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  • cDNA  (4)
  • Springer  (4)
  • National Academy of Sciences
  • 2020-2022
  • 2005-2009
  • 1990-1994  (4)
  • 1965-1969
  • 1930-1934
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Schlagwörter
Verlag/Herausgeber
  • Springer  (4)
  • National Academy of Sciences
Erscheinungszeitraum
  • 2020-2022
  • 2005-2009
  • 1990-1994  (4)
  • 1965-1969
  • 1930-1934
Jahr
  • 1
    Digitale Medien
    Digitale Medien
    A low-temperature-responsive translation elongation factor 1α from barley (Hordeum vulgare L.) (1993)
    Springer
    Plant molecular biology 23 (1993), S. 221-225 
    Details
    ISSN: 1573-5028
    Schlagwort(e): barley ; cold ; cDNA ; EF-1α ; elongation factor 1α ; low temperature
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract A cDNA clone (pBLT63) encoding a protein synthesis elongation factor 1α (EF-1α) was isolated from a low-temperature winter barley shoot meristem library by differential screening. The nucleotide sequence of the coding region of the low-temperature-induced barley gene shows very high homology with two EF-1α plant genes from tomato and Arabidopsis. The barley genome contains an EF-1α gene family situated on the short arm of chromosome 2 and the long arm of chromosome 5. The nucleotide sequence data reported will appear in the EMBL, GenBank and DDBJ Nucleotide Sequence Databases under the accession number Z23130.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00021434
    Standort Signatur Erwartet Verfügbarkeit
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  • 2
    Digitale Medien
    Digitale Medien
    Molecular analysis and spatial expression pattern of a low-temperature-specific barley gene, blt101 (1993)
    Springer
    Plant molecular biology 23 (1993), S. 871-879 
    Details
    ISSN: 1573-5028
    Schlagwort(e): cold ; low temperature ; barley ; gene expression ; cDNA ; shoot meristem
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract A cDNA clone of the previously unreported low-temperature-induced gene blt101 was isolated after a differential screen of a cDNA library prepared from low-temperature (6 °C day/2 °C night) grown barley shoot meristems. Southern blot analysis of barley ditelosomic addition lines was used to assign this single-copy gene to the long arm of chromosome 4. Analysis of steady-state levels of blt101 mRNA showed the induction of this transcript in shoot meristems upon transfer of barley (cv. Igri) plants from control (20 °C/15 °C) to low (6 °C/2 °C) temperature treatment. Further, the high level of this transcript is maintained at low temperatures but is reduced on transfer from low to control temperatures. The gene is not induced by drought or by foliar application of ABA. Analysis of segregating doubled haploid lines shows that there is no specific association of this gene with either spring/winter growth habit or frost hardiness. Examination of the spatial expression pattern revealed ubiquitous expression of blt101 in low-temperature (6 °C/2 °C) grown barley shoot meristems, mature leaves and roots.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00021541
    Standort Signatur Erwartet Verfügbarkeit
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    Artikel (Nationallizenzen)
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  • 3
    Digitale Medien
    Digitale Medien
    Restriction fragment length polymorphism segregation analysis of the Li locus in Trifolium repens L. (1990)
    Springer
    Plant molecular biology 14 (1990), S. 407-414 
    Details
    ISSN: 1573-5028
    Schlagwort(e): cDNA ; cyanogenesis ; β-glucosidase ; RFLP ; Trifolium repens
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract The Li locus in white clover controls the presence of cyanogenic β-glucosidase (linamarase) activity in leaf tissue, such that plants homozygous for the ‘null’ allele (li) have no linamarase activity in this tissue. The isolation of a cDNA clone from linamarase mRNA is described. The cDNA clone is used to further characterise alleles of the Li locus. Northern blot analysis shows that plants homozygous for the ‘null’ allele (li li) produce very reduced levels of mRNA which hybridises to the cDNA. Heterozygous plants (Li li), which have intermediate levels of enzyme activity, produce intermediate levels of mRNA. Southern blot analysis of Hind III digested genomic DNA shows that the white clover genome contains three genes with homology to the linamarase cDNA and that at least two of these genes segregate independently. Analysis of the cosegregation of linamarase activity and the presence of genomic restriction fragments identifies the genomic sequence specifying linamarase structure and indicates either a structural or cis acting control function of the Li locus.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00028776
    Standort Signatur Erwartet Verfügbarkeit
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    Artikel (Nationallizenzen)
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  • 4
    Digitale Medien
    Digitale Medien
    Nucleotide sequence and molecular analysis of the low temperature induced cereal gene, BLT4 (1991)
    Springer
    Molecular genetics and genomics 229 (1991), S. 389-394 
    Details
    ISSN: 1617-4623
    Schlagwort(e): Barley ; cDNA ; Cold ; Gene expression ; Low temperature
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary The nucleotide sequence and derived amino acid sequence of a cDNA clone (BLT4) for a low temperature induced barley gene were determined. This gene, together with a small family of related genes, was shown to reside on chromosome 3. The BLT4 clone has homology with genes in wheat and oats. Its expression was studied in oats and in barley doubled haploid lines segregating for spring/winter habit and for frost hardiness. These analyses show that elevated steady state levels of BLT4 mRNA are produced in shoot meristematic tissue after 3 days low positive temperature treatment. The low temperature response was found in all barley doubled haploid lines and was therefore not associated specifically with either the spring/winter habit or frost hardiness. Elevated levels of BLT4 mRNA were also seen in drought-stressed barley and it is likely that this is a gene encoding a low molecular weight protein that is responsive to dehydrative stresses, such as cold and drought.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00267460
    Standort Signatur Erwartet Verfügbarkeit
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    Artikel (Nationallizenzen)
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