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  • Articles  (1,019)
  • 2020-2024  (69)
  • 2020-2023  (24)
  • 2005-2009  (926)
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  • 11
    Electronic Resource
    Electronic Resource
    The HOG MAP kinase pathway is required for the induction of methylglyoxal-responsive genes and determines methylglyoxal resistance in Saccharomyces cerevisiae (2005)
    Oxford, UK : Blackwell Science Ltd
    Molecular microbiology 56 (2005), S. 0 
    Details
    ISSN: 1365-2958
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: A sudden overaccumulation of methylglyoxal (MG) induces, in Saccharomyces cerevisiae, the expression of MG-protective genes, including GPD1, GLO1 and GRE3. The response is partially dependent on the transcriptional factors Msn2p/Msn4p, but unrelated with the general stress response mechanism. Here, we show that the high-osmolarity glycerol (HOG)-pathway controls the genetic response to MG and determines the yeast growth capacity upon MG exposure. Strains lacking the MAPK Hog1p, the upstream component Ssk1p or the HOG-dependent nuclear factor Msn1p, showed a reduction in the mRNA accumulation of MG-responsive genes after MG addition. Moreover, hyperactivation of Hog1p by deletion of protein phosphatase PTP2 enhanced the response, while blocking the pathway by deletion of the MAPKK PBS2 had a negative effect. In addition, the activity of Hog1p affected the basal level of GPD1 mRNA under non-inducing conditions. These effects had a great influence on MG resistance, as hog1Δ and other HOG-pathway mutants with impaired MG-specific expression displayed MG sensitivity, whereas those with enhanced expression exhibited MG resistance as compared with the wild-type. However, MG does not trigger the overphosphorylation of Hog1p or its nuclear import in the parental strain. Moreover, dual phosphorylation of Hog1p appears to be dispensable in the triggering of the transcriptional response, although a phosphorylable form of Hog1p is fundamental for the transcriptional activity. Overall, our results suggest that the basal activity of the HOG-pathway serves to amplify the expression of MG-responsive genes under non-inducing and inducing conditions, ensuring cell protection against this toxic glycolytic by-product.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2958.2005.04533.x
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  • 12
    Electronic Resource
    Electronic Resource
    A Novel Noninvasive Ultrasonic Method to Assess Avocado Ripening (2005)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 70 (2005), S. 0 
    Details
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: : A novel method to assess the ripening state of Persea Americana Mill avocados was developed. The method was validated and its results compared with standard ones such as the penetrometer method. Also, comparison were made between the proposed method and the ultrasonic system reported by other authors. The correlation between the proposed method and both the standard and former ultrasonic method is quite good. The measurement system overcomes the problem of alignment between the emitter and receiver found in the resent execution development for the same purposes. Extension of proposed systems could allow the measurement with a single ultrasonic source and possibility the development of an online evaluation system for all the fruits.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2621.2005.tb07134.x
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  • 13
    Electronic Resource
    Electronic Resource
    Heat shock treatments delay the increase in wound-induced phenylalanine ammonia-lyase activity by altering its expression, not its induction in Romaine lettuce (Lactuca sativa) tissue (2005)
    Oxford, UK; Malden, USA : Munksgaard International Publishers
    Physiologia plantarum 123 (2005), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Wounding lettuce (Lactuca sativa L., var. Longifolia) leaves induced an eight-fold increase in the activity of phenylalanine ammonia-lyase (PAL; EC 4.3.1.5), and the subsequent accumulation of phenolic compounds and tissue browning at 10°C. PAL is a key enzyme in the synthesis of phenolic compounds. A PAL cDNA was previously isolated by reverse-transcription PCR using total RNA from wounded lettuce leaves. RNA gel blots showed that maximum accumulation of both PAL mRNA and PAL enzyme activity occurred 24 h after wounding. A 2-min heat shock at 45°C administered within 5 min of wounding delayed the wound-induced increase in PAL activity, but did not delay the increase in wound-induced PAL mRNA. Changes in the content of PAL protein were also followed by immunoblot using anti-PAL antibody raised against the bacterially expressed protein from the cDNA. Immunoblots showed that the level of PAL protein in wounded lettuce tissue was significantly reduced by the heat shock treatment. These data suggest that heat shock reduces the rise in wound-induced PAL enzyme activity by reducing the translation of wound-induced PAL mRNA, or by increasing the turnover of the induced PAL protein.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.2005.00446.x
