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  • Gonadotrops  (3)
  • Springer  (3)
  • American Institute of Physics (AIP)
  • American Society of Hematology
  • Institute of Physics
  • Wiley-Blackwell
  • 2020-2022
  • 1995-1999  (3)
  • 1975-1979
  • 1960-1964
Sammlung
Verlag/Herausgeber
  • Springer  (3)
  • American Institute of Physics (AIP)
  • American Society of Hematology
  • Institute of Physics
  • Wiley-Blackwell
Erscheinungszeitraum
  • 2020-2022
  • 1995-1999  (3)
  • 1975-1979
  • 1960-1964
Jahr
  • 1
    ISSN: 1432-0878
    Schlagwort(e): Pituitary ; Gonadotrops ; Crinophagy ; Electron microscopy ; Enzyme cytochemistry ; Immunocytochemistry ; Autoradiography ; Catfish, Clarias gariepinus (Teleostei)
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Abstract The possible function of globules and irregular membrane-bound masses in the gonadotropin cells of the pituitary of Clarias gariepinus was studied. Strong secretory stimulation led to the disappearance of the secretory granules from gonadotropin cells but globules and irregular masses remained present. Acid phosphatase was detected enzyme-cytochemically in both globules and irregular masses. Radiolabelling with tritiated amino acids followed by autoradiography demonstrated that globules received radioactive material after secretory granules. The latter received radioactive material within 75 min of administration of radioactive amino acids but globules and irregular masses did not. Although some globules became radioactively labelled within 24 h of the administration of radioactive amino acids, irregular masses remained unlabelled during this period. Secretory granules reacted positively with antisera against α and β gonadotropin subunits, whereas globules and irregular masses only reacted with the antiserum against the β subunit. A moderate anti-7B2 immunoreactivity was demonstrated in secretory granules and globules, whereas irregular masses labelled strongly. The combined cytological results indicate that globules and irregular masses are degradative, possibly crinophagic structures which develop by fusional events from secretory granules to globules and then to irregular masses.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
    BibTip Andere fanden auch interessant ...
  • 2
    ISSN: 1432-0878
    Schlagwort(e): Key words: Pituitary ; Gonadotrops ; Crinophagy ; Electron microscopy ; Enzyme cytochemistry ; Immunocytochemistry ; Autoradiography ; Catfish ; Clarias gariepinus (Teleostei)
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Abstract. The possible function of globules and irregular membrane-bound masses in the gonadotropin cells of the pituitary of Clarias gariepinus was studied. Strong secretory stimulation led to the disappearance of the secretory granules from gonadotropin cells but globules and irregular masses remained present. Acid phosphatase was detected enzyme-cytochemically in both globules and irregular masses. Radiolabelling with tritiated amino acids followed by autoradiography demons trated that globules received radioactive material after secretory granules. The latter received radioactive material within 75 min of administration of radioactive amino acids but globules and irregular masses did not. Although some globules became radioactively labelled within 24 h of the administration of radioactive amino acids, irregular masses remained unlabelled during this period. Secretory granules reacted positively with antisera against α and β gonadotropin subunits, whereas globules and irregular masses only reacted with the antiserum against the β subunit. A moderate anti-7B2 immunoreactivity was demonstrated in secretory granules and globules, whereas irregular masses labelled strongly. The combined cytological results indicate that globules and irregular masses are degradative, possibly crinophagic structures which develop by fusional events from secretory granules to globules and then to irregular masses.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
    BibTip Andere fanden auch interessant ...
  • 3
    ISSN: 1432-0878
    Schlagwort(e): Key words: Pituitary ; Gonadotrops ; Endocytosis ; Crinophagy ; Catfish ; Clarias gariepinus (Teleostei)
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Abstract. The gonadotropin cells of the pituitary of Clarias gariepinus not only contain secretory granules but also globules and irregular masses. These organelles and their relationship with the endocytotic and degradative pathways were investigated. Two endocytotic tracers, horseradish peroxidase (HRP) and wheat-germ agglutinin-conjugated horseradish peroxidase (WGA-HRP), were used to visualise the endocytotic-lysosomal route in the gonadotropin cells. HRP was detected in various types of endocytotic-lysosomal organelles such as endosomes, multivesicular bodies and secondary lysosomes, but not in globules and irregular masses. WGA-HRP was not only found in endosomes, multivesicular bodies and secondary lysosomes, but also in the trans-Golgi network, from where it can enter secretory granules and primary lysosomes. Moreover, 3 h after its administration, WGA-HRP, unlike HRP, was observed in globules and later also in irregular masses. WGA-HRP-labelled secretory granules were often seen closely connected with globules and irregular masses. It was concluded that the label was delivered to the globules and irregular masses by fusion with labelled secretory granules and primary lysosomes. The combined cytological results confirm the idea that globules and irregular masses develop into crinophagic structures.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
    BibTip Andere fanden auch interessant ...
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