerves, with the accumulation of A reaching a peak at between 4 and 7 days (cf. 2–4 days for NA in mammals), while MAO accumulation does not reach its maximum before 9 days (cf. 7 days in mammals). No accumulation occurs after sympathectomy, providing evidence for localization of MAO within amphibian sympathetic adrenergic nerves. Distal accumulation of MAO occurs in toad sympathetic nerves; this has not been reported to occur in mammalian nerves. Distal accumulation reaches a peak at 2–4 days, which suggests either a fast retrograde flow of MAO or that induction of MAO is occurring. These results are discussed in relation to differences between mammalian and amphibian sympathetic nerves and to the events occurring following ligation of these nerves.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00335453
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  • 5
    Electronic Resource
    Electronic Resource
    Axoplasmic flow of adrenaline and monoamine oxidase in amphibian sympathetic nerves (1972)
    Springer
    Cell & tissue research 124 (1972), S. 44-56 
    Details
    ISSN: 1432-0878
    Keywords: Axonal transport ; Toad spinal nerves ; Ligation ; Sympathectomy ; Histochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The accumulation of both A and MAO proximal to a ligature on toad spinal nerves has been shown to occur at a slower rate than in mammals. As in mammals, there are two components of axonal transport in amphibian nerves, with the accumulation of A reaching a peak at between 4 and 7 days (cf. 2–4 days for NA in mammals), while MAO accumulation does not reach its maximum before 9 days (cf. 7 days in mammals). No accumulation occurs after sympathectomy, providing evidence for localization of MAO within amphibian sympathetic adrenergic nerves. Distal accumulation of MAO occurs in toad sympathetic nerves; this has not been reported to occur in mammalian nerves. Distal accumulation reaches a peak at 2–4 days, which suggests either a fast retrograde flow of MAO or that induction of MAO is occurring. These results are discussed in relation to differences between mammalian and amphibian sympathetic nerves and to the events occurring following ligation of these nerves.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00981940
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  • 6
    Electronic Resource
    Electronic Resource
    Elektronenmikroskopische und histochemische Untersuchungen zur Funktion der Branchialdrüse (Parabranchialdrüse) der Cephalopoda (1973)
    Springer
    Cell & tissue research 139 (1973), S. 253-269 
    Details
    ISSN: 1432-0878
    Keywords: Branchial gland ; Cephalopoda ; Fine structure ; Hemocyanin ; Histochemistry ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Licht- und elektronenmikroskopische Untersuchungen an der Branchialdrüse verschiedener Cephalopoden (Sepia officinalis, Octopus vulgaris, Loligo vulgaris, Eledone moschata) zeigen, daß das Drüsengewebe des stark vaskularisierten Organs aus einem sekretorisch aktiven Zelltypus mit ausgeprägtem endoplasmatischen Retikulum besteht. In den Zisternen des endoplasmatischen Retikulums lassen sich granuläre und ringförmige Strukturen (Durchmesser: 45–65 Å und 170 Å) nachweisen, die in Größe und Gestalt mit den Hämocyanin-Einheiten in den Gefäßen und Lakunen übereinstimmen und durch randlichen Zerfall der Zellen in den Blutraum auszuwandern scheinen. Die histochemischen Untersuchungen ergeben eine positive Reaktion für Monoaminoxydase, aber vergleichsweise geringe Phosphatase- und Dehydrogenase-Aktivitäten. Diese Befunde sowie der histochemisch nachgewiesene hohe Kupfergehalt des Drüsengewebes sprechen für eine hämopoetische, d.h. hämocyaninbildende Funktion der Kiemendrüse.
