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  • Aquaculture  (25)
  • 2020-2022  (25)
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  • 1
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    In:  http://aquaticcommons.org/id/eprint/21728 | 18721 | 2017-11-28 08:45:40 | 21728 | University of Guilan, Faculty of Natural Resources, Iran
    Publication Date: 2021-06-29
    Description: The optimum dose of ultraviolet (UV) irradiation to produce gynogenetic Caspian salmon, Salmo trutta caspius, with emphasis on the "Hertwig effect" and photoreactivation (PR) were investigated. The sperm of Caspian salmon was irradiated with UV at 2010 ± 200 µw.cm-2 in different times including 0, 1, 3, 5, 8, 10, 15, 20, 25, 35 and 45 min and was allowed to fertilize normal ova; the fertilization, eyed and hatching rates were calculated to assess the performance. Using the irradiated sperm decreased the fertilization, eyed as well as hatching rates and the so-called "Hertwig effect" was observed, with the time-dependent decrease in the hatching rates at 0+ to 3 min irradiation, but better hatching rates were observed at more prolonged irradiation times. The best hatching rate was achieved at 25 min of UV irradiation; after that the survival rates rapidly declined to near zero. For PR studies, the semen was irradiated with UV (5, 30 and 120s) and untreated semen (0s) was used as control. Irradiated semen and/or fertilized eggs by treated semen were exposed to visible light (60 W) at a distance of 30 cm for 10 min; the eyed and hatching rates were measured. UV irradiation as low dose as 5s, significantly decreased the hatching rate (P 〈0.05). Semen and/or eggs illumination with visible light could not improve the survival rates (P 〉0.05). So, based on the results of this study, it was impossible to detect any PR mechanism in Caspian salmon.
    Keywords: Aquaculture ; Biology ; Salmo trutta caspius ; sperm ; fertilization ; capacity ; Caspian salmon ; ultraviolet ; irradiation ; Caspian sea ; iran ; photoreactivation ; gynogenetic
    Repository Name: AquaDocs
    Type: article , TRUE
    Format: application/pdf
    Format: application/pdf
    Format: 1-13
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  • 2
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    In:  http://aquaticcommons.org/id/eprint/23634 | 18721 | 2018-07-13 17:42:15 | 23634 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-14
    Description: To improve Persian sturgeon nutrition and determine its nutritional requirements, a growth trial was conducted to measure optimum dietary protein to energy ratio in Persian sturgeon sub-yearlings. Four diets: H (P/E=21.33mg/kJ), N (P/E=20.24mg/kJ), L (P/E=19.22 mg/kJ) and M (P/E=18.26mg/kJ) were used in the experiment. Triplicate groups of fish were kept in 500L fiberglass tanks under homogenous culture conditions (dissolved oxygen, light and water flow rate) and fed with the experimental diets. Fish were fed to satiation four times per day during 130 one fourth ¬ days of experiment when the average water temperature and dissolved oxygen were 12.82 plus or minus 1.48 degree C and 9.55 plus or minus 0.18 mg/l respectively. Fish in all treatments grew fast and efficiently utilized the diets. Growth rate, FE, GR, SGR and PER increased with decreasing dietary protein to energy ratios. A higher growth was observed in the fishes fed with lower P/E ratios. Food intake and fatness coefficient increased with increasing dietary energy and decreasing P/E ratios. There were no significant differences in feeding efficiency (FE) and protein efficiency ratio (PER) in fish fed with diet N (high carbohydrate and low lipid content) and diet L (low carbohydrate and high lipid content). We conclude that the Persian sturgeon is able to use lipids and carbohydrates as non-protein sources of energy. The results of the present study demonstrated that the protein content and P/E ratio for optimum growth and efficient protein utilization in Persian sturgeon is 40% and 18.3-19.2 mg protein/kJ energy, respectively.
