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  • 04.03. Geodesy  (1)
  • 6-phosphofructo-2-kinase  (1)
  • 2020-2023  (1)
  • 2015-2019
  • 1995-1999  (1)
  • 1965-1969
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  • 2020-2023  (1)
  • 2015-2019
  • 1995-1999  (1)
  • 1965-1969
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  • 1
    Digitale Medien
    Digitale Medien
    Springer
    Molecular and cellular biochemistry 156 (1996), S. 125-130 
    ISSN: 1573-4919
    Schlagwort(e): fructose 2,6-bisphosphate ; 6-phosphofructo-2-kinase ; K562 cell differentiation ; MEG-01 cell differentiation
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie , Medizin
    Notizen: Abstract Fructose 2,6-bisphosphate (Fru-2,6-P2) represents the most powerful activator of 6-phosphofructo-l-kinase, rate-limiting enzyme of glycolysis. Fru-2,6-P2 content is tightly regulated and appears to be under the control of different hormones and growth factors, acting either through covalent modification of isoenzymatic forms of 6-phosphofructo-2-kinase/fructose 2,6-bisphosphatase (PFK-2/FBPase-2), the bifunctional enzyme responsible for the synthesis and the degradation of the compound, or through changes in transcription rate and/or in the expression of different isoforms of the enzyme. In the present study the metabolism of Fru-2,6-P2 was investigated during the differentiation toward megakaryocytes induced by phorbol 12-myristate 13-acetate (PMA) treatment of human leukemia K562 and MEG-O1 cell lines. Fru-2,6-P2 content as well as PFK-2 activity were increased in a dose-dependent manner after 4 days of incubation with PMA. MEG-01 cells resulted more sensitive to the effect of the inducer, anyway in both cell types cytostatic concentrations of the phorbol ester were able to affect Fru-2,6-P2 metabolism. The effect of PMA was maximal at 4 days of incubation in both examined cell lines. Interestingly, the effect induced by the phorbol ester at 4 days was still appreciable subculturing K562 and MEG-01 cells for 3 days in the absence of the inducer and was associated with relevant changes in the molecular properties of PFK-2: namely increased Vmax and Km. This latter finding suggests that the rise in Fru-2,6-P2 content during the differentiation process toward megakaryocytes might result from the expression of a novel PFK-2 isoform.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 2
    Publikationsdatum: 2022-12-01
    Beschreibung: The Global Navigation Satellite System (GNSS) represents a primary data source in Solid Earth Sciences. In order to investigate the Earth’s crustal deformation, time series of the estimated daily positions of the stations are routinely analyzed at the Istituto Nazionale di Geofisica e Vulcanologia (INGV) to investigate the deformation of the Earth’s surface caused by tectonic and non-tectonic processes. The GNSS observations of the stations are processed using the three main scientific software: GAMIT/GLOBK, BERNESE, and GIPSY OASIS II. The accuracy and the strength of geodetic solutions often depend on the geometry and spatial density of the network, and the availability and quality of GNSS data. In many circumstances, GNSS networks are deployed for topographic purposes by private or public institutions, and a significant number of GNSS stations in large regions acquire continuous observations. It may happen that such networks do not collect and distribute data according to IGS standards, so it could be difficult to analyze this data using automated data-processing tools. For that reason, this data is often ignored or partially used by the scientific community, despite their potential usefulness in geodynamic studies. We have attempted troubleshooting this problem by establishing a centralized storage facility in order to collect all available GNSS data and standardize both formats and metadata information. Here we describe the processes and functions that manage this unified repository, called MGA (Mediterranean GNSS Archive), which regularly collects GNSS RINEX files from alarge number of CORS (Continuously Operating Reference Station) located across a wide region of mainly the European and African plates. RINEX observation data and metadata information are provided to the analysts through an FTP server and dedicated web-services. The complete data set is stored in a PostgreSQL database in order to easily retrieve pieces of information and efficiently manage the archive content. The system implements many high-level services that include scripts to download files from remote archives and to detect new available data, web applications such as API (Application Program Interface) to interact with the system, and background services that interact with the database. During the development of this product, particular attention was paid to what has already been achieved by EPOS TCS WP10, whose objective was: "[...] to develop an open source platform with programmatic and web interfaces to store and disseminate raw data and metadata from GNSS stations operating in Europe''. Many ideas and tools presented here were inspired by that project.
    Beschreibung: Published
    Beschreibung: 1-18
    Beschreibung: 2T. Deformazione crostale attiva
    Beschreibung: 1IT. Reti di monitoraggio e sorveglianza
    Beschreibung: 4IT. Banche dati
    Beschreibung: N/A or not JCR
    Schlagwort(e): RINEX ; GNSS ARCHIVE ; GNSS MEDITERRANEAN AREA ; 04.03. Geodesy
    Repository-Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
    Materialart: article
    Standort Signatur Erwartet Verfügbarkeit
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