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  • Fisheries  (38)
  • 2020-2022  (38)
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  • 1
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    Genetic variability and differentiation of common Kilka fish (Clupeonella cultriventris Nordmann, 1840) in the southern coasts of Caspian Sea (2021)
    In:  http://aquaticcommons.org/id/eprint/21944 | 18721 | 2018-01-18 08:55:31 | 21944 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-05
    Description: A total of 120 samples of adult common Kilka fish (Clupeonella cultriventris) were collected during spring and summer from the southern coasts of Caspian Sea (Bandar Anzali and Babolsar). Fifteen sets of microsatellite primers were developed from Clupeidae being tested on genomic DNA of common Kilka. Allele frequency, observed and expected heterozygosity, FST, RST, FIS index were determined. Five primer sets as polymorphic loci were used to analyze the genetic variation in adults of the common Kilka population. Results revealed that average alleles per locus was 13.1 (range 5 to 22 alleles per locus in regions, Ne=9.5). All sampled regions contained private alleles. Average observed and expected heterozygosity was 0.348 and 0.877, respectively. Deviations from Hardy Weinberg equilibrium were observed in most cases. FST, RST and gene flow estimates in AMOVA and the genetic distance between populations indicated that the genetic difference among the studied populations was pronounced. The data generated in this study provide primary information on the genetic variation and differentiation in populations of Caspian common Kilka.
    Keywords: Biology ; Fisheries ; Population ; genetic ; Microsatellite ; Clupeidae ; Common kilka ; Clupeonella cultriventris ; Caspian Sea ; Iran
    Repository Name: AquaDocs
    Type: article , TRUE
    Format: application/pdf
    Format: application/pdf
    Format: 139-148
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  • 2
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    Genetic relationships of Iranian coastline ship sturgeon (Acipenser nudiventris) samples and Ural population based on microsatellite DNA (2021)
    In:  http://aquaticcommons.org/id/eprint/22458 | 18721 | 2018-04-09 17:47:06 | 22458 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-05
    Description: Genetic relationships among 96 specimens of ship sturgeon, Acipenser nudiventris, collected from six locations along the Iranian coastline as well as 8 specimens from northern population (Ural River) were analysed by the restricted maximum likelihood method, as well as two distance analysis method (Nei's and Cavalli-Sforza's distance) of gene frequencies. We have used four SSR markers that produced three polymorphic and two monomorphic loci. Overall results showed that Iranian coastline samples form a monophyletic group (clade) which was different from the northern (Ural) samples. Iranian coastline samples are divided into two groups (clades): Anzali-Kiashahr clade and Sefidrud-Babolsar-Noushahr-Gorgan clade. Bootstrapping test showed monophyleticity of Sefidrud, Babolsar, Noushahr, Gorgan with high (Bootstrap support=93) and Anzali-Kiashahr with middle (Bootstrap support=65) confirmation. Topology of reconstructed trees was in correspondence with geographical distributions of samples.
    Keywords: Biology ; Fisheries ; Ship sturgeon ; Acipenser nudiventris ; Genetic relationships ; Microsatellite DNA ; Iran
    Repository Name: AquaDocs
    Type: article , TRUE
    Format: application/pdf
    Format: application/pdf
    Format: 229-240
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  • 3
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    Application of microsatellite markers to determine populations of the Persian sturgeon (Acipenser persicus) in the south of Caspian Sea (2021)
    In:  http://aquaticcommons.org/id/eprint/22465 | 18721 | 2018-04-10 21:21:52 | 22465 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-06
    Description: The objective of this study was to analyse the population genetic structure of the Persian sturgeon (Acipenser persicus) in Sefidrud and Gorganrud rivers watershed based on the characterization of microsatellite markers during 2006 - 2008. 100 samples of Persian sturgeon were collected from two regions. Four microsatellite loci (Ls68, Spl168, Spl173 and Afu68) were analyzed for the molecular characterization of this species which resulted in polymorphic patterns. DNA bands were analysed using Biocapt and GenAlex software package. A total of 109 alleles were observed of which the maximum number of alleles (17) were found in Spl168 locus which belonged to sturgeons from Sefidrud river's watershed and the minimum number of alleles (10) in Ls68 locus belonging to the sturgeons from Gorganrud river's watershed. Results of microsatellite analysis revealed that the differences between samples of two regions were not statistically significant (p〉0.05), neither for the average number of alleles per locus nor for observed heterozygosities. The calculated Fst and Rst between two regions was 0.07 and 0.17 showing that the genetic difference was significant (p〈 0.01). Samples from Sefidrud river's watershed in Spl173, Afu68 and Spl168 loci and samples of other regions in Afu68 and Spl168 loci were at Hardy-Weinberg equation. The genetic distance was calculated as 0.4 which represents a significant genetic difference between samples of two studied areas. In conclusion, this study suggests that the Persian sturgeons in two regions of the southern part of the Caspian Sea are genetically differentiated, therefore fisheries management of these unique stocks for restocking and conservation of gene pools is highly recommended.