    Keywords: Aquaculture ; Growth rate ; Biochemical composition ; Diet ; Energy ; Protein deficiency ; Dietary deficiencies ; Fish Culture ; Feed composition ; Food absorption ; Acipenser persicus ; Brackish ; Caspian Sea ; Iran
    Repository Name: AquaDocs
    Type: article , TRUE
    Format: application/pdf
    Format: application/pdf
    Format: 129-140
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  • 3
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    In:  http://aquaticcommons.org/id/eprint/23736 | 18721 | 2018-07-21 17:18:02 | 23736 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-15
    Description: Six diets supplemented with 0, 100, 200, 400, 800, and 1600mg/kg L-Ascorbyl-2-Polyphosphate (APP) as vitamin C, were fed to juvenile beluga sturgeon in three replicates for 16 weeks. Fifty five fish with an average weight 38.1 plus or minus 0.5 g were distributed to each tank after adaptation to the experimental diets. Most of the growth performance indices at week 4 indicated significant differences (P〈0.05), except in CF and PER (P〈0.05). The survival was affected by dietary treatment at week 4 (P〈0.05). No deficiency sign was observed during the trial. Results of this study indicated that vitamin C can have remarkable effects on growth indices in different growth periods. Therefore, regarding the effects of vitamin C on the growth indices and in the fish weight range used in this study, 100-200 mg/kg vitamin C (APP) can be considered as the optimum level for rearing the fish.
    Keywords: Aquaculture ; Growth rate ; Vitamin C ; Feeding ; Juveniles ; Condition factor ; Huso huso ; Brackish ; Caspian Sea ; Iran
    Repository Name: AquaDocs
    Type: article , TRUE
    Format: application/pdf
    Format: application/pdf
    Format: 107-120
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  • 4
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25651 | 18721 | 2018-10-14 02:48:20 | 25651 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: This study was conducted to codify the guideline and indexes of broodstock management in salmonid fish and cryopreservation, selective breeding and sex reversal in hatchery to attain responsible aquaculture development. Cryopreservation includes of sperm and egg in term of economic is important in aquaculture. In this report mentioned the type and name of material. All female and sex reversal fish are produced by steroid hormone which mentioned in this report. Selective breeding include individual selection and family selection which could be used for genetic improvement of fish.
    Keywords: Aquaculture ; Biology ; Management ; Iran ; Broodstock ; Reproduction ; Cryopreservation ; Genetic ; Breeding ; Aquaculture ; Salmonid fish ; Sex reversal ; Hatchery ; Sperm ; Egg ; Female ; Steroid hormones
    Repository Name: AquaDocs
    Type: monograph
    Format: application/pdf
    Format: application/pdf
    Format: 46
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  • 5
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    In:  http://aquaticcommons.org/id/eprint/22103 | 18721 | 2018-02-08 03:27:52 | 22103 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-06-26
    Description: A feeding trial was conducted to examine the effects of dietary supplementation of lysine and methionine on growth, nutrient utilization and carcass compositions in beluga, Huso huso. Four experimental soy protein based diets D0 (without lysine or methionine supplementation), D1 (lysine supplementation alone), D2 (methionine supplementation alone) and D3 (both lysine and methionine supplementation) were fed to triplicate groups. 240 fish with an average weight of 144.6 ± 4.64 g were fed the experimental diets for 12wk. L-Lysine and DL-methionine were added to the diets containing 344 g kg-1 soybean meals at 22.02 and 12.94 g kg-1 of dry diet respectively. Significant higher weight gain, specific growth rate, protein efficiency ratio, condition factor, feed efficiency ratio, whole muscle protein and protein apparent digestibility were observed in fish fed soya protein-based diet supplemented with both lysine and methionine than that of fish of other dietary groups. There were no significant differences among fish fed diet supplemented with either methionine or lysine. Growth performances, feed utilization, carcass composition and apparent digestibility coefficients of protein, lipid and dry matter of fish fed control diet was significantly lower than those of fish fed diet supplemented both lysine and methionine and supplemented with either methionine or lysine. It can be concluded that beluga, fingerlings effectively utilize the supplemental amino acids (L-Lysine at 22.02 g kg-1 dry diet and DL-methionine at 12.94 g kg-1 dry diet) in the diets containing 344 g kg-1 soybean meals for better carcass proximate compositions, growth performance and feed efficiency and utilization.
