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  • 2020-2022  (14)
  • 1
    Publication Date: 2021-05-19
    Description: Chemical-ecological studies of sponges show that the secondary metabolites are only defense system. Nowadays natural products with biological activities are used as drugs for treatment of human diseases. One of the most important biological activities of sponges is cytotoxic activity. This study investigated the cytotoxic activities of α-Santonin compound which extracted from Dysidea avara. In this study, dried powder of sea sponge was extracted by acetone solvent. Then, in order to isolate the α-Santonin compound, the extract was washed by silica gel column chromatography with N-hexane and ethyl acetate solvents. The IC50 value of this compound was measured by XTT assay on cancer cell lines, Jurkat/E6-1, KB/C152 and healthy Hek293 cells. The α-Santonin compound with the chemical formula C15H18O3, which belongs to the Sesquiterpene-lactone group, was identified by gas chromatography, with a purity of 91% in fraction No. 24. The IC50 value of the α-Santonin extracted from sea sponge compared to the oral epithelial cancer cell line and lymphocyte cell line was 88.90 μg/ml and 38.09 μg/ml respectively. Our study demonstrated that α-Santonin extracted from D. avara has an extremely potent cytotoxic effect that could be used as an anticancer agent.
    Description: Published
    Keywords: Sponge ; LC50 ; Santonin ; Hengam Island
    Repository Name: AquaDocs
    Type: Journal Contribution , Refereed
    Format: pp.39-47
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  • 2
    Publication Date: 2021-05-19
    Description: Marine environment is the main source of natural bioactive products whose chemical-structural properties are not similar with other natural products of plants and other inland organisms. The present study was carried out to evaluate antibiotic properties of diethyl ether and methanol extracts of two marine species including starfish and carpet anemone. The samples of starfish and carpet anemone were collected from the Lark Island coasts. Extraction was done using diethyl ether and methanol solvents and antibiotic properties analysis on Escherichia coli and Bacillus subtilis were evaluated using agar diffusion method. Then tube dilution to determine the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). According to the results, diethyl ether extract from carpet anemone had no inhibitory effect on E. coli bacteria. While, starfish methanol extract (40 mg/ml) showed a growth inhibitory effect on E. coli bacteria. The starfish methanol extract (30 and 50 mg/ml) had a growth inhibitory effect on B. subtilis bacteria. Carpet anemone methanol extract (40 mg/ml) cause a growth inhibitory effect on E. coli, but did not have a deleterious effect. Starfish methanol extract showed the highest growth inhibitory effect on B. subtilis and created a growth inhibitory circle zoon (12.1±0.8 mm diameter). The results showed that the methanolic extract of the starfish has higher growth inhibitory effect on the two studied bacterial species. In conclusion, methanolic extracts of the starfish were effective in inhibiting the growth of Bacillus subtilis bacteria and methanolic extract of carpet anemone in inhibiting E. coli bacteria growth.
    Description: Published
    Keywords: Sarcophyton spp. ; Antibacterial ; Agar ; Bioactive ; Extraction ; Growth
    Repository Name: AquaDocs
    Type: Journal Contribution , Refereed
    Format: pp.195-205
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  • 3
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    In:  http://aquaticcommons.org/id/eprint/22077 | 18721 | 2018-02-01 09:52:46 | 22077 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-06-25
    Description: Sponges are the most primitive of the multicellular, These organisms don’t have any mechanical defense system, so their early appearance in evolution has given them a lot of time for the development of advanced secondary metabolites as chemical defense system. Sponges have the potential to provide drugs from chemical components against diseases. In this investigation the sponge samples, which it is Dysidea pallescens, were collected at depth of 15- 20 meter, from locations on the coastline of Island Hengam in Persian Gulf of Iran. For identifying natural components, methanol and diethyl ether were used as extraction solvents, after removal of the solvents; the in vitro cytotoxic activity was identified. In vitro cytotoxicity screening, by XTT assay, against KB/ C152 and HUT-78/ C185 cell line, was conducted in this study in 1 - 500 µg/ml. IC50 for diethyl ether and methanol extract was 200 µg/ml in HUT-78, IC50 for diethyl ether extract was 325µg/ml and methanol extract 325µg/ml in KB.
