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  • Brackish  (5)
  • 2020-2022  (5)
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  • 1
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    Effect of different dietary protein to energy ratios (P/E) on growth performance and body composition of farmed Persian sturgeon (Acipenser persicus) (2021)
    In:  http://aquaticcommons.org/id/eprint/23634 | 18721 | 2018-07-13 17:42:15 | 23634 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-14
    Description: To improve Persian sturgeon nutrition and determine its nutritional requirements, a growth trial was conducted to measure optimum dietary protein to energy ratio in Persian sturgeon sub-yearlings. Four diets: H (P/E=21.33mg/kJ), N (P/E=20.24mg/kJ), L (P/E=19.22 mg/kJ) and M (P/E=18.26mg/kJ) were used in the experiment. Triplicate groups of fish were kept in 500L fiberglass tanks under homogenous culture conditions (dissolved oxygen, light and water flow rate) and fed with the experimental diets. Fish were fed to satiation four times per day during 130 one fourth ¬ days of experiment when the average water temperature and dissolved oxygen were 12.82 plus or minus 1.48 degree C and 9.55 plus or minus 0.18 mg/l respectively. Fish in all treatments grew fast and efficiently utilized the diets. Growth rate, FE, GR, SGR and PER increased with decreasing dietary protein to energy ratios. A higher growth was observed in the fishes fed with lower P/E ratios. Food intake and fatness coefficient increased with increasing dietary energy and decreasing P/E ratios. There were no significant differences in feeding efficiency (FE) and protein efficiency ratio (PER) in fish fed with diet N (high carbohydrate and low lipid content) and diet L (low carbohydrate and high lipid content). We conclude that the Persian sturgeon is able to use lipids and carbohydrates as non-protein sources of energy. The results of the present study demonstrated that the protein content and P/E ratio for optimum growth and efficient protein utilization in Persian sturgeon is 40% and 18.3-19.2 mg protein/kJ energy, respectively.
    Keywords: Aquaculture ; Growth rate ; Biochemical composition ; Diet ; Energy ; Protein deficiency ; Dietary deficiencies ; Fish Culture ; Feed composition ; Food absorption ; Acipenser persicus ; Brackish ; Caspian Sea ; Iran
    Repository Name: AquaDocs
    Type: article , TRUE
    Format: application/pdf
    Format: application/pdf
    Format: 129-140
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  • 2
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    Karyology of pikeperch (Sander lucioperca)in the south Caspian Sea (2021)
    In:  http://aquaticcommons.org/id/eprint/23652 | 18721 | 2018-07-15 00:13:02 | 23652 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-14
    Description: Pikeperch (Sander lucioperca) is one of the economic fish species in the Caspian Sea, with no official report on its chromosome preparation and karyotype formula. For the metaphase preparation, 29 fry specimens of the fish weighting 10-25g each were used. Colchicine 0.01% at 0.7ml/100g of fish weight was injected in the muscular tissues. After 180 minutes, kidney and gill tissues were removed and hypotonized in KCL 0.075 M for 40 minutes, fixed with Carnoy mixture for 18-20 hours, homogenized and dropped on the slides, and stained with Giemsa 20% for 30 minutes. The prepared slides were swept by objective X100 light photomicroscope. The results showed that the chromosome number of this species is 2n = 48 and the chromosome arms is NF = 76. The karyotype formula of the species was found to be 2n = 1m + 13sm + 4st + 6a. Comparing the results of the study with those of other researchers showed that the pikeperch chromosome number in the South Caspian Sea is similar to the fish in other parts of the world but the type of chromosomes and karyotype formula is different.
    Keywords: Biology ; Chromosomes ; Economics ; Kidney ; Gills ; Colchicine ; Light microscopy ; Commercial species ; Karyotypes ; Sander lucioperca ; Metaphase ; potassium chloride ; Chromosome number ; Brackish
    Repository Name: AquaDocs
    Type: article , TRUE
    Format: application/pdf
    Format: application/pdf
    Format: 137-144
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  • 3
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    Investigation on genetic structure of Russian sturgeon (Acipenser gueldenstaedtii) populations of the north (Volga River) and south Caspian Sea (coasts of Iran and Turkmenistan) using microsatellite techniques (2021)
    In:  http://aquaticcommons.org/id/eprint/23680 | 18721 | 2018-07-18 06:38:41 | 23680 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-15
    Description: A total of 28 specimens of adult Russian sturgeon brood fish from the Volga River (Astrakhan, Russia) and 42 specimens from the south Caspian Sea (coastline of Iran and Turkmenistan) were collected. About 2g of fin tissue was stored in 96% ethyl alcohol and transferred to the genetic laboratory of the International Sturgeon Research Institute. Genomic DNA was extracted using phenol-chloroform method. The quality and quantity of DNA was assessed by Agarose gel electrophoresis and spectrophotometry. Polymerase Chain Reaction (PCR) was conducted using eight pairs of microsatellite primers and its products were electrophoresed using 6% polyacrylamide gel followed by silver nitrate staining. Allele sizes were measured in all populations, then genetic parameters were calculated using Gen Alex program and the phylogenetic relationship was determined and drawn using TFPGA program. A minimum of 10 and a maximum of 21 alleles were identified per locus and the observed heterozygosity ranged between 0.50-0.96 and the expected heterozygosity was 0.74-0.90 with an average of 0.68. Hardy-Weinberg equilibrium was observed at Ls-19, Ls-39 loci, but showed disequilibrium in other loci. FST index between Volga and South Caspian Sea samples was 0.031. The genetic similarity and distance was 0.661 and 0.414, respectively. Results of the present investigation indicate that there are no significant differences between the south Caspian Sea Russian sturgeon specimens.