    Keywords: Aquaculture ; Biology ; Chemistry ; Soy protein ; Nutrient utilization ; Apparent digestibility coefficients ; L-Lysine ; DLmethionine ; Beluga ; Huso huso ; diet ; growth ; compositions ; lysine ; Iran
    Repository Name: AquaDocs
    Type: article , TRUE
    Format: application/pdf
    Format: application/pdf
    Format: 119-134
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  • 6
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    In:  http://aquaticcommons.org/id/eprint/22226 | 18721 | 2018-02-24 15:34:50 | 22226 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-06-30
    Description: The study on the ecophysiological impacts of stress in female Persian sturgeon broodfishes revealed that the average cortisol level varried in all broodfish caught at the Sefidrud River (163.38±21.2ng mL-1), Sefidrud River estuary (260.27±19.7ng mL-1) and Gorganrud River estuary (179.38±20.8ng mL-1). The variations in serum glucose levels were 140.65±12.05mg dL-1, 144.44±5.9mg dL-1 and 126.24±11mg dL-1, respectively. The maximum level of cortisol (781 ng mL-1) and serum glucose (281 mg dL-1) were observed during the transport of broodfish from the capture stations to the hatcheries and the minimum level of cortisol (25.2 ng mL-1) and serum glucose (33 mg dL-1) were observed during the confinement of broodfishes in the breeding ponds. The rhythmic and significant variations of cortisol level during capture, handling, transport and confinement of female Persian sturgeon broodfish in all three regions can be regarded as a valuable indicator of stress
    Keywords: Aquaculture ; Biology ; Stress ; Cortisol ; Glucose ; Acipenser persicus ; Broodfish ; Iran
    Repository Name: AquaDocs
    Type: article , TRUE
    Format: application/pdf
    Format: application/pdf
    Format: 37-52
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  • 7
    Publication Date: 2021-06-30
    Description: The effects of stocking density of eggs and larvae were studied related to the morphological deformities appearance, percentage of survival, and growth rate, in order to achieve the most suitable stocking density for eggs and larvae in incubators and rearing tanks. The egg stocking densities were 500, 700 and 900 g per incubator for great sturgeon, 500 and 1000g per incubator for Persian sturgeon and 400 and 425g per incubator for stellate sturgeon. Larvae were stocked at densities of 10000, 15000 and 20000 larvae per m3 for great sturgeon, 21660, 41004 and 69000 larvae per m3 for Persian sturgeon and 15770, 23250, 57660 and 62250 larvae per m3for stellate sturgeon. The results indicate significant differences in percentage of survived in fertilized eggs (p〈0.05). By transferring the larvae into the fibreglass tanks, growth and survival rate decreased, whereas mortality and incidence of deformities increased as stocking density increased particularly on the onset of exogenous feeding. In the present study totally 20 types of deformities including bent spine, hunched back, hanging or looped tail, fin deformation, stunted body length and etc. were registered. The results showed that the morphological deformities frequency in dead and live fish were higher in high stocking densities comparing to the low stocking densities.
    Keywords: Aquaculture ; Biology ; Great sturgeon ; Persian sturgeon ; stellate sturgeon ; Morphological deformities ; Stocking density ; Larval survival ; Iran
    Repository Name: AquaDocs
    Type: article , TRUE
    Format: application/pdf
    Format: application/pdf
    Format: 75-90
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  • 8
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    In:  http://aquaticcommons.org/id/eprint/24686 | 18721 | 2018-08-04 15:58:43 | 24686 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-15
    Description: A two-phase random growth test was conducted to evaluate the effects of feeding rate on growth performance, food conversion ratio (FCR), specific growth rate (SGR), feeding efficiency and price index of reared Huso huso fingerlings in fiberglass tanks (2x2x0.53 cm). Rearing conditions such as diet, dissolved oxygen, light, water flow, fish density and feeding frequency were kept similar for the tanks. In the first phase, 180 fingerlings with a mean weight 867.86±17.42g were fed for 100 days in four treatments and three replications at feeding rates 1, 2, 3 and 4 percent of their biomass. In the second phase, also four treatments and three replications were applied to 84 Huso huso with a mean weight 2096.1±35.6g. In this phase, the fish were fed for 125 days with 0.75, I .5, 2.5 and 3 percent of their biomass. During rearing period, water temperature was 25.52±1.78 and 14.82±0.48 degrees centigrade and dissolved oxygen was 7.6±0.3 and 7.89±0.18 mg/lit, for the two phases respectively. The fishes were fed four times daily with a diet containing 40% protein, 13% fat and 9.9% ash. All treatments induced fast growth in the fish, but feeding ratios were different in their effects on the growth (P〈0.05). Weight gain percentage, specific growth rate (SGR), food conversion ratio (FCR), feeding efficiency and price index indicated that lower feeding ratios were more effective in causing fish growth (P〈0.05). So, increase in feeding ratio directly increased daily food consumption (D.F.C) and negatively affected the feeding efficiency, food conversion ratio, specific growth rate and price index (P〈0.05). The results showed that in phase one, when the fish were given food as much as two percent of their body weight, one unit of meat was produced by consuming 1.92 units of food. In the second phase, feeding fish with 0.75 percent of their body weight resulted in producing one unit of fish meat per 1.82 units of food consumed. Also, in the two phases, application of higher than 50% feeding ratio increased costs while no significant trend in fish growth was detected. It can be concluded that regarding the water temperature, the optimal feeding rate for fishes weighing 850 to 1900 grams and those weighing 2050 to 3300 grams are 2 % and 1 % of body weight, respectively.