    Keywords: Biology ; Chemistry ; Environment ; Fisheries ; Pollution ; Sponge ; Cytotoxic ; Methanol extract ; Diethyl ether extract ; Hengam Island ; Persian Gulf ; Iran ; Dysidea pallescens
    Repository Name: AquaDocs
    Type: article , TRUE
    Format: application/pdf
    Format: application/pdf
    Format: 1-8
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  • 4
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25642 | 18721 | 2018-10-14 02:40:17 | 25642 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: Padina boergesenii is one of the most abundant brown algae distributed in the north of Persian Gulf and Oman Sea. In this study after sampling and preparation of Padina boergesenii by Chroform-Etanol (3-1) solvent and by Methanol has been extract. Separation and purification of the compounds was carried out using thin layer, general and inverse column chromatography, Cephadex and high-performance liquid chromatography (HPLC). Structural elucidation of the constituents was based on the data obtained from H-NMR, 13C-NMR, HSQC, HMBC, DEPT and Cephadex LH-20. The steroids compounds separated from above alga were identified as 22dehydrocholesterol (1), cholesterol (2), fucosterol (3), β-sitosterol (4), stigmasterol (5), ostreasterol (6) and two epimer of hyroxyestrol(7), based on their spectral data and from comparison with those previously reported in the literature.
    Keywords: Biology ; Chemistry ; Iran ; Oman Sea ; Persian Gulf ; Brown Algae ; Padina boergesenii ; Steroids compounds ; Extraction ; Purification ; Identification ; Amount verification ; Sargassum glaucescens ; Algae ; Abundant ; Sampling ; Chromatography ; Cephadex ; Dehydrocholesterol ; Cholesterol ; Fucosterol ; β-sitosterol ; Stigmasterol ; Ostreasterol
    Repository Name: AquaDocs
    Type: monograph
    Format: application/pdf
    Format: application/pdf
    Format: 42
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  • 5
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    In:  http://aquaticcommons.org/id/eprint/22011 | 18721 | 2018-01-22 11:09:49 | 22011 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-06
    Description: In recent years efforts to find bioactive compounds from live organisms especially marine animals have been increased. In the present study, the anticancer and cytotoxic effects of sea cucumber body walls (Holothuria leucospilota) were investigated. For this purpose, sea cucumbers were collected from Larak Island at depths of 10 to 30 m and extraction process was done with methanol and diethyl ether solvent which then concentrated by rotary evaporator (40℃) following lyophilization with vacuum freeze dryer. XTT method was used to investigate anticancer and cytotoxic effects of body wall extracts. The results showed that the methanolic extract could prevent proliferation of human oral epidermoid carcinoma cells (KB) at concentrations of 100 and 500 μg/ml. The diethyl etheric extract also could prevent proliferation of KB at 500 μg/ml concentration. Overall result showed that sea cucumber body wall had a strong cytotoxic effect on normal cell line (Human embryonic kidney cell [HEK]) which can be used as potent cytotoxic material. However these extracts did not show significant therapeutic value against KB cells.
    Keywords: Biology ; Fisheries ; Health ; Sea cucumber ; body wall ; methanolic extract ; diethyl etheric extract ; cytotoxic ; anti-cancer ; Holothuria leucospilota ; marine ; Iran
    Repository Name: AquaDocs
    Type: article , TRUE
    Format: application/pdf
    Format: application/pdf
    Format: 11-20
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  • 6
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    In:  http://aquaticcommons.org/id/eprint/22727 | 18721 | 2018-05-17 20:14:07 | 22727 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-09
    Description: Sea cucumber is presented as a potential marine source of antimicrobial compounds. The purpose of this study is to evaluate antifungal effects of sea cucumber, Holothuria leucospilota, extracts on Aspergillus niger and Candida albicans. Methanol and chloroform extracts of the body wall, gonad and intestine of sea cucumber, H. leucospilota, collected from Persian Gulf, were evaluated for their antifungal activity against A. niger and C. albicans. The activity was determined using serial dilution method. Antifungal activity, minimum inhibitory concentration and minimum fungicidal concentration were evaluated by the different concentrations. Results showed that each of the extracts had antifungal effect at specified concentrations on the A. niger. All examined concentrations of Gonad methanol and intestine chloroform extracts had no inhibitory effect on C. albicans. Chloroform extracts of gonad and intestine had more fungicidal effect against A. niger compared with C. albicans. Gonad chloroform extracts showed more fungicidal effect on the C. albicans at concentrations of 2.5, 5 and 10 mg/ml. Sea cucumber extract can be considered as an antifungal agent in various industries such as medicine and pharmaceutical industry.
