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  • Springer  (56)
  • 2020-2022  (41)
  • 1985-1989  (15)
  • 1960-1964
  • 1
    Electronic Resource
    Electronic Resource
    Use of BRL-conditioned medium in combination with feeder layers to isolate a diploid embryonal stem cell line (1989)
    Springer
    Development genes and evolution 198 (1989), S. 48-56 
    Details
    ISSN: 1432-041X
    Keywords: Embryonal stem cells ; Conditioned medium ; Feeder layer
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The derivation of a karyotypically normal embryonal stem (ES) cell line, E14, from inner cell masses (ICMs) isolated by immunosurgery from 129/Ola late mouse blastocysts is described. Disaggregated ICMs were cultured on mitotically-arrested fibroblast feeder layers in droplets of medium conditioned with Buffalo rat liver (BRL) cells under oil. BRL-conditioned medium inhibits the differentiation of established embryonal carcinoma (EC) and ES cell lines which can be maintained indefinitely in the complete absence of feeder cells (Smith and Hooper 1987). At clonal densities, however, a combination of BRL-conditioned medium and a feeder layer was most effective in preventing the differentiation of E14 cells. This effect was less pronounced at higher passage suggesting it may be particularly important to use a combination in the early stages of isolation. Once established, E14 has been maintained in BRL-conditioned medium alone. In non-conditioned medium on agarose, E14 cells formed embryoid bodies which when allowed to reattach differentiated into a wide variety of tissues. An HPRT-deficient sub line of E14, E14TG2a, has been demonstrated to form germline chimaeras with high efficiency after injection into blastocysts (Hooper et al. 1987). The modifications to the ES cell isolation procedure described here may improve the efficiency with which karyotypically normal lines can be derived.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00376370
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  • 2
    Electronic Resource
    Electronic Resource
    Protein phosphorylation and compartments of cyclic AMP in the control of cardiac contraction (1989)
    Springer
    Molecular and cellular biochemistry 89 (1989), S. 175-179 
    Details
    ISSN: 1573-4919
    Keywords: cyclic AMP ; protein phosphorylation ; cardiac contraction ; compartmentation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract The roles of cyclic AMP, cyclic AMP-dependent protein kinase and the phosphorylation of specific proteins in the regulation of cardiac contractility are briefly reviewed. Criteria for determining whether changes in cyclic AMP and protein phosphorylation are involved in a physiological response are discussed. Although cyclic AMP-dependent phosphorylation of the voltage-operated Ca2− channel, phospholamban, troponin-I and C-protein have all been implicated in the response of the heart to inotropic agents which elevate cyclic AMP, none of these phosphorylations satisfy all of the criteria completely. Evidence is presented that there are compartments of cyclic AMP in heart which are coupled to different functional responses.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00220772
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  • 3
    Electronic Resource
    Electronic Resource
    Estimating the sensitivity of natural ecosystems and agriculture to climatic change — Guest editorial (1985)
    Springer
    Climatic change 7 (1985), S. 1-3 
    Details
    ISSN: 1573-1480
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences , Physics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00139437
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  • 4
    Electronic Resource
    Electronic Resource
    Extra-pair fertilizations in mountain white-crowned sparrows (1988)
    Springer
    Behavioral ecology and sociobiology 22 (1988), S. 413-420 
    Details
    ISSN: 1432-0762
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A population of white-crowned sparrows (Zonotrichia leucophrys oriantha) located near Tioga Pass, in the central Sierra Nevada of California, was studied during the 1985 and 1986 breeding seasons to determine how often chicks were not the offspring of both adults who reared them. Blood samples were obtained from 35 nesting pairs and feather pulp specimens were collected from all 110 young they fledged. Electrophoretic analyses of 4 polymorphic loci revealed that a minimum of 9 nests (26%) contained a chick that could not have been conceived in a mating between the attending pair, and that at least 15 chicks (14%) were not offspring of both attending adults. Using two recently developed probability indices, it was estimated that 34%–38% of chicks hatched at the study site were not offspring of one of their putative parents. Because there was no evidence that intraspecific brood parasitism occurred, all the mismatched young apparently resulted from extrapair copulations. Our data, along with similar results from other species, raise new questions about sexual selection and patterns of parental care in monogamous birds.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00294979
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  • 5
    Electronic Resource
    Electronic Resource
    Lowered induction of genetic tandem duplications in Salmonella by the pKM101 plasmid (1986)
    Springer
    Molecular genetics and genomics 204 (1986), S. 281-284 
    Details
    ISSN: 1617-4623
    Keywords: Error-prone repair ; Genetic duplication ; Carcinogens ; Mutagens ; Recombination
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Selection for 3-amino-1,2,4-triazole (AT) resistance in certain strains of Salmonella typhimurium has been previously shown to select for genetic tandem duplications of the histidine operon. We show here that agents which induce tandem duplications are less effective in such induction in the presence of the pKM101 plasmid. The presence of the plasmid also produces an increase in AT-resistance due to mechanisms other than duplication, presumably because pKM101 produces high levels of error-prone repair. We suggest that high levels of error-prone repair may cause decreases in tandem duplication induction and propose that error-prone repair and tandem duplication may be alternative cellular responses to certain DNA lesions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00425510
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  • 6
    Electronic Resource
    Electronic Resource
    Distribution and morphology of columnar cacti in tropical deciduous woodland, Jalisco, Mexico (1986)
    Springer
    Plant ecology 66 (1986), S. 137-145 
    Details
    ISSN: 1573-5052