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  • 14
    Electronic Resource
    Electronic Resource
    Basalt and tonalite from the Amami Plateau, northern West Philippine Basin: New Early Cretaceous ages and geochemical results, and their petrologic and tectonic implications (2005)
    Melbourne, Australia : Blackwell Science Pty
    The @island arc 14 (2005), S. 0 
    Details
    ISSN: 1440-1738
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Geosciences
    Notes: Abstract  Basalts and tonalites dredged from the Amami Plateau in the northern West Philippine Basin have the geochemical characteristics of intraoceanic island arc rocks: low 87Sr/86Sr (0.70297–0.70310), intermediate 143Nd/144Nd (0.51288–0.51292), moderate light rare earth element (LREE) enrichment (La/Yb = 4.1–6.6) and high La/Nb (1.4–4.3). The incremental heating of hornblende from tonalites yielded well-defined plateaus and 40Ar/39Ar isochron ages of 115.8 ± 0.5 Ma and 117.0 ± 1.1 Ma, while plagioclase yielded disturbed Ar release patterns, with ages ranging from 70 to 112 Ma. Taken together, these results show that the Amami Plateau was formed by subduction-related magmatism in the Early Cretaceous period, earlier than indicated by prior K/Ar results. The results support tectonic models in which the West Philippine Basin was opened within a complex of Jurassic–Paleocene island arc terranes, which are now scattered in the northern West Philippine Basin, the Philippine Islands and Halmahera. The Amami Plateau tonalites and basalts have higher Sr/Y and lower Y and 87Sr/86Sr compared with younger tonalitic rocks from the northern Kyushu–Palau Ridge and the Tanzawa complex, which were formed by the subduction of the Pacific Plate beneath the Philippine Sea Plate. Based on the geochemical characteristics of the basalts, the Early Cretaceous subduction zone that formed the Amami Plateau may have been the site of slab melting, which suggests that a younger and hotter plate was being subducted at that time. However, the Amami tonalites were probably formed from basaltic magma by fractional crystallization or by partial melting of basaltic arc crust, rather than by melting of the subducted slab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1440-1738.2005.00474.x
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  • 15
    Electronic Resource
    Electronic Resource
    Production of cattle lacking prion protein (2007)
    [s.l.] : Nature Publishing Group
    Nature biotechnology 25 (2007), S. 132-138 
    Details
    ISSN: 1546-1696
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: [Auszug] Prion diseases are caused by propagation of misfolded forms of the normal cellular prion protein PrPC, such as PrPBSE in bovine spongiform encephalopathy (BSE) in cattle and PrPCJD in Creutzfeldt-Jakob disease (CJD) in humans. Disruption of PrPC expression in mice, a species that does not naturally ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/nbt1271
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  • 16
    Electronic Resource
    Electronic Resource
    The nuclear receptor LXR is a glucose sensor (2007)
    [s.l.] : Nature Publishing Group
    Nature 445 (2007), S. 219-223 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] The liver has a central role in glucose homeostasis, as it has the distinctive ability to produce and consume glucose. On feeding, glucose influx triggers gene expression changes in hepatocytes to suppress endogenous glucose production and convert excess glucose into glycogen or fatty acids ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/nature05449
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  • 17
    Electronic Resource
    Electronic Resource
    Insulin modulates gluconeogenesis by inhibition of the coactivator TORC2 (2007)
    [s.l.] : Nature Publishing Group
    Nature 449 (2007), S. 366-369 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] During feeding, increases in circulating pancreatic insulin inhibit hepatic glucose output through the activation of the Ser/Thr kinase AKT and subsequent phosphorylation of the forkhead transcription factor FOXO1 (refs 1–3). Under fasting conditions, FOXO1 increases ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/nature06128
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  • 18
    Electronic Resource
    Electronic Resource
    Deposition of Strontium-Doped Lanthanum Manganite Suspensions by Wet Powder Spraying (2008)
    s.l. ; Stafa-Zurich, Switzerland
    Materials science forum Vol. 591-593 (Aug. 2008), p. 459-464 
    Details
    ISSN: 1662-9752
    Source: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: The compound strontium-doped manganite lanthanum (La0,85Sr0,15MnO3 - LSM),deposited in the form of thin films in yttria-stabilized zirconia substrate (Y2O3/ZrO2 - YSZ), is ofbasic importance as cathodic material of the solid oxide fuel cells (SOFC). In this work, the LSMwas synthesized by the citrate technique and characterized by X-ray fluorescence spectroscopy(XRF), phase analysis light scattering granulometry (PALS), X-ray diffractometry (XRD) andscanning electron microscopy (SEM). In the wet powder spraying, was used an airbrush and theLSM sample deposited to the YSZ substrate was sintered and characterized by SEM. Theconclusions had allowed to establish preliminary conditions for preparation of LSM suspensions bywet powder spraying