    Keywords: Biology ; Fisheries ; Sea cucumber extract ; Persian Gulf ; Antifungal activity ; Inhibitory effect ; Biology ; physiology ; Iran
    Repository Name: AquaDocs
    Type: article , TRUE
    Format: application/pdf
    Format: application/pdf
    Format: 785-795
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  • 7
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    In:  http://aquaticcommons.org/id/eprint/22730 | 18721 | 2018-05-17 23:49:11 | 22730 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-09
    Description: Sponges, which constitute the phylum Porifera, are the most primitive of the multicellular animals, among all marine organisms screened. Marine sponges produce the largest number of structurally diversified natural products. In this study we investigated in vitro antimicrobial activity of Ircinia mutans collected from the Kish Island in the Persian Gulf against strains of bacteria Escherichia coli (ATCC 15224), Pseudomonas aeruginosa (ATCC 25619), Staphylococcus aureus (ATCC 1764), and Bacillus subtilis spizizenii (ATCC 6633). Diethyl etter, methanol and aqueous extracts of sponge were evaluated by using the Bacterial Broth Dilution Method. The results showed that the aqueous extracts didn’t have any antibacterial activity. Minimum Inhibitory Concentrations (MIC) of the winter diethyl etter extract was 2 mg/ml for E.coli and 20 mg/ml for P. aeruginosa, whereas the summer diethyl etter extract and both of methanol extracts did not show any activity. The MIC and MBC (Minimum Bacterial Concentration) of summer diethyl etter extracts were 2 mg/ml and 3mg/ml against S. aureus; and 5mg/ml and 10mg/ml when tested on B. subtilis. The MIC and MBC of winter diethyl etter extracts were measured as 1.5 mg/ml and 2mg/ml against S. aurous; and 5mg/ml and 10mg/ml when examined on B. subtilis. Summer and winter methanol and aqueous extracts of I. mutans did not show any activity against these bacteria. Therefore secondary metabolite solutions in diethyl etter contain components with antibacterial properties and can be used as antibiotics products.
    Keywords: Biology ; Fisheries ; Sponge ; Secondary metabolites ; Antibacterial activity ; Kish Island ; Persian Gulf ; Biology ; Physiology ; Iran ; Pseudomonas aeruginosa ; Escherichia coli ; Bacillus subtilis spizizenii
    Repository Name: AquaDocs
    Type: article , TRUE
    Format: application/pdf
    Format: application/pdf
    Format: 823-833
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  • 8
    Publication Date: 2021-07-16
    Description: This study was determined some important extracted materials, Such as : Alginates of sodium & calcium and alginic acid in three species of brown seaweeds (Sargassum illicifolium , Cystoseira indica and Nizimuddinia zanardinii) during in different season in 1387 in coastal erea of Sistan and Baluochestan Provience. At First seaweeds sample were colected from sea beach and transferd to lab, so washed in fresh water and dried in the sun. So were under Alkaloid extraction with sodium carbonate (5 Persent), that were obtined fluid after filteration. The sodium alginate fibers extracted from fluid were combined by Alcohol. The calacium alginate fibers were deposited by calacium chlorids (5%) and Alginic acid resulted with chloridric acid (1N). The extraction yield of sodium alginate, calacium alginate and alginic acid in different samples of seaweeds with following Explaination: The presentage we have got, can be said , the present average of sodium alginate at Sargassum in Spring season in three areas (Chabahar, Tang and Pasabandar ) Respectively: 28.4±2 , 28.9±2 , 27.2±1.9 present, the present average of sodium alginate at Cystoseira in Spring in three areas at above mentioned goes in this way: 19.7±1.4, 18.6±1.4, 19.2±1.3 present and the present average of sodium alginate at Nizimuddinia in Spring were 23.4±1.5, 23.8±1.5 , 20.6±1.5 in these three areas. The present average of calacium alginate at Sargassum in Spring season in three areas (Chabahar, Tang and Pasabandar ): 33.7±0.5, 33.7±0.8, 33.1±1 present, the present average of calacium alginate at Cystoseira in Spring in three areas at above mentioned goes in this way: 28.8±1.5, 27.4±1, 27.4±1.5 present and the present average of calacium alginate at Nizimuddinia in Spring were 35.9±2.8, 23.9±1.5 , 35.9±2.8 in these three areas. The present average of Alginic acid at Sargassum in Spring season in three areas (Chabahar, Tang and Pasabandar ): 19.2±1.6 , 25.6±1.7 , 18.4±1.1 present, the present average of Alginic acid at Cystoseira in