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  • 1
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Singapore journal of tropical geography 13 (1993), S. 0 
    ISSN: 1467-9493
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Geography
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Water and environment journal 5 (1991), S. 0 
    ISSN: 1747-6593
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: Wessex Water has developed a new 35 Ml/d water supply source, using lakes formed from worked-out gravel pits, to meet increasing demands for water and to provide security for existing supplies.Having identified the need for the scheme, an engineering feasibility study was carried out in conjunction with a full environmental assessment. This led to the successful promotion of the Stage I scheme in 1986. Design and construction took place immediately after, and the scheme was officially opened in 1989.There are opportunities to expand the scheme as more lakes are formed by gravel working, and proposals for this are well advanced. This paper describes all the key elements in the promotion and development of the scheme.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Water and environment journal 5 (1991), S. 0 
    ISSN: 1747-6593
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: This paper provides a method of identifying land contaminated with chlorinated hydrocarbon solvents by sampling and analysing soil gas. Relationships are presented which enable levels of soil gas contamination to be related to levels of soil and groundwater pollution. Two case studies are presented. Soil gas surveying of an industrial site with severe groundwater pollution demonstrated that standards for contaminated land are insufficient to protect groundwater. Solvent levels in groundwater were one to two orders of magnitude higher than UK permitted concentrations in drinking water, whereas soil contamination values did not reach polluted levels. Surveying of a site occupied by a disused fire station showed significant differences in patterns of soil gas and groundwater pollution. This is explained by direct pollution of groundwater though the well at the site and/or possible flow of polluted groundwater to the site from adjacent areas.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Molecular microbiology 13 (1994), S. 0 
    ISSN: 1365-2958
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: The region immediately downstream from the miaA tRNA modification gene at 94.8 min contains the hfq gene and the hflA region, which are important in the bacteriophage Qβ and lambda life cycles. The roles of these genes in bacteria remain largely unknown. We report here the characterization of two chromosomal hfq insertion mutations. An omega (ω) cassette insertion near the end of hfq resulted in phenotypes only slightly different from the parent, although transcript mapping demonstrated that the insertion was completely polar on hfq expression. In contrast, an equally polar omega cassette insertion near the beginning of hfq caused pronounced pleiotropic phenotypes, including decreased growth rates and yields, decreased negative supercoiling of piasmids in stationary phase, increased cell size, osmosensitivity, increased oxidation of carbon sources, increased sensitivity to ultraviolet light, and suppression of bgl activation by hns mutations, hfq::ω mutant phenotypes were distinct from those caused by omega insertions early in the miaA tRNA modification gene. On the other hand, both hfq insertions interfered with lambda phage plaque formation, probably by means of polarity at the hflA region. Together, these results show that hfq function plays a fundamental role in Escherichia coli physiology and that hfq and the hflA region are in the amiB-mutL-miaA-hfq-hflX superoperon.
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1365-2958
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: We report a molecular genetic analysis of the region Immediately upstream from the Escherichia coli mutL DNA repair gene at 94.8 min. An open reading frame ending 9bp upstream from the start of mutL corresponds to a 48kDa polypeptide detected previously in minicells. The predicted amino acid sequence of this 48kDa polypeptide shows homology to the major N-acetylmuramoyl-L-alanine amidase autolysin of Bacillus subtilis, a known amidase of Bacillus licheniformis, and the product of a Salmonella typhimurium gene that maps near SO min. Insertions in this upstream gene, which we named AmiB, or in mutL did not affect cell shape or viability; however, overexpression of the AmiB potypeptide caused ceil lysis, hypersensitivity to osmotic shock and treatment with water, and temporary autolysis by low levels of antibiotics, which are all consistent with AmiB acting as a cell-wall hydrolase. Analysis of chromosomal transcription demonstrated that amiB forms a complex operon with mutL and two additional upstream genes. mutL transcripts also originated from an internal promoter, designated PmutL, located in amiB 312bp upstream from the translational start of mutL. Together, these results suggest that E. coli contains a second amidase possibly involved in cell-wall hydrolysis, septation, or recycling, and that transcription of this amidase is directly linked to a gene central for DNA repair.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Plant pathology 43 (1994), S. 0 
    ISSN: 1365-3059
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: More than 20000 wheat plants, derived from callus culture of immature wheat embryos were assessed for reaction to the take-all fungus (Gaeumannomyces graminis var. tritici) using a test-tube assay. Lines with less root and shoot blackening and more remaining green tissue than parental lines were identified in the SC3 generation. However these lines had more root and shoot blackening and less remaining green tissue in subsequent generations. The changes in reaction to the fungus may therefore have been epigenetic. The potential of this tissue culture derived material for breeding take-all resistant wheats is discussed.
    Type of Medium: Electronic Resource
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