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Digitale Medien

Enhancement of Agkistrodon piscivorus piscivorus venom phospholipase A2 activity toward phosphatidylcholine vesicles by lysolecithin and palmitic acid: Studies with fluorescent probes of membrane structure (1995)

Sheffield, Mark J. ; Baker, Brian L. ; Li, Du ; [weitere]

s.l. : American Chemical Society

Biochemistry 34 (1995), S. 7796-7806

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ISSN: 1520-4995

Quelle: ACS Legacy Archives

Thema: Biologie , Chemie und Pharmazie

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1021/bi00024a003

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2

Digitale Medien

On the calculation of sugar concentration in flower nectar (1979)

Bolten, Alan B. ; Feinsinger, Peter ; Baker, Herbert G. ; [weitere]

Springer

Oecologia 41 (1979), S. 301-304

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ISSN: 1432-1939

Quelle: Springer Online Journal Archives 1860-2000

Thema: Biologie

Notizen: Summary There are several sources of potential error in calculating the concentration or energy value of floral nectar. Errors resulting from confusing data become substantial with increasing concentration. The different methods of expressing sugar concentration are here clarified, and the correct methods of converting from one to the other are provided. Refractometers in use in field studies usually read on a weight per total weight basis; this is recommended as the mode of statement. The perils of oversimplifying conversions from this mode, as is often done, are pointed out.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1007/BF00377434

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3

Digitale Medien

Antioxidant survey to assess antagonism to redox stress using a prokaryotic and an eukaryotic system (1996)

Baker, H. ; DeAngelis, B. ; Frank, O. ; [weitere]

Springer

Cellular and molecular life sciences 52 (1996), S. 597-599

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ISSN: 1420-9071

Schlagwort(e): Glutathione ; free radicals ; antioxidants ; oxidant stressors ; buthionine ; menadione

Quelle: Springer Online Journal Archives 1860-2000

Thema: Biologie , Medizin

Notizen: Abstract Using a prokaryote (Escherichia coli) and a metazoa-resembling eukaryote (Ochromonas danica), we surveyed antioxidants which might overcome redox stress imposed by menadione sodium bisulphite (MD) and buthionine sulphoximine (BSO). BSO oxidant stress was evident only inO. danica; MD oxidant stress was evident in both organisms. Glutathione, its precursors, e.g. cysteine, homocysteine, and 2-oxo-4-thiazolidine carboxylic acid, and red blood cells, emerged as prime antioxidants for relieving BSO and MD oxidant stress. BSO and MD oxidant activity and antioxidant-annulling effect inO. danica were judged comparable to those found in animal cells whereas the resultsE. coli were not entirely equivalent. TheO. danica system emerged as a practical, rapid, and useful system for pinpointing oxidant stressors and antioxidants, and shows promise for studies with mammalian systems.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1007/BF01969736

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4

Digitale Medien

Growth hormone receptor expression in the nucleus and cytoplasm of normal and neoplastic cells (1998)

Lincoln, D. T. ; Sinowatz, Fred ; Temmim-Baker, Labiba ; [weitere]

Springer

Histochemistry and cell biology 109 (1998), S. 141-159

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ISSN: 1432-119X

Quelle: Springer Online Journal Archives 1860-2000

Thema: Biologie , Medizin

Notizen: Abstract Growth hormone (GH) exerts its regulatory functions in controlling metabolism, balanced growth and differentiated cell expression by acting on specific receptors which trigger a phosphorylation cascade, resulting in the modulation of numerous signalling pathways dictating gene expression. A panel of five monoclonal antibodies was used in mapping the presence and somatic distribution of the GH receptor by immunohistochemistry in normal and neoplastic tissues and cultured cells of human, rat and rabbit origin. A wide distribution of the receptor was observed in many cell types. Not all cells expressing cytoplasmic GH receptors displayed nuclear immunoreactivity. In general, the relative proportion of positive cells and intensity of staining was higher in neoplastic cells than in normal tissue cells. Immunoreactivity showed subcellular localisation of the GH receptor in cell membranes and was predominantly cytoplasmic, but strong nuclear immunoreaction was also apparent in many instances. Intense immunoreactivity was also observed in the cellular Golgi area of established cell lines and cultured tissue-derived cells in exponential growth phase, indicating cells are capable of GH receptor synthesis. The presence of intracellular GH receptor, previously documented in normal tissues of mostly animal origin, is the result of endoplasmic reticulum and Golgi localisation. Heterogeneity of immunoreactivity was found in normal and neoplastic tissue with a variable range of positive cells. The nuclear localisation of immunoreactivity is the result of nuclear GH receptor/binding protein, identically to the cytosolic and plasma GH-binding protein, using a panel of five monoclonal antibodies against the GH receptor extracellular region. The expression of GH receptors, not only on small proliferating tumour cells such as lymphocytes, but also on well differentiated cells including keratinocytes, suggests that GH is necessary not only for differentiation of progenitor cells, but also for their subsequent clonal expansion, differentiation and maintenance.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1007/s004180050212

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5

Digitale Medien

Crystal structure of Escherichia coli manganese superoxide dismutase at 2.1-Å resolution (1998)

Edwards, Ross A. ; Baker, Heather M. ; Whittaker, M. M. ; [weitere]

Springer

JBIC 3 (1998), S. 161-171

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ISSN: 1432-1327

Schlagwort(e): Key words Superoxide dismutase ; Manganese enzyme ; Crystal structure ; Metalloprotein ; DNA binding

Quelle: Springer Online Journal Archives 1860-2000

Thema: Biologie , Chemie und Pharmazie

Notizen: Abstract The three-dimensional structure of the manganese-dependent superoxide dismutase (MnSOD) from Escherichia coli has been determined by X-ray crystallography at 2.1 Å resolution. The protein crystallizes with two homodimers in the asymmetric unit, and a model comprising 6528 protein atoms (residues 1–205 of all four monomers), four manganese ions and 415 water molecules has been refined to an R factor of 0.188 (R free 0.218). The structure shows a high degree of similarity with other MnSOD and FeSOD enzymes. The Mn centres are 5-coordinate, trigonal bipyramidal, with His26 and a solvent molecule, probably a hydroxide ion, as apical ligands, and His81, Asp167 and His171 as equatorial ligands. The coordinated solvent molecule is linked to a network of hydrogen bonds involving the non-coordinated carboxylate oxygen of Asp167 and a conserved glutamine residue, Gln146. The MnSOD dimer is notable for the way in which the two active sites are interconnected and a "bridge" comprising His171 of one monomer and Glu170 of the other offers a route for inter-site communication. Comparison of E. coli MnSOD and FeSOD (a) reveals some differences in the dimer interface, (b) yields no obvious explanation for their metal specificities, and (c) provides a structural basis for differences in DNA binding, where for MnSOD the groove formed by dimerization is complementary in charge and surface contour to B-DNA.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1007/s007750050217

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