ISSN:
0021-9541
Keywords:
Life and Medical Sciences
;
Cell & Developmental Biology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Biology
,
Medicine
Notes:
Giant squid axons were microinjected with serine, valine and leucine-C14 under controlled electrophysiological conditions. These amino acids are incorporated into TCA insoluble fraction in the isolated axon.This incorporation is higher in the stimulated axons as compared to non-stimulated ones. By processing separately the axoplasm and axon sheath, it was found that the last one is responsible almost entirely for the observed incorporation.Through differential centrifugation of homogenates of microinjected axons was shown that the highest incorporation occurred in the 1500 × g sediment, which probably corresponds to membranes.The incorporation of amino acids in stimulated axons, is strongly inhibited by chloramphenicol and actinomycin D.
Additional Material:
1 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/jcp.1040700110
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