ISSN:
1432-072X
Keywords:
Denitrification
;
N2O reductase
;
Nitrite reductase
;
Immunocytochemistry
;
Localization
;
Two-dimensional electrophoresis
;
Cell fractionation
;
Pseudomonas stutzeri
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Immunogold labelling techniques on ultrathin sections of low temperature embedded cells yielded evidence for the periplasmic location of the respiratory enzymes N2O reductase and nitrite reductase (cytochrome cd 1) in Pseudomonas stutzeri strain ZoBell. Cell fractionation by spheroplast preparation and two-dimensional electrophoresis showed the absence of a membrane association of these enzymes. Immunocytochemical localization of N2O reductase in a mutant strain deficient in the chromophore of N2O reductase showed the gold label at the cell periphery, indicating that the copper chromophore processing takes place after export of this protein's apoform.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00245153
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