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  • 1
    Electronic Resource
    Electronic Resource
    Electroporation of cells (1990)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 79 (1990), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: By measuring uptake of the membrane impermeable dye. phenosafranine, it can be shown that the plasma membrane of intact cells within cell aggregates can be reversibly permeabilized by electroporation. However, the plant cell wall is a barrier to DNA uptake by intact cells, although under certain circumstances expression of DNA, electroporated into intact cells, can be demonstrated. The level of expression is about 20–50 times lower than that obtained by electroporation of protoplasts, and depends on cell wall properties and pretreatments of cell aggregates. In contrast, efficient transformation of whole cells of bacteria and yeasts can be achieved by electroporation. Factors which influence DNA transfer into whole plant cells and the possibility of stable transformation are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1990.tb05881.x
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  • 2
    Electronic Resource
    Electronic Resource
    Transgenic yellow lupin (Lupinus luteus) (2000)
    Springer
    Plant cell reports 19 (2000), S. 634-637 
    Details
    ISSN: 1432-203X
    Keywords: Key words Lupinus luteus ; Yellow lupin ; Agrobacterium tumefaciens ; bar gene
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Transgenic yellow lupin (Lupinus luteus L.) plants have been generated by meristem co-cultivation with Agrobacterium tumefaciens. The binary plasmid pPZBNIa contains the bar gene under the control of a CaMV 35 S promoter. The transformation method involves inoculation of embryonic axis explants with A. tumefaciens, flooding the meristem with glufosinate, and initial culture on non-selective medium. Shoots were transferred to culture medium containing 20 mg/l glufosinate. Following subculture, shoots were grafted onto non-transgenic narrow-leafed lupin (L. angustifolius L.) seedling rootstocks, or rooted in vitro. The overall transformation efficiency, as determined at the T1 generation, was 0.05%–0.75%. The transgenic nature of plants grown to the T6 generation was confirmed by phosphinothricin acetyl transferase, PCR and Southern analyses.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990050785
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  • 3
    Electronic Resource
    Electronic Resource
    Plant regeneration from protoplasts of Solanum species with potential agricultural value (S. hjertingii, S. polyadenium, S. capsicibaccatum) (1991)
    Springer
    Plant cell reports 9 (1991), S. 520-522 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Protoplasts have been isolated from three tuber-bearing Solanum species, S. hjertingii, S. polyadenium and S. capsicibaccatum, that are sexually incompatible with S. tuberosum, but possess potentially useful characters. For isolating protoplasts from leaves of in vitro shoot cultures of S. hjertingii and S. capsicibaccatum growth was improved by including silver thiosulfate in the medium. However, for S. polyadenium, leaves of pot-grown plants were the best source for protoplasts. Following protoplast division and culture, plants were regenerated from protoplasts of each of the species. The pattern of chromosome variation in regenerants was similar to that observed for other diploid and tetraploid Solanum species. The results indicate that it should be possible to introduce the potentially useful germplasm from these wild species into somatic hybrids with S. tuberosum by protoplast fusion.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00232110
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  • 4
    Electronic Resource
    Electronic Resource
    Species-specific sequences in the genus Solanum: identification, characterization, and application to study somatic hybrids of S. brevidens and S. tuberosum (1990)
    Springer
    Theoretical and applied genetics 80 (1990), S. 693-698 
    Details
    ISSN: 1432-2242
    Keywords: Potato ; Solanum brevidens ; Somatic hybrids ; Species-specific probes ; Squash blots
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary To aid in the identification and analysis of somatic hybrids between potato (Solanum tuberosum, dihaploid line PDH 40) and the non tuber-bearing wild species S. brevidens, a series of species-specific repetitive DNA sequences have been isolated. This was accomplished by making libraries of HaeIII-digested total DNA of S. tuberosum and S. brevidens, by cloning fragments into the SmaI site of plasmid pUC18 and transforming them into E. coli (JM83). The S. brevidens library consisted of 1,000 recombinant clones, and that of S. tuberosum, 700. Nitrocellulose filters with recombinant clones were hybridised to nick-translated total DNA of S. brevidens and also S. tuberosum, and, following autoradiography, clones that hybridised strongly to the DNA of only one of the species were chosen. Two highly repeated S. brevidens clones (pSB1, 400 bp and pSB7,210 bp), one highcopy-number s. tuberosum clone (pST10, 200 bp) and one low-copy-number sequence of S. tuberosum (pST3, 1.5 kbp) were selected for further analysis by Southern hybridisation to digested total DNA. Clone pSB7 gave a ladder pattern on hybridisation to EcoR1-digested total DNA of S. brevidens, with signals at multiples of 200 bp DNA. Using these probes it was possible to verify the hybridity of putative hybrids of dihaploid S. tuberosum and S.brevidens, and to confirm by Southern analysis and by slot blots the parental genome dosage of hexaploid hybrids (two s. brevidens: one S. tuberosum, and vice-versa). The S. tuberosum-specific probe, pSTIO, hybridised with DNA of three other tuber-bearing wild species (S. hjertingii, S. capsicibaccatum and S. berthaultii). A squash-blot procedure was developed using the probes that would allow early identification of somatic hybrid callus. There are a number of useful applications of such species-specific probes in the identification and analysis of somatic hybrids.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00224231
