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  • Articles  (35)
  • Springer  (17)
  • Wiley  (11)
  • American Meteorological Society  (3)
  • Blackwell Publishing Ltd  (2)
  • Cell Press  (2)
  • Heidelberg: Springer
  • 2020-2022  (9)
  • 1990-1994  (14)
  • 1980-1984  (12)
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  • Articles  (35)
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  • 1
  • 2
    Publication Date: 2021-05-25
    Print ISSN: 0012-9615
    Electronic ISSN: 1557-7015
    Topics: Biology
    Published by Wiley on behalf of Ecological Society of America.
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  • 3
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Annals of the New York Academy of Sciences 675 (1992), S. 0 
    ISSN: 1749-6632
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Natural Sciences in General
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 90 (1994), S. 0 
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The specific activities of 6 enzymes, which are involved in the synthesis and catabolism of membrane lipids, were monitored in plasma membranes isolated from petunia petals during senescence. These included phosphatidylinositol (PI) kinase (EC 2.7.1.67), phosphatidylinositol monophosphate (PIP) kinase (EC 2.7.1.68). diacylglycerol (DAG) kinase (EC 2.7.1.107), phospholipase A (EC 3.1.1.4) and PIP- and PIP2-phospholipase C˙(EC 3.1.4.3). Using endogenous substrate, the [32P]PA and [32P]PIP2 formation increased to 140 and 200%, respectively, of the day 1 value by 4 days after harvest. There was no significant change in [32P]PIP formation during the same time period. On the fifth day the petals wilted and the [32P]PA and [32P]PIP formation declined significantly. In contrast, the [32P]PIP2 formation remained high in the day 5 petals. When the lipid kinase activities were assayed in the membranes in the presence of exogenous substrate the specific activity of all of the enzymes increased. and the changes in [32P]PA production over the 5-day period were similar to those observed with endogenous substrate. When exogenous PI and PIP were added, however, there was no longer an increase in [32P]PIP2 formation by plasma membranes of day 4 petals and [32P]PIP formation significantly decreased. The relative decrease in PIP and PIP2 formation by day 4 membranes when exogenous substrate was added may have resulted from differences in the lipase activities in the day 1 and day 4 membranes. The plasma membrane A-type phospholipase activity increased throughout the 5 day period, and phospholipase C activity increased two-fold between day 1 and day 4. Such changes in the metabolism of the plasma membrane lipids during flower senescence would affect the ability of the petals to use inositol phospholipid-based signal transduction pathways.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Computers and the humanities 27 (1993), S. 55-71 
    ISSN: 1572-8412
    Source: Springer Online Journal Archives 1860-2000
    Topics: Computer Science , Media Resources and Communication Sciences, Journalism
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Computers and the humanities 27 (1993), S. 261-275 
    ISSN: 1572-8412
    Keywords: conceptual analysis ; knowledge representation ; knowledge engineering ; semantic networks ; text analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Computer Science , Media Resources and Communication Sciences, Journalism
    Notes: Abstract This article addresses the methodological problem of the non-linear representation of philosophical systems in a computerized knowledge base. It is a problem of knowledge representation as defined in the field of artificial intelligence. Instead of a purely theoretical discussion of the issue, we present selected results of a practical experiment which has in itself some theoretical significance. We show how one can represent different philosophies using CODE, a knowledge engineering system developed by artificial intelligence researchers. The hypothesis is that such a computer based representation of philosophical systems can give insight into their conceptual structure. We argue that computer aided text analysis can apply knowledge representation tools and techniques developed in artificial intelligence and we estimate how philosophers as well as knowledge engineers could gain from this cross-fertilization. This paper should be considered as an experiment report on the use of knowledge representation techniques in computer aided text analysis. It is part of a much broader project on the representation of conceptual structures in an expert system. However, we intentionally avoided technical issues related to either Computer Science or History of Philosophy to focus on the benefit to enhance traditional humanistic studies with tools and methods developed in AI on the one hand and the need to develop more appropriate tools on the other.
