ISSN:
1432-072X
Keywords:
Bacteriochlorophyll a
;
Associated polypeptides
;
Rhodopseudomonas capsulata
;
Membrane differentiation
;
Photosynthetic apparatus
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Early stages in the formation of membranes and photosynthetic units were studied under growth-limiting phototrophic and chemotrophic conditions in cells of Rhodopseudomonas capsulata. The incorporation of polypeptides, forming bacteriochlorophyll-carotinoid-protein complexes in the membrane, was followed by use of pulse-labeling and immunoprecipitation techniques. The newly synthesized polypeptides were inserted into two distinct membrane fractions at both different rates and proportions. The two membrane fractions differed in sedimentation behavior, absorption spectra and activities of the respiratory chain. The individual pigment-associated proteins did not exhibit precursor-product relationship between the two membrane fractions. The data suggest that newly synthesized polypeptides were integrated both into cytoplasmic and pre-existing intracytoplasmic membranes, where the proteins and pigments were assembled to form reaction centers and light-harvesting pigment-protein complexes.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00405916
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