    Type of Medium: Electronic Resource
    URL: http://www.tib-hannover.de/fulltexts/2011/0528/02/19/transtech_doi~10.4028%252Fwww.scientific.net%252FMSF.591-593.459.pdf
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  • 19
    Electronic Resource
    Electronic Resource
    Monitoring of Hydrocarbon Gaseous Compounds formed During Bioleaching of Copper Sulphide Minerals with Mesophilic Microorganisms (2007)
    s.l. ; Stafa-Zurich, Switzerland
    Advanced materials research Vol. 20-21 (July 2007), p. 139-142 
    Details
    ISSN: 1662-8985
    Source: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: An experimental campaign was conducted for monitoring the formation of hydrocarbongaseous compounds during bioleaching of copper sulphide ores with mesophilic microorganisms.Three different mineral samples were used: a pyrite concentrate, a chalcopyrite concentrate and acopper sulphide ore rock from El Teniente mine containing 1 % copper. Mineral samples werebioleached in 250 ml shake flasks containing 100 ml of basal medium inoculated either with a purestrain of Acidithiobacillus ferrooxidans or a natural bacteria consortium obtained from the acidleaching of El Teniente ore in columns. Each sealed system shake flask was fed with a flow ofsynthetic air and the exit stream was passed through a column containing an adsorbent material andnext through a water trap as to avoid back air contamination from the environment. Compoundspresent in the adsorbent material after 90 days of bioleaching were analysed using a gaschromatography mass spectrometry technique with a procedure that detects 162 differenthydrocarbon gaseous compounds (SGH). Results showed that the bacterial communities in coppersulphide bioleaching results in the formation of some hydrocarbon gaseous compounds, mainlyvarious types of methyl benzene and branched alkanes. The formation of these organic compounds islikely to be related to the presence of heterothrofic microorganisms present in the natural populations
    Type of Medium: Electronic Resource
    URL: http://www.tib-hannover.de/fulltexts/2011/0528/01/39/transtech_doi~10.4028%252Fwww.scientific.net%252FAMR.20-21.139.pdf
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  • 20
    Electronic Resource
    Electronic Resource
    Cellular Alignment Induction during Early 〈i〉In Vitro〈/i〉 Culture Stages UsingMicropatterned Glass Coatings Produced by Sol-Gel Process (2008)
    s.l. ; Stafa-Zurich, Switzerland
    Key engineering materials Vol. 396-398 (Oct. 2008), p. 303-306 
    Details
    ISSN: 1013-9826
    Source: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Cell behaviour such as adhesion, morphology, proliferation and functional activity are highly influenced by surface properties including hydrophobicity, roughness, texture and morphology. These surface properties may be controlled using a mixture of additive coating techniques to produce glass coatings by sol-gel process and soft lithography on dental ceramics. The purpose of this work was to compare cell adhesion and early orientation of Human Bone Marrow (HBM) cells cultured on micro-patterned (micro-PGC) and on flat glass coatings (FGC) produced by sol-gel processing. Spin coating was used to apply SiO2 flat coatings on glass substrates as model surfaces. Photolithography was applied to produce master patterns with microscale dimensions. A moulding technique was used to print micropatterned SiO2 glass coatings produced by a sol-gel process. The coatings were then sintered, sterilized and cultured with HBM cells derived from primary cultures, using a standardized protocol, for 1 and 7 days. Cell morphology and orientation were observed using scanning electron microscopy (SEM) and confocal laser scanning microscopy (CLSM). Flat and MPGC with line shaped features were produced. Cells presented a typical osteoblastic morphology on flat surfaces while slimmer, preferentially oriented and more elongated morphologies could be seen on line micro-patterned surfaces. HBM cells cultured on flat glass coatings showed increased tendency to spread and to assume more randomized proliferation when compared to the cells on the micro-patterned glass coatings. Micro-patterned glass coatings showed higher orientation control and smaller delay in the rate of proliferation, in early stages of in vitro culture as compared to flat coatings. These preliminary studies revealed that Micro-PGC induce significant morphological changes and controlled orientation of HBM cells during early stages of cell proliferation
    Type of Medium: Electronic Resource
    URL: http://www.tib-hannover.de/fulltexts/2011/0528/01/58/transtech_doi~10.4028%252Fwww.scientific.net%252FKEM.396-398.303.pdf
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