    Keywords: Branching pattern ; Cactus ; Light regime ; Mexico ; Morphology ; Vegetation height
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The distribution and branching morphologies of four species of columnar cactus species in tropical deciduous woodland in Jalisco, western Mexico, are examined. Two species, Stenocereus marginatus and especially Pachycereus pecten-aboriginum, are taller and extend through the woodland canopy, and only these two species occupy habitats where the woodland vegetation is taller, on N- and NE-facing aspects where incident radiation levels are lowest. Two species, Stenocereus standleyi and Cephalocereus purpusii, are shorter, subcanopy species, and are more restricted to low-angle slopes and slopes with S- to W-aspects, where the woodland vegetation is shorter and more open, with a greater penetration of radiation into the vegetation. Branching morphology is affected by the vegetation cover in all species, but in different ways between the two taller and the two shorter species. Ecological segregation in these four species is achieved apparently by differences among the light regimes of subhabitats correlated to the morphology of the cacti. This contrasts to segregation in Sonoran Desert columnar cactus species, which segregate by different strategies of water uptake and utilization, and in columnar cacti in woodlands in Oaxaca further south in Mexico, where adult individuals are very similar in branching morphology, but the younger individuals differ in this respect and presumably become established in different sorts of light gaps.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00039907
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  • 7
    Electronic Resource
    Electronic Resource
    Replication of pSV2-gpt in COS-1 cells: Stability of plasmid DNA in the presence and absence of biochemical selection (1985)
    Springer
    Somatic cell and molecular genetics 11 (1985), S. 167-176 
    Details
    ISSN: 1572-9931
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract We have previously demonstrated that COS-1 cell lines transformed by pSV2-gpt and maintained under biochemical selection replicate multiple copies of extrachromosomal plasmid DNA (1). We have now examined the replication and stability of this DNA in a representative cell line. In situ hybridization analyses revealed that intense replication of pSV2-gpt occurs in only a small subpopulation of cells and results from bursts of plasmid replication that occur periodically and spontaneously in the cell population. This suggests that COS-1 cells are only semipermissive for pSV2-gpt replication. No correlation was observed between levels of pSV2-gpt replication and the presence or absence of biochemical selection for the Gptmarker. However, growth of cells under nonselective conditions led to a rapid and progressive loss of pSV2-gpt DNA. This loss correlated with segregation of Gpt − revertants that lacked detectable plasmid sequences. Hence, maintenance of pSV2-gpt in the cell line was dependent on continuous biochemical selection. Stable replication of pSV2-gpt could be observed as late as four months after transfection, suggesting that this system might be useful for propagation of cloned DNA in COS-1 cells for extended periods of time. However, by nine months, extensive rearrangements of pSV2-gpt sequences were detected, indicating ultimate instability of the plasmid in the host cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01534705
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  • 8
    Electronic Resource
    Electronic Resource
    Carnoya kermarreci n.sp. (Rhigonematidae: Nematoda) from Anadenobolus politus (Porat) (Rhinocricidae: Diplopoda) from Guadeloupe (1985)
    Springer
    Systematic parasitology 7 (1985), S. 27-32 
    Details
    ISSN: 1573-5192
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Carnoya kermarreci n.sp. (Rhigonematidae; Nematoda) is described from Anadenobolus politus (Porat) (Rhinocricidae; Diplopoda) from Guadeloupe. The new species most closely resembles C. martiniquensis Adamson, 1984 from A. politus in Martinique and C. venezuelensis Adamson, 1984 from Rhinocricus flavocinctus from Venezuela in that the lips in the male are broad and the arrangement of caudal papillae is almost identical in the three species. Males of C. kermarreci are easily distinguished by the absence of spines, by the form of the cuticular projections at the base of the buccal cavity and by the fact that the most anterior pair of caudal papillae are located very close together. Females are distinguished by the presence of three pairs of somatic papillae near the level of the vulva. The nematode fauna of A. politus includes species from two distinct orders and presumably reflects the fact that diplopods are among the most ancient hosts of nematodes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00010158
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  • 9
    Electronic Resource
    Electronic Resource
    Fatty acid control of cyclic AMP levels inNeurospora crassa (1987)
    Springer
    Current microbiology 15 (1987), S. 25-27 
    Details
    ISSN: 1432-0991
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Several saturated, monosaturated, and polyunsaturated fatty acids produce rapid increases in cyclic AMP levels in the fungusNeurospora crassa when added to the growth medium at 10–50 μM. The time courses of cyclic AMP increase resembled those previously shown to be induced by other agents, reaching peak cyclic AMP levels at about 2 min after fatty acid addition. These fatty acids had little or no influence on adenylate cyclase fromNeurospora crassa in vitro. On the basis of previous evidence that uncouplers of oxidative phosphorylation increase cyclic AMP levels and that fatty acids can act as uncouplers, we suggest that the fatty acids in vivo may act to increase cyclic AMP levels by acting as uncouplers of oxidative phosphorylation. In agreement with this suggestion, two fatty acids were shown to produce decreased ATP-ADP ratios inNeurospora at concentrations producing cyclic AMP increases.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01577209
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  • 10
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    Erratum to: Updated measurement of time-dependent $$C!P$$-violating observables in $${{B} ^0_{s}} ightarrow J/psi K^+ K^-$$ decays (2020)
    Springer
    Details
    Publication Date: 2020-07-01
    Description: In the Introduction section of the original article [1].
    Print ISSN: 1434-6044
    Electronic ISSN: 1434-6052
    Topics: Physics
    Published by Springer
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