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  • 1
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Molecular microbiology 1 (1987), S. 0 
    ISSN: 1365-2958
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: The nucleotide sequence of the virG locus of the nopaline type plasmid pTiC58 of Agrobacterium tumefaciens has been determined. It contains an open reading frame (ORF) of 759 nucleotides and has 77% homology to the virG sequences of octopine type plasmids. Differences between the sequences of the two types of Ti plasmids in the region of virG are located predominantly outside the ORF. The amino acid sequences inferred from the two virG genes show 80% homology to each other and each shows the same moderate homologies to amino acid sequences derived from genes in a family of two-component regulatory systems. Specific differences in nucleotide and amino acid sequences as well as a structure-function model for the gene product are discussed.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1095-8649
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Recently hatched fry of brown trout were exposed to each of eight trace metals (Al, Cd, Cu, Fe, Mn, Ni, Pb, Zn) and to a mixture of all eight, at concentrations typical of soft acid waters, in flowing artificial soft water medium at pH 4·5 and 6·5, for 30 days. At pH 4·5, in the absence of trace metals, net uptake of Ca, Na and K and skeletal calcification were impaired but mortalities were low (10%). At pH 4·5, in the presence of Al, Cu, Pb and Zn individually and the mixture, mortalities were high (87–100%). Cd, Fe, Mn and Ni at pH 4·5 each caused some mortalities (17–43%) and impaired skeletal calcification. At pH 6·5, each of Al, Cd, Cu, Fe, Mn, Pb and the mixture of metals impaired net Ca uptake and (except Al, Fe and Pb) skeletal calcification. Net K uptake was impaired by Al and by the mixture, and less severely by Cu and by Fe. Net Na uptake was impaired by Al and by the mixture, and less severely by Fe and by Mn. The role of trace metals other than Al in fisheries’decline in soft acid waters is discussed.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of fish biology 35 (1989), S. 0 
    ISSN: 1095-8649
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Yolk-sac fry of brown trout were exposed to three levels of single trace metals (Cu, 20,40,80 nmol 1-1; Pb, 12·5,25,50 nmol 1-1; Zn, 75,150,300 nmol 1-1) typical of concentrations reported for acid soft waters, in flowing, artificial, soft water media maintained at pH 4·5 and [Ca] of 20 or 200 μmol 1-1for 30 days.Mortalities were high in fry subjected to all levels of the three trace metals at [Ca] 20 μmol 1-1, with 80% of the total deaths occurring between days 11 and 15 of the experiment. 25% mortality occurred at low [Ca] and pH 4·5 in the absence of trace metals, with only one death recorded at [Ca] 200 μmol1-1’(Cu, 80 nmol 1-1). At high [Ca] all three levels of Cu and Pb impaired net Na and K uptake; Cu was the only metal to reduce the uptake of Ca. The Zn treatments had no significant effect on mineral uptake. Calcification of centra was reduced by all three Cu treatments at [Ca] 200 μmol 1-1. The lowest Zn concentration (75 nmol 1-1) was the only other treatment to impair skeletal development. In the absence of trace metals, low [Ca] significantly reduced Ca, Na and K uptake, skeletal calcification and dry mass at pH 4·5.The deleterious effects of low Cu, Pb and Zn concentrations at low pH and low [Ca], and the ameliorative effect of higher ambient [Ca], are discussed in relation to fishery status in soft, acid waters.
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  • 4
    ISSN: 1095-8649
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: In 30-day exposures in artificial soft water medium, survival of brown trout alevins was not affected by low pH (4.5,4.8, 5.4), by low calcium concentration (10.25 μmol l−1) or by manganese (≤20 μmol l−1), but was impaired by aluminium (6–8 μmol l−1) at low calcium concentration (10 μmol l−1) irrespective of pH (4.5 or 5.4). Manganese (6.6, 20 μmol l−1) impaired net calcium uptake and calcium deposition in the skeleton at low calcium concentration (25 μmol l−1) irrespective of pH. Aluminium (2–8 μmol l−1) impaired gross development, net uptake of calcium, potassium and sodium, and calcium deposition in the skeleton, and slightly increased the net loss of magnesium, some of these effects being more severe at calcium concentration 10 μmol l−1 than 50 μmol l−1, and some more severe at pH 5.4 than pH 4.5. Net uptake of calcium and sodium were impaired at low pH (4.5, 4.8), and skeletal calcium deposition was impaired at low calcium concentration (10 μmol l−1), but these effects of low pH and low calcium concentration were slight compared with those of the trace metals. The possible role of trace metals in reports of the deleterious effects on fish of low pH levels is discussed.
    Type of Medium: Electronic Resource
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