    Description: Article includes abstract in Farsi on last page.
    Keywords: Biology ; Fisheries ; Persian sturgeon ; Acipenser persicus ; Caspian Sea ; Microsatellite ; Genetic structure ; Population genetic ; Iran
    Repository Name: AquaDocs
    Type: article , TRUE
    Format: application/pdf
    Format: application/pdf
    Format: 596-606
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  • 4
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    Karyotype analysis in white bream (Blicca bjoerkna transcaucasica) from north coast of Iran (2021)
    In:  http://aquaticcommons.org/id/eprint/22390 | 18721 | 2018-04-03 13:13:58 | 22390 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-04
    Description: Preparation of chromosome spreads and karyotype analysis in Blicca bjoerkna transcaucasica were carried out using 0.01% solution of colchicines and Phytohaemagglutinin (PHA) (20 µg g-1 body weight). The gill and kidney tissues were collected and let to stand in a hypotonic solution of 0.075 M KCl and then treated with a fixative (Carnoy's solution) in three steps. The chromosomes spreads were then stained with 5% Giemsa solution for 20 min and examined under a light microscope. Appropriate metaphase plates were photographed in order to prepare karyotype. The size of the chromosomes (short and long arms), relative length of chromosomes and centromere index were calculated. Chromosome spreads from gill tissue cultures which were colchicine treated with PHA, had a well defined size, shape and number of chromosomes for karyotype analysis. Based on the 76 metaphase plates studied, chromosome count in 59 metaphase plates was 2n=49.74±0.25. By arranging homologous chromosomes beside each other the chromosome formula was calculated as 6 pairs of Metacentric, 10 pairs of Sub-Metacentric and 9 pairs of Sub-Telocentric (2n=6M+10+Sm+9St) and the chromosome arm number (NF) was 100. The largest chromosome in this species was a pair of metacentric chromosomes. On the basis of the number and type of chromosomes, the karyotype obtained for this species conformed to the findings of other researchers, but the chromosome formula was different, which could be attributed to the existence of different populations for this species.
    Keywords: Biology ; Fisheries ; Blicca bjoerkna transcaucasica ; Phytohaemagglutinin ; Colchicines ; Karyotype ; Iran
    Repository Name: AquaDocs
    Type: article , TRUE
    Format: application/pdf
    Format: application/pdf
    Format: 454-463
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  • 5
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    Persian sturgeon growth hormone elaboration and purification (2021)
    In:  http://aquaticcommons.org/id/eprint/22837 | 18721 | 2018-05-25 22:34:14 | 22837 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-10
    Description: In this study Escherichia coli DE3 containing expression vector (pET21a) with cloned Persian sturgeon growth hormone (psGH) gene was grown in 10 mL LB broth on a 150 rpm shaker, at the temperature of 37 °C. At the late log phase (determined by OD standard curve) 100 µL isopropyl β-D-1-thiogalactopyranoside (IPTG) was added for induction of GH synthesis. Samples were taken every 2 hours and after bacterial cells lysis crude extracts with recombinant proteins inclusion bodies (IB) were loaded on 15% SDS-PAGE gel. Thenafter staining, comparative concentrations of rpsGH were measured by densitometric scanning of sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) gel it was more than 90 %. The maximum yield of GH was observed after 4 hours of growth. To recover active psGH from inclusion bodies we used imidozole to obtain most of the total recombinant protein in the soluble fraction. Purification of 6xhisN tag recombinant psGH has been performed using affinity chromatography where nickel was bound to an agarose bead by chelation using NTA (nitrilotriacetic acid) beads. The overall yield of the purified monomeric psGH was approximately 50% of the initial IB proteins. The purification manipulations including IB isolation and solubilisation, protein refolding by dialyze and affinity chromatography ensure yields of biologically active psGH up to 30%. This study shows that, the affinity chromatography is a powerful and very specific method for recombinant proteins purification of psGH.