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  • 5
    Electronic Resource
    Electronic Resource
    Analysis of the mitochondrial DNA of the somatic hybrids of Solanum brevidens and S. tuberosum using non-radioactive digoxigenin-labelled DNA probes (1993)
    Springer
    Theoretical and applied genetics 85 (1993), S. 1017-1022 
    Details
    ISSN: 1432-2242
    Keywords: Potato ; S. brevidens ; Mitchondrial DNA ; Somatic hybrids ; Non-radioactive labelling
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Mitochondrial (mt) DNAs of somatic hybrids obtained by electrical and chemical fusion of mesophyll protoplasts of S. brevidens and a dihaploid line of S. tuberosum PDH 40 were analysed by Southern hybridization using the digoxigenin-labelled mtDNA sequences nad5 or orf25. In the Southern analysis of the hybrid mtDNA probed with nad5, most of the 19 hybrids analyzed had an RFLP pattern similar, but not identical, to one of the parents, S. tuberosum, PDH40. Nineteen percent of the hybrids had most of the S. brevidens fragments. Five of the hybrids had an identical RFLP pattern to either one of the parents while another two hybrids had novel RFLP patterns. Similar results were obtained by Southern analysis with orf25. These results clearly show that mtDNA rearrangements had occurred at a high frequency in the somatic hybrids. There were no differences in the frequencies of rearrangements observed between the hybrids regenerated from chemical and electrical fusions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00215042
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  • 6
    Electronic Resource
    Electronic Resource
    Production of asymmetric hybrids between Solanum tuberosum and irradiated S. brevidens (1993)
    Springer
    Theoretical and applied genetics 85 (1993), S. 729-734 
    Details
    ISSN: 1432-2242
    Keywords: Protoplast fusion ; Gamma irradiation ; Partial genome transfer ; Solanum tuberosum ; Solanum brevidens
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Asymmetric somatic hybrids were obtained by fusion of Solanum tuberosum (PDH40) protoplasts with 300- or 500-Gy irradiated protoplasts of S. brevidens. These radiation doses were sufficient to prevent the growth of the S. brevidens protoplasts. Putative hybrids were selected on the basis of phenotype from regenerated shoots and identified with a S. brevidens-specific probe. From these, 31 asymmetric hybrids were confirmed by morphological characteristics, isoenzyme patterns and RFLP analysis. The morphology of the asymmetric hybrids was intermediate between that of S. tuberosum and symmetric hybrids of both species (obtained without irradiation treatment). Chromosome counts from 17 asymmetric hybrids showed that the chromosome number of the hybrids ranged from 31 to 64. The asymmetric hybrids probably had one or two genome complements (i.e. either 24 or 48 chromosomes) from S. tuberosum and 7–22 chromosomes from S. brevidens. There was no clear correlation between the radiation dose and the degree of elimination of the S. brevidens genome.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00225012
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  • 7
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    When is stacking confusing? The impact of confusion on stacking in deep H I galaxy surveys (2015)
    Oxford University Press
    Details
    Publication Date: 2015-11-19
    Description: We present an analytic model to predict the H i mass contributed by confused sources to a stacked spectrum in a generic H i survey. Based on the ALFALFA (Arecibo Legacy Fast ALFA) correlation function, this model is in agreement with the estimates of confusion present in stacked Parkes telescope data, and was used to predict how confusion will limit stacking in the deepest Square Kilometre Array precursor H i surveys. Stacking with LADUMA (Looking At the Distant Universe with MeerKAT) and DINGO UDEEP (Deep Investigation of Neutral Gas Origins – Ultra Deep) data will only be mildly impacted by confusion if their target synthesized beam size of 10 arcsec can be achieved. Any beam size significantly above this will result in stacks that contain a mass in confused sources that is comparable to (or greater than) that which is detectable via stacking, at all redshifts. CHILES (COSMOS H i Large Extragalactic Survey) 5 arcsec resolution is more than adequate to prevent confusion influencing stacking of its data, throughout its bandpass range. FAST (Five hundred metre Aperture Spherical Telescope) will be the most impeded by confusion, with H i surveys likely becoming heavily confused much beyond z  = 0.1. The largest uncertainties in our model are the redshift evolution of the H i density of the Universe and the H i correlation function. However, we argue that the two idealized cases we adopt should bracket the true evolution, and the qualitative conclusions are unchanged regardless of the model choice. The profile shape of the signal due to confusion (in the absence of any detection) was also modelled, revealing that it can take the form of a double Gaussian with a narrow and wide component.