    Keywords: Biology ; Population Genetics ; Genes ; Electrophoresis ; Fins ; DNA ; Genetic diversity ; Natural populations ; Phylogenetics ; Phylogeny ; Heterozygosity ; Migration ; Alcohols ; Spectrophotometry ; Genomics ; Silver nitrate ; Genetic structure ; Rivers ; Microsatellites ; Gel electrophoresis ; Polymerase chain reaction ; Primers ; Coasts ; Acipenser gueldenstaedtii ; Acipenser ; Brackish ; Caspian Sea ; Caspian Sea Turkmenistan ; Iran
    Repository Name: AquaDocs
    Type: article , TRUE
    Format: application/pdf
    Format: application/pdf
    Format: 69-80
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  • 4
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    Effects of dietary L-Ascorbyl-2-Polyphosphate as a source of vitamin C on growth indices in beluga sturgeon (Huso huso L.) (2021)
    In:  http://aquaticcommons.org/id/eprint/23736 | 18721 | 2018-07-21 17:18:02 | 23736 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-15
    Description: Six diets supplemented with 0, 100, 200, 400, 800, and 1600mg/kg L-Ascorbyl-2-Polyphosphate (APP) as vitamin C, were fed to juvenile beluga sturgeon in three replicates for 16 weeks. Fifty five fish with an average weight 38.1 plus or minus 0.5 g were distributed to each tank after adaptation to the experimental diets. Most of the growth performance indices at week 4 indicated significant differences (P〈0.05), except in CF and PER (P〈0.05). The survival was affected by dietary treatment at week 4 (P〈0.05). No deficiency sign was observed during the trial. Results of this study indicated that vitamin C can have remarkable effects on growth indices in different growth periods. Therefore, regarding the effects of vitamin C on the growth indices and in the fish weight range used in this study, 100-200 mg/kg vitamin C (APP) can be considered as the optimum level for rearing the fish.
    Keywords: Aquaculture ; Growth rate ; Vitamin C ; Feeding ; Juveniles ; Condition factor ; Huso huso ; Brackish ; Caspian Sea ; Iran
    Repository Name: AquaDocs
    Type: article , TRUE
    Format: application/pdf
    Format: application/pdf
    Format: 107-120
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  • 5
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    Population structure of Acipenser nudiventris in the south coast of Caspian Sea and Ural River using microsatellite method (2021)
    In:  http://aquaticcommons.org/id/eprint/23700 | 18721 | 2018-07-18 08:50:22 | 23700 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-15
    Description: Population structure of Ship sturgeon, Acipenser nudiventris, from the south coast of Caspian Sea and Ural River was investigated using Microsatellite method. For this reason, 73 specimens of the sturgeon were collected from five locations in two sampling regions the first consisted of Bandar Anzali, SefidRud River, Babolsar, and Gorgan, and the second was Ural River. Four SSR markers were used in this investigation, of which 5 loci produced DNA band, with three of them being polymorph. One primer showed two loci with one of them being polymorph and another was monomorphic). Average expected and observed heterozygosity was 0.86 and 0.75 respectively. Genetic variation was assessed through analysis of molecular variance (AMOVA) that indicated almost all of the variance in data namely %94 (P less than or equal to 0.03) was within locations.
    Keywords: Biology ; Population Genetics ; DNA ; Genetic diversity ; Population structure ; Genotypes ; Biopolymorphism ; Aquaculture techniques ; Fish ; Sturgeon ; Populations ; Sexual Reproduction ; Rivers ; Anadromous species ; Nucleotide sequence ; Induced breeding ; Primers ; Ships ; Sampling ; Coasts ; Acipenser ; Acipenser nudiventris ; Brackish ; Caspian Sea ; Ural R. Eurasia ; Caspian Sea Eurasia ; Iran
    Repository Name: AquaDocs
    Type: article , TRUE
    Format: application/pdf
    Format: application/pdf
    Format: 99-108
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