Spring in three areas at above mentioned goes in this way: 16.8±1.2, 15.8±1.1, 16.4±1 present and the present average of Alginic acid at Nizimuddinia in Spring were 19.2±1.6, 19.6±1.7 , 18.4±1.5 in these three areas. The present average of sodium alginate at Sargassum in Summer season in three areas (Chabahar, Tang and Pasabandar ) Respectively: 20.8±1, 21±1, 27.8±1.9 present, the present average of sodium alginate at Cystoseira in Summer in three areas at above mentioned goes in this way: 21±1.1, 13.2±1, 14.1±0.7 present and the present average of sodium alginate at Nizimuddinia in Summer were 25.3±2.3, 16.7±2.1, 15.3±1.3 in these three areas. The present average of calacium alginate at Sargassum in Summer season in three areas (Chabahar, Tang and Pasabandar): 29.5±2.1 , 29.7±2 , 28.3±2.2 present, the present average of calacium alginate at Cystoseira in Summer in three areas at above mentioned goes in this way: 21±1.1, 20.2±0.9, 20±0.7 present and the present average of calacium alginate at Nizimuddinia in Summer were 25.3±2.3, 29.7±2 , 23.1±1.5 in these three areas. The present average of Alginic acid at Sargassum in Summer season in three areas (Chabahar, Tang and Pasabandar): 19.5±0.7 , 19.7±0.6 , 18.7±0.7 present, the present average of Alginic acid at Cystoseira in Summer in three areas at above mentioned goes in this way: 21±1.1, 11.5±1.4, 11.1±1.5 present and the present average of Alginic acid at Nizimuddinia in Summer were 14.8±1.3, 15.3±1.3 , 14.6±1 in these three areas. The present average of sodium alginate at Sargassum in Autumn season in three areas (Chabahar, Tang and Pasabandar) Respectively: 31.5±4.3, 31.6±4.8, 29.4±4.8 present, the present average of sodium alginate at Cystoseira in Autumn in three areas at above mentioned goes in this way: 23±2.7, 21.4±2.8, 21.4±2.7 present and the present average of sodium alginate at Nizimuddinia in Autumn were 25.8±2.2, 26.4±2.1 , 23.4±1.9 in these three areas. The present average of calacium alginate at Sargassum in Autumn season in three areas (Chabahar, Tang and Pasabandar): 43.3±1.6, 42.8±2.7, 41.7±2.9 present, the present average of calacium alginate at Cystoseira in Autumn in three areas at above mentioned goes in this way: 34.3±5.9, 32.4±5.5, 30.9±5.2 present and the present average of calacium alginate at Nizimuddinia in Autumn were 50±7.1, 50.2±7.5, 45.6±5.2 in these three areas. The present average of Alginic acid at Sargassum in Autumn season in three areas (Chabahar, Tang and Pasabandar): 28±3.8, 28±3.5, 26±3.9 present, the present average of Alginic acid at Cystoseira in Autumn in three areas at above mentioned goes in this way: 20.2±1.8, 19.2±2.1, 18.7±1.6 present and the present average of Alginic acid at Nizimuddinia in Autumn were 25.9±3, 26.1±2.9, 24.6±3.6 in these three areas. The present average of sodium alginate at Sargassum in Winter season in three areas (Chabahar, Tang and Pasabandar) Respectively: 35±1.6, 35.2±1.8 , 33.8±1.9 present, the present average of sodium alginate at Cystoseira in Winter in three areas at above mentioned goes in this way: 24.1±2, 22.9±1.2, 23.3±0.7 present and the present average of sodium alginate at Nizimuddinia in Winter were 28.2±1.3, 29.2±1.7, 26.2±1.8 in these three areas. The present average of calacium alginate at Sargassum in Winter season in three areas (Chabahar, Tang and Pasabandar): 45.6±1.9, 45±2, 44.6±2.5 present, the present average of calacium alginate at Cystoseira in Winter in three areas at above mentioned goes in this way: 38.2±2.8, 35.9±3.4, 35.6±3.5 present and the present average of calacium alginate at Nizimuddinia in Winter were 56.4±3.5, 56±3.5, 51.8±2.5 in these three areas. The present average of Alginic acid at Sargassum in winter season in three areas (Chabahar, Tang and Pasabandar): 31.8±1.2, 32.1±0.9, 30.9±1.2 present, the present average of Alginic acid at Cystoseira in Winter in three areas at above mentioned goes in this way: 21.1±1.2, 20.4±1.4, 19.3±1.4 present and the present average of Alginic acid at Nizimuddinia in Winter were 26.4±2.1, 27±2.2, 24.6±2.2 in these three areas. The more amount of calacium alginate in Autumn can be found at Nizimuddinia in Tang area , wite amount of 50.2 present and the lates amount of Alginic acid at Cystoseira can be found in Summer with 11.1 present. If the Alginic acid become more than 20 present, it can be economical, that it can be showed more than 20 present in Autumn and Winter. According to this results, One way ANOVA showed that average of Alginates were not similar and significant differences ( P〈0.05 ) between species.