    Keywords: Aquaculture ; Huso huso ; Food Conversion Ratio ; Growth Trend ; Iran
    Repository Name: AquaDocs
    Type: article , TRUE
    Format: application/pdf
    Format: application/pdf
    Format: 165-180
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  • 9
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    In:  http://aquaticcommons.org/id/eprint/21932 | 18721 | 2018-01-17 12:21:51 | 21932 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-05
    Description: Short term preservation of spermatozoa of salmonid fishes for practical use, increasing of reproduction potentials and reducing of expenses, has an important role in hatcheries. In this study spermatozoan motility and fertilization rate of 29 commercially cultured rainbow trout were examined. Semen was collected by abdominal stripping at the volume of 4.49±1 (mean±SD) ml and sperm concentration was determined by heamocytometer following 1:600 dilution with saline solution (0.9% NaCl). To find the effects of preservation time on fertilization capacity, samples were diluted with extender (Glucose 54gL-1, Tris 0.5gL-1, DMSO 9% , Egg yolk 20%) and held at 3 different cool conditions : room temperature (+15°C), refrigerator (+5°C) and crashed ice (+ 1-2°C) and sperm motility followed up each 30 mins until complete ceasation. Fertilization, hatching and larval stage rate were determined after 24, 48 and 72 hours. Results showed that the motility of sperm samples held at 15 °C and 5°C were completely ceased after 2 and 24 hours respectively. However it lasted up to 144 hours at1-2°C.Fertilization, hatching and larval stage rates of sperm samples hold at 1- 2°C were 68.45% , 62.1% and 58.2% (after 24 hours), 53.2% , 43.2% and 40.9% (after 48 hours) and 36.6% , 31.6% and 23.7% after 72 hours, respectively, and all showed a significant decrease compared with control (90.32%, 83.27% and 81.12% respectively). Comparing the results showed that holding samples in test tubes at 1-2°C is the best method for short term storage of rainbow trout spermatozoa.
    Keywords: Aquaculture ; Biology ; Spermatozoa ; Short term preservation ; Oncorhynchus mykiss ; Motility rate ; Fertilization ; Iran rate
    Repository Name: AquaDocs
    Type: article , TRUE
    Format: application/pdf
    Format: application/pdf
    Format: 157-164
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  • 10
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    In:  http://aquaticcommons.org/id/eprint/22130 | 18721 | 2018-02-12 11:25:01 | 22130 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-06-27
    Description: In this study the rainbow trout (Oncorhynchus mykiss) myostatin gene (MSTN) for detecting gene derived SNPs and performing genetic association study between MSTN polymorphisms and growth traits were analyzed. Twenty broodstocks of rainbow trout in Genetic and Breeding for coldwater Fishes center in Yasouj for generating full sibling were selected and tagged and then juveniles were raised at the ponds until 5 months post-hatching. DNA extract based on standard method and these individuals selected randomly for molecular analysis. Two single nucleotide polymorphisms (SNPs: g.660T〉C and g.1904C〉A) in intron 1 and 2 regions of the MSTN gene in rainbow trout by were identified DNA sequencing and polymerase chain reaction single-stranded conformational polymorphism (PCR-SSCP) methods. The assessment results demonstrated that 6 haplotypes were revealed in this center. Moreover, association analysis indicated that the genotypes of SNP g.1904C〉A are associated with the length and body weight at 90 and 150 days of age (p〈 0.05), whereas no significant association was found between the SNP g.660T〉C and growth traits. Our results provided evidence that SNPs in MSTN gene may be associated with length and body weight at fingerling age and MSTN gene may be used as potential candidate gene for marker-assisted selection (MAS) in rainbow trout breeding program.
    Keywords: Aquaculture ; Biology ; Oncorhynchus mykiss ; MSTN gene (MSTN) ; Quantitative traits ; Single nucleotide polymorphism (SNP) ; Marker assisted selection ; rainbow trout ; polymorphisms ; weight ; growth ; length ; Iran
    Repository Name: AquaDocs
    Type: article , TRUE
    Format: application/pdf
    Format: application/pdf
    Format: 25-40
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