    Type of Medium: Electronic Resource
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  • 7
    ISSN: 1432-0983
    Keywords: Transportable elements ; Insertion mutations ; Expression mutants ; Yeast CYC7 gene
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The plasmid YCpCYC7(2) was constructed containing the Saccharomyces cerevisiae CYC7 gene, encoding the iso-2-cytochrome c protein, replicative sequences and selective markers from both E. coli and yeast, and the centromere of yeast chromosome III. The expression of the plasmid-CYC7 gene in yeast was similar to the low level expression characteristic of the chromosomal CYC7 gene. A number of insertions into the sequences 5′ to the gene were constructed in vitro. The insertion at 142 by 5′ to the coding sequence of a 400 by fragment which lies 5′ to the CYC1 gene and is known to be essential for the high rates of CYC1 transcription increased transcription of the CYC7 gene to levels characteristic of CYC1 transcription. On the other hand, the insertion of random DNA fragments at the same position gave mostly decreased CYC7 transcription. In addition to these in vitro constructions, a mutant plasmid was selected which had increased CYC7 transcription. This mutation was caused by the insertion of the bacterial IS1 element 313 by 5′ to the CYC7 coding sequence. The significance of these results is discussed in terms of two alternative models for CYC7 gene expression.
    Type of Medium: Electronic Resource
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  • 8
    ISSN: 1615-6102
    Keywords: Golgi apparatus ; Endoplasmic reticulum ; Polyribosomes ; Golgi apparatus-associated polyribosomes ; Wild carrot (Daucus carota)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Endoplasmic reticulum-polyribosome-Golgi apparatus associations were a general feature of cells of suspension cultures of wild carrot (Daucus carota L.). Free polyribosomes occurred within the Golgi apparatus zone for all dictyosomes and with equal frequency at all levels within the stack including the most mature or trans face. When evaluated and quantified from electron micrographs, approximately 60% of the dictyosome profiles were characterized by a system of transition elements consisting of part smooth-part rough endoplasmic reticulum. These were encountered most frequently in the immediate vicinity of the immature, forming or cis face, usually toward the periphery of the stacked cisternae. Analysis of serial sections showed that those dictyosome profiles not exhibiting this characteristic did so primarily because of an unfavorable plane of sectioning. All dictyosomes examined in 5 or more serial sections revealed some type of close association with endoplasmic reticulum. Some of the associations were so close that direct connections between Golgi apparatus and endoplasmic reticulum tubules could not be excluded. Also present, especially at the forming or cis face, were small 600 nm transition vesicles with nap-like surface coats on nearly 90% of the dictyosomes examined. More than 50% exhibited spiny (clathrin-)coated vesicles at the mature or trans face.
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    Springer
    Protoplasma 120 (1984), S. 209-215 
    ISSN: 1615-6102
    Keywords: Fusion ; Calcium ; Protoplast ; Membrane
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Rather than selecting for a chemical fusogen one can select for a fusogenic plant membrane (i.e., one that will fuse readily). Wild carrot suspension culture cells can be grown under conditions which cause the released protoplasts to have a high potential to fuse. Protoplast fusion is enhanced by calcium and inhibited by EGTA. When 10mM calcium (pH6.0) is added, fusion percentages of 60% are common. The mild fusion treatment appears to have no effect on callus regeneration and differentiation.
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  • 10
    Electronic Resource
    Electronic Resource
    Springer
    Protoplasma 123 (1984), S. 213-220 
    ISSN: 1615-6102
    Keywords: Carrot suspension cultures ; Dictyosomes ; Endoplasmic reticulum ; Golgi apparatus ; Membrane biogenesis ; Plasma membrane
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Wild carrot (Daucus carota L.) cells, grown in suspension culture, were labeled with radioactive precursors and fractionated into constituent membranes to be analyzed for specific radioactivity. Results show rapid incorporation of [3H] leucine into endoplasmic reticulum (ER)-, Golgi apparatus-, and plasma membrane/tonoplast-enriched fractions. The time lag between incorporation into ER and its appearance in Golgi apparatus or plasma membrane/tonoplast were less than 5 minutes. With an average time of 3–4 minutes for cisternal formation estimated from studies with monensin, and an average of 5 cisternae per dictyosome (total transit time of 15–20 minutes), it was not possible to account for early incorporation of radioactivity into plasma membranes by passage of proteins from ER to plasma membrane via the Golgi apparatus. To account for the findings, it would appear that at least some proteins were delivered to the plasma membrane via the first membranes that exited (i.e., mature face vesicles) from the Golgi apparatus post-pulse and that some of these proteins had been translated and inserted into membranes at or near the mature face of the Golgi apparatus.
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