    Print ISSN: 0035-8711
    Electronic ISSN: 1365-2966
    Topics: Physics
    Published by Oxford University Press
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  • 8
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    Environmental dependence of the H I mass function in the ALFALFA 70% catalogue (2016)
    Oxford University Press
    Details
    Publication Date: 2016-03-08
    Description: We search for environmental dependence of the H  i mass function in the Arecibo Legacy Fast ALFA survey (ALFALFA) 70 per cent catalogue. The catalogue is split into quartiles of environment density based on the projected neighbour density of neighbours found in both Sloan Digital Sky Survey (SDSS) and 2MASS Redshift Survey (2MRS) volume-limited reference catalogues. We find the Schechter function ‘knee’ mass to be dependent on environment, with the value of log ( M * /M ) shifting from 9.81 ± 0.02 to 10.00 ± 0.03 between the lowest and highest density quartiles. However, this dependence was only observed when defining environment based on the SDSS reference catalogue, not 2MRS. We interpret these results as meaning that the local environment is the dominant cause of the shift in M * , and that the larger scales that 2MRS probes (compared to SDSS) are almost irrelevant. In addition, we also use a fixed aperture method to probe environment, and find tentative evidence that H  i -deficiency depresses the value of M * in the highest density regions. We find no significant dependence of the low-mass slope on environment in any test, using either method. Tensions between these results and those from the literature, are discussed and alternative explanations are explored.
    Print ISSN: 0035-8711
    Electronic ISSN: 1365-2966
    Topics: Physics
    Published by Oxford University Press
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  • 9
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    Spectroscopic confusion: its impact on current and future extragalactic H I surveys (2015)
    Oxford University Press
    Details
    Publication Date: 2015-03-28
    Description: We present a comprehensive model to predict the rate of spectroscopic confusion in H  i surveys, and demonstrate good agreement with the observable confusion in existing surveys. Generically the action of confusion on the H  i mass function was found to be a suppression of the number count of sources below the ‘knee’, and an enhancement above it. This results in a bias, whereby the ‘knee’ mass is increased and the faint end slope is steepened. For ALFALFA and HIPASS, we find that the maximum impact this bias can have on the Schechter fit parameters is similar in magnitude to the published random errors. On the other hand, the impact of confusion on the H  i mass functions of upcoming medium depth interferometric surveys, will be below the level of the random errors. In addition, we find that previous estimates of the number of detections for upcoming surveys with Square Kilometre Array-precursor telescopes may have been too optimistic, as the framework implemented here results in number counts between 60 and 75 per cent of those previously predicted, while accurately reproducing the counts of existing surveys. Finally, we argue that any future single dish, wide area surveys of H  i galaxies would be best suited to focus on deep observations of the local Universe ( z  〈 0.05), as confusion may prevent them from being competitive with interferometric surveys at higher redshift, while their lower angular resolution allows their completeness to be more easily calibrated for nearby extended sources.
    Print ISSN: 0035-8711
    Electronic ISSN: 1365-2966
    Topics: Physics
    Published by Oxford University Press
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  • 10
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    Environmental dependence of the H I mass function in the ALFALFA 70% catalogue (2016)
    Oxford University Press
    Details
    Publication Date: 2016-03-08
    Description: We search for environmental dependence of the H  i mass function in the Arecibo Legacy Fast ALFA survey (ALFALFA) 70 per cent catalogue. The catalogue is split into quartiles of environment density based on the projected neighbour density of neighbours found in both Sloan Digital Sky Survey (SDSS) and 2MASS Redshift Survey (2MRS) volume-limited reference catalogues. We find the Schechter function ‘knee’ mass to be dependent on environment, with the value of log ( M * /M ) shifting from 9.81 ± 0.02 to 10.00 ± 0.03 between the lowest and highest density quartiles. However, this dependence was only observed when defining environment based on the SDSS reference catalogue, not 2MRS. We interpret these results as meaning that the local environment is the dominant cause of the shift in M * , and that the larger scales that 2MRS probes (compared to SDSS) are almost irrelevant. In addition, we also use a fixed aperture method to probe environment, and find tentative evidence that H  i -deficiency depresses the value of M * in the highest density regions. We find no significant dependence of the low-mass slope on environment in any test, using either method. Tensions between these results and those from the literature, are discussed and alternative explanations are explored.
    Print ISSN: 0035-8711
    Electronic ISSN: 1365-2966
    Topics: Physics
    Published by Oxford University Press
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