ALBERT

Description: Despite international commitments to integrate indigenous peoples and their Traditional Ecological Knowledge (TEK) in actions combating climate change, their inclusion remains limited. Integrating TEK with scientific knowledge has become particularly important in sectors such as agriculture, which both contributes to and is affected by climate change. While there is a general recognition that integrating TEK will contribute to climate change adaptation, agricultural interventions have made little progress in achieving this due to the assumption of a clear divide between TEK and scientific knowledge. This paper considers that knowledge integration is already occurring, but in contexts of economic, sociocultural, and political inequalities. We elaborate on the case of traditional weather forecasting methods used by Mayan indigenous farmers in Mexico's Yucatán Peninsula to propose a social justice perspective for knowledge integration in climate change interventions. Using information from three studies conducted between 2016 and 2019, we first explain the importance of weather and traditional weather forecast methods for indigenous Mayan farmers. Later we describe in detail both these methods and their links with Mayan cosmology. Findings show how weather phenomena such as drought and hurricanes are main concerns for milpa farming. They illustrate the diversity of traditional short, medium, and long-term weather forecast methods based on observations from nature and the sky. Farmers also perform rituals that are related to their Mayan gods and goddess. As TEK not only defines agricultural calendars but also reproduces Mayan culture, we discuss what is needed for its integration into actions combating climate change. We use a rights-based approach that considers the economic, cultural, and political scales of justice to equally allocate resources and benefits for traditional knowledge systems, recognize indigenous values and worldviews avoiding cultural harms, and accomplish indigenous self-determination through equal representation. As a result, we hope to incentivize development actors engaged in agricultural interventions on climate change to critically reflect and examine power dynamics and relations when working with indigenous communities.

Description: Cyclin M (CNNM1-4) proteins maintain cellular and body magnesium (Mg2+) homeostasis. Using various biochemical approaches, we have identified members of the CNNM family as direct interacting partners of ADP-ribosylation factor-like GTPase 15 (ARL15), a small GTP-binding protein. ARL15 interacts with CNNMs at their carboxyl-terminal conserved cystathionine-β-synthase (CBS) domains. In silico modeling of the interaction between CNNM2 and ARL15 supports that the small GTPase specifically binds the CBS1 and CNBH domains. Immunocytochemical experiments demonstrate that CNNM2 and ARL15 co-localize in the kidney, with both proteins showing subcellular localization in the endoplasmic reticulum, Golgi apparatus and the plasma membrane. Most importantly, we found that ARL15 is required for forming complex N-glycosylation of CNNMs. Overexpression of ARL15 promotes complex N-glycosylation of CNNM3. Mg2+ uptake experiments with a stable isotope demonstrate that there is a significant increase of 25Mg2+ uptake upon knockdown of ARL15 in multiple kidney cancer cell lines. Altogether, our results establish ARL15 as a novel negative regulator of Mg2+ transport by promoting the complex N-glycosylation of CNNMs.