    Keywords: Biology ; Chemistry ; Fisheries ; Persian sturgeon ; Growth hormone ; Purification ; Affinity chromatography ; Biotechnology ; Iran
    Repository Name: AquaDocs
    Type: article , TRUE
    Format: application/pdf
    Format: application/pdf
    Format: 1053-1060
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  • 6
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    Failure of PCR-RAPD technique to differentiate sex in Mahisefied (Rutilus frisii kutum) from the south Caspian Sea (2021)
    In:  http://aquaticcommons.org/id/eprint/21699 | 18721 | 2017-11-27 12:11:39 | 21699 | University of Guilan, Faculty of Natural Resources, Iran
    Details
    Publication Date: 2021-06-28
    Description: In order to identify the sex marker in Mahisefied, Rutilus frisii kutum, samples from 5 male and 5 female fish were collected from the south Caspian Sea. Polymerase chain reaction random amplified polymorphic DNA (PCR-RAPD) was performed using 124 primer sets. All bands were numbered using 1 and 0 scores corresponding to the presence or absence of bands, respectively and data were analyzed using RAPDPLOT program. Results indicated that 44 sets of primers did not show any flanking site and produced no bands, while the remaining 80 produced sharp and visible bands on polyacrylamid gel. In total, 1600 bands were scored. However, none of the bands corresponded to either the male or female fish.According to the results it has been concluded that RAPD technique failed to detect sex and cannot be considered as a robust molecular tool for sex differentiation in the studied fish. The reason may be the absence of sex chromosomes in this species or that the genes corresponding to sex differentiation are spread on different autosomal chromosomes with interaction of some environmental factors.
    Keywords: Biology ; Fisheries ; failure ; PCR-RAPD ; technique ; differentiate ; sex ; mahisefied ; Rutilus frisii kutum ; Caspian Sea ; iran ; DNA
    Repository Name: AquaDocs
    Type: article , TRUE
    Format: application/pdf
    Format: application/pdf
    Format: 235-242
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  • 7
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    Induction of triploidy in grass carp Ctenopharyngodon idella Valenciennes, 1844: comparison of cold & heat shocks (2021)
    In:  http://aquaticcommons.org/id/eprint/21714 | 18721 | 2017-11-28 08:30:48 | 21714 | University of Guilan, Faculty of Natural Resources, Iran
    Details
    Publication Date: 2021-06-29
    Description: Triploidy in grass carp, Ctenopharyngodon idella Valenciennes, 1844, was induced on fertilized eggs to compare cold and heat shocks. Two simplified methods explained for verification of triploidy in grass carp. The cold shock (7 ˚C) was given in three treatments for 30 min starting 2.0, 2.5 and 4.0 min after fertilization. In cold shock, the start point (2.0 min after fertilization) showed the highest rate of triploidy (60.9%). Heat shocks were given at 38 ˚C, 40 ˚C and 42 ˚C, at 4.0 min after fertilization and lasted for 1.0 min. Produced larvae using heat shock 38 ˚C showed 10.8% triploidy, but no signs of triploidy were seen in other heat shock treatments. Verification of triploidy in grass carp was carried out using karyotyping and measurment of erythrocytes surface area and volume in fingerlings. Ratio of erythrocytes dimention and the size of their nuclei in triploids to diploids was 2.35 and 1.80, respectively. Comparison of results obtained from the application of cold and heat shocks indicated that cold shocks are more effective than heat shocks in the induction of triploidy in grass carp.
    Keywords: Biology ; Fisheries ; induction ; triploidy ; grass carp ; Ctenopharyngodon idella ; comparison ; cold ; heat shocks ; triploidy ; Iran
    Repository Name: AquaDocs
    Type: article , TRUE
    Format: application/pdf
    Format: application/pdf
    Format: 195-204
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  • 8
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    Searching the genome of beluga (Huso huso) for sex markers based on targeted bulked segregant analysis (BSA) (2021)
    In:  http://aquaticcommons.org/id/eprint/21740 | 18721 | 2017-11-28 09:11:49 | 21740 | University of Guilan, Faculty of Natural Resources, Iran
    Details
    Publication Date: 2021-06-30
    Description: In sturgeon aquaculture, where the main purpose is caviar production, a reliable method is needed to separate fish according to gender. Currently, due to the lack of external sexual dimorphism, the fish are sexed by an invasive surgical examination of the gonads. Development of a non-invasive procedure for sexing fish based on genetic markers is of special interest. In the present study we employed Bulked Segregant Analysis (BSA) methodology to search for DNA markers associated with the sex of the beluga sturgeon (Huso huso). DNA bulks (male and female) were created by combining equal amounts of genomic DNA from 10 fish of both sexes. A total of 101 decamer primers associated with the sex-specific sequences in non-sturgeon species was used for targeted screening of the bulks, resulting in 2846 bands that all of them were present in both sexes. Our results showed that sex chromosomes are weakly differentiated in the sturgeon genome and comprised sequences not complementary to the sex-specific primers in non-sturgeon species.