    Keywords: Biology ; Iran ; Sistan and Baluochestan Provience ; Chabahar ; Tang ; Alginic acid ; Species ; Brown seaweeds ; Sargassum illicifolium ; Cystoseira indica ; Nizimuddinia zanardinii ; Chloridric acid ; ANOVA ; Sodium ; Calcium ; Seaweeds
    Repository Name: AquaDocs
    Type: monograph
    Format: application/pdf
    Format: application/pdf
    Format: 74
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  • 9
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25503 | 18721 | 2018-10-05 07:54:25 | 25503 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: Specialists in Marine biotechnology using marine biological engineering and scientific principles to develop natural products and regarded services. One of the most important applications of this new branch is produce the natural products such as enzymes, natural colors, vaccines and drugs and etc. which produced from marine living organisms. These products are used in various industries such as pharmaceutical, food or health industry. In the current study, we try to remark the application of marine biological products in the northern (Caspian Sea), southern (Persian Gulf and Oman Sea) or internal waters of Iran into six categories, including aquaculture, pharmaceutical industry, food, healthcare and cosmetic industries. For this purpose, the required information will be collected using investigations which conducted inside and also outside of our country by reviewing and analysis of derived data by attention to our economical facilities for national self-sufficiency in production. Applied research projects will be introduced in three parts including research, technology development and pilot plant as well. Hope that using this strategic document will be effective and practical to production of biological products from marine resources until 2025.
    Keywords: Management ; Iran ; Enzymes ; Marine ; Biological ; Biotechnology ; Aquatic
    Repository Name: AquaDocs
    Type: monograph
    Format: application/pdf
    Format: application/pdf
    Format: 131
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  • 10
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25232 | 18721 | 2018-09-06 06:16:53 | 25232 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: Fish culture has developed in Yazd province of Iran since recent decade and the rainbow trout, Oncorhynchus mykiss is the most important fisheries product in the area. There is a few information about carcass composition of cultured fish in Iran. Rainbow trout samples were collected from 6 fish brackish and fresh water fish farms of Yazd province during February 2007. Fish muscle samples were freezed in -30 C after being homogenized, till laboratory analyses. Proximate composition of samples contained 17.05-18.53% crude protein, 2.35-5.13% total fat, 1.31-1.7% ash and 74.18-77.05% wet. Saturated fatty acids including palmitic acid (14.06-19.60%), stearic acid (6.44-6.1%), myristic acid (1.29-5.66%) and lauric acid (0.13-2.76), also unsaturated fatty acids oleic (30.24-40.14%) and linoleic (21.18-38.85%) were extracted from muscle tissue of fish of different farms, using GC. Other unsaturated fatty acids including -linolenic acid, EPA and DHA had low concentrations (1-3%) in samples. Vitamin E level was 4.33-94.34 g/100gr. Concentration of minerals in homogenated muscle tissues were as: Na 822.58-1052.78ppm, K 12272.75-13715.25ppm, Ca 104.75-171.03ppm, Fe 19-40ppm, Cu 0.044- 1.01ppb, Zn 7.02-12.47ppb, Cd 0.879-1.099ppb and Cr 1.895-3.86ppb. Heavy metal concentrations were lower than permitted values.
    Keywords: Aquaculture ; Iran ; Yazd province ; Oncorhynchus mykiss ; Rainbow trout ; Fish culture ; Tissues ; Brackish water ; Muscle
    Repository Name: AquaDocs
    Type: monograph
    Format: application/pdf
    Format: application/pdf
    Format: 80
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