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  • Associated polypeptides  (1)
  • Bacteriochlorophyll, emission, absorption  (1)
  • 2020-2022
  • 1980-1984  (2)
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Erscheinungszeitraum
  • 2020-2022
  • 1980-1984  (2)
Jahr
  • 1
    Digitale Medien
    Digitale Medien
    Springer
    Archives of microbiology 128 (1981), S. 376-383 
    ISSN: 1432-072X
    Schlagwort(e): Bacteriochlorophyll a ; Associated polypeptides ; Rhodopseudomonas capsulata ; Membrane differentiation ; Photosynthetic apparatus
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Early stages in the formation of membranes and photosynthetic units were studied under growth-limiting phototrophic and chemotrophic conditions in cells of Rhodopseudomonas capsulata. The incorporation of polypeptides, forming bacteriochlorophyll-carotinoid-protein complexes in the membrane, was followed by use of pulse-labeling and immunoprecipitation techniques. The newly synthesized polypeptides were inserted into two distinct membrane fractions at both different rates and proportions. The two membrane fractions differed in sedimentation behavior, absorption spectra and activities of the respiratory chain. The individual pigment-associated proteins did not exhibit precursor-product relationship between the two membrane fractions. The data suggest that newly synthesized polypeptides were integrated both into cytoplasmic and pre-existing intracytoplasmic membranes, where the proteins and pigments were assembled to form reaction centers and light-harvesting pigment-protein complexes.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 2
    ISSN: 1432-072X
    Schlagwort(e): Membrane differentiation ; Buoyant density ; Photosynthetic units ; Bacteriochlorophyll, emission, absorption ; Phospholipids
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Aerobically in the dark grown cultures of Rhodopseudomonas capsulata were shifted to low oxygen partial pressure for 30 min and afterwards to phototrophic conditions (anaerobic, light). During 210 min of adaptation to a phototrophic mode of life the bacteriochlorophyll (BChl) concentration increased 53-fold (doubling time 40 min) and the carotenoid content six fold. Growth was delayed. The light membrane fraction from chemotrophic and induced phototrophic cells contained low concentrations of small photosynthetic units (reaction center+light harvesting BChl B870), and low respiratory activities, especially of succinatecytochrome c oxidase. The heavy membrane fraction, i.e. the intracytoplasmic chromatophore fraction, increased during adaptation approximately 9-fold in surface area per cell, 42-fold in BChl content, 7-fold in reaction center content and 6-fold in the size of the photosynthetic unit. Phospholipid and fatty acid content and patterns changed slightly during adaptation.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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