    Keywords: Biology ; Fisheries ; genome ; beluga ; huso huso ; sex ; markers ; segregant ; DNA ; species ; Iran
    Repository Name: AquaDocs
    Type: article , TRUE
    Format: application/pdf
    Format: application/pdf
    Format: 185-195
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  • 9
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    Karyology study of Spirlin (Alburnoides bipunctatus) in Zabol region (2021)
    In:  http://aquaticcommons.org/id/eprint/21957 | 18721 | 2018-01-18 09:58:06 | 21957 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-05
    Description: The chromosomal spread and karyotype of Spirlin (Alburnoides bipunctatus) from Zabol region were identified using tissue squashing techniques with injection of 0.5ml/100g body weight of 0.01% Colchicines solution in fish fingerlings. Kidney and gill tissues were extracted and chopped in KCl 0.075M for 30 min and fixed in Carnoy solution in 3 stages. The chromosomal spreads were stained in 20% Gimsa for 20 min. From 34 chromosomal spread counts, the results showed diploid chromosome number of this species 2n=50. Karyotype composed of 9 metacentric, 14 submetacentric and 2 acrocentric or subtelocentric chromosome pairs, and the number of chromosome arms (NF) was determined as NF=96.
    Keywords: Biology ; Fisheries ; Zabol ; Karyotype ; Spirlin ; Alburnoides bipunctatus ; Iran ; gill ; tissues ; species ; chromosomal
    Repository Name: AquaDocs
    Type: article , TRUE
    Format: application/pdf
    Format: application/pdf
    Format: 137-146
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  • 10
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    Population genetic structure of Persian sturgeon (Acipenser persicus) between South Caspian Sea and Sefidrud River using DNA sequencing method (2021)
    In:  http://aquaticcommons.org/id/eprint/22001 | 18721 | 2018-01-21 12:06:13 | 22001 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-06
    Description: The goal of this study was to analyse the population genetic structure of the Persian sturgeon (Acipenser persicus) between South Caspian Sea and Sefidrud River with mtDNA control region (Dloop gene) and DNA sequencing method during 2010 – 2012 sturgeon stock assessment project. Fish speciemns were collected by bottom trawl net. Extraction of DNA, PCR and DNA sequencing were carried out. Diversity index, the gamma distribution shape parameter for the rate heterogeneity among sites and nucleotide sequence, Fst index, exact test, the historical demographic pattern using neutrality tests and mismatch distribution analysis (D test of Tajima and Fs test of Fu) were analysed. Thirteen haplotypes were obtained, average (±SD) for haplotype diversity was 0.961 ± 0.101, nucleotide diversity was 0.038 ± 0.015, the gamma distribution shape parameter was 0.19, Fst index revealed little genetic structure between populations and the significant Fst value was seen by 10000 permutation only between Sefidrud River and Other Areas (P≤ 0.05) and was confirmed by exact test of population differentiation. Mismatch distribution for Acipenser persicus appeared to be unimodal, which closely matched the expected distributions under the sudden expansion model and supported by the low Harpending’s Raggedness index (0.061). Tajima’s D and Fu’s Fs statistics were -0.84 and - 0.220, respectively, and was not significant. The results of this study showed that the population of Acipenser persicus in Sefidrud River were genetically differentiated from South Caspian Sea and three other areas represented a single panmictic populations. Therefore, fisheries managements of this valuable species should be directed towards conservation of gene pools and increasing different populations.
    Keywords: Biology ; Fisheries ; Acipenser persicus ; population ; genetic ; structure ; South Caspian Sea ; DNA sequencing ; Persian sturgeon ; Sefidrud River ; Caspian Sea ; method ; Iran ; parameter ; heterogeneity ; populations ; fisheries
    Repository Name: AquaDocs
    Type: article , TRUE
    Format: application/pdf
    Format: application/pdf
    Format: 11-20
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