ISSN:
1432-2242
Keywords:
Key wordsBrassica
;
Raphanus
;
Interspecific hybrids
;
Genomic in situ hybridization
;
GISH
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Genomic in situ hybridization (GISH) methods were used to detect different genome components within Brassica amphidiploid species and to identify donor chromatin in hybrids between Brassica napus and Raphanus sativus. In Brassica juncea and Brassica carinata the respective diploid donor genomes could be reliably distinguished by GISH, as could all R-genome chromosomes in the intergeneric hybrids. The A- and C-genome components in B. napus could not be clearly distinguished from one another using GISH, confirming the considerable homoeology between these genomes. GISH methods will be extremely beneficial for monitoring chromatin transfer and introgression in interspecific Brassica hybrids.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s001220050699
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