    Notes: Summary Light and electron microscopic investigations on the branchial gland of different Cephalopoda (Sepia officinalis, Octopus vulgaris, Loligo vulgaris, Eledone moschata) show that the gland tissue, strongly vascularized, consists of an actively secreting cell type with a remarkable endoplasmatic reticulum. In the cisternae of the endoplasmatic reticulum granular and ring-shaped structures can be identified (diameter: 45–65 Å and 170 Å) which correspond in size and shape with the hemocyanin units in the vessels and lacunae and which seem to emigrate into the blood space caused by the peripheral decay of the cells. Histochemical investigations show a positive reaction for monoaminoxidase, but comparatively low phosphatase and dehydrogenase activities. These results, and also the high content of histochemically identified copper in the gland tissue, seem to indicate a hemopoetic, i.e. hemocyanin-forming function for the branchial gland.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00306525
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  • 7
    Electronic Resource
    Electronic Resource
    The distribution of monoamines in the tel-, di-and mesencephalon of Xenopus laevis tadpoles, with special reference to the hypothalamo-hypophysial system (1973)
    Springer
    Cell & tissue research 137 (1973), S. 521-540 
    Details
    ISSN: 1432-0878
    Keywords: Brain ; Hypothalamo-hypophysial system ; Xenopus laevis, tadpoles ; Monoamines ; Histochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary In tadpoles of Xenopus laevis the histochemical distribution of monoamines in the tel-, di- and mesencephalon was studied. Catecholamines are present in some mitral cells of the olfactory bulb, the neurons of the preoptic recess organ (PRO), the neurons of the paraventricular organ (PVO) and cells in the vicinity of the PVO, namely the so-called PVO-accompanying cells, the nucleus infundibularis dorsalis (NID) and in the nucleus reticularis mesencephali (NRM). The PRO, PVO, and NID are composed of liquor-contacting neurons. With the Falck-technique, in the PVO and NID two types of fluorescent cells were observed: green cells, containing a catecholamine and yellow-orange cells, presumably containing a tryptamine. Tryptaminergic neurons occur in the NRM and in the area of the raphe nucleus. Extensive tracts of fluorescent fibres originating in the hindbrain ascend to the area praeoptica and the septum, striatum and epistriatum of the telencephalon. Also, fibre connections could be traced from the PVO to the area praeoptica, to the thalamus and to the nucleus ventromedialis tuberis. The PVO and/or NID give rise to a catecholaminergic tract running towards the hypophysis. Fibres of this tract partly terminate in the developing median eminence and partly in the pars intermedia. Experimental data indicate that the aminergic fibres in the pars intermedia exert an inhibitory influence on MSH-release.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00307228
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  • 8
    Electronic Resource
    Electronic Resource
    Histological and histochemical studies on the rhesus monkey (Macaca mulatta) olfactory mucosa (1970)
    Springer
    Cell & tissue research 103 (1970), S. 291-319 
    Details
    ISSN: 1432-0878
    Keywords: Primates ; Sense organs ; Olfactory Mucosa ; Histochemistry ; Histology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Detailed histochemical studies on the localization of various oxidative and dephosphorylating enzymes and esterases have been made on the olfactory mucosa of the rhesus monkey. The upper part of dendritic process and terminal rods of receptor cells and the upper part of supporting cells show high oxidative enzyme activity. The soma and infranuclear parts of receptor and supporting cells have much lower oxidative enzyme activity. The basal cells and perineural epithelial cells have high hydrolytic enzyme activity, though they show low oxidative enzyme activity. Alkaline phosphatase is localized in the olfactory axons, basal cells and perineural epithelial cells. The receptor and supporting cells contain much less adenosine triphosphatase activity than the basal cells and perineural epithelial cells. The axons of receptor cells pass between the basal cells and come out from the basal cell layer forming the olfactory nerve fasciculi, most of which are surrounded completely by flat squamous cells starting from the base of the basal cell layer. The basal cells are histochemically and cytologically related to the perineural epithelial cells covering the olfactory nerve fasciculi. Basal cells probably cannot give rise to supporting cells and are not young forms of supporting cells. The degenerating receptor cells as well as the olfactory nerve fasciculi which are not completely covered by the flat squamous cells may make the locus minoris resistentiae in the olfactory mucosa. The olfactory axons are rich in most of enzymes we have tested. They seem to be metabolically very active and both cholinergic and adrenergic in nature. The mucosal secretion covering the olfactory epithelium contains oxidative and some hydrolytic enzymes. Bowman's glands show high activity for most enzymes except cholinesterases.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00335275
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