Publikationsdatum:
2014-07-01
Beschreibung:
Background: Chronic inflammation-mediated beta-cell apoptosis is known to decrease beta-cell mass in diabetes leading to reduced insulin secretion. Exposure to pro-inflammatory cytokines can stimulate apoptosis in pancreatic beta-cells. The G protein coupled receptor 40 (GPR40) is implicated for glucose induced insulin secretion. We hypothesized that GPR40 activation can protect beta-cells from inflammation-induced apoptosis and restore glucose stimulated insulin secretion. Results: By exposing NIT1 insulinoma cells and rat islets to a cocktail of pro-inflammatory cytokines (TNFalpha and IL1beta), we mimicked inflammatory signaling as seen by JNK and NFkappaB activation and increased mRNA levels of TNFalpha, IL1beta and NOS2a. These changes were reversed by pharmacological activation of GPR40 by a specific, small molecule, CNX-011-67. Further, GPR40 activation reduced inflammation-mediated oxidative and endoplasmic reticulum (ER) stresses. Importantly, GPR40 activation decreased inflammation-induced apoptosis as measured by key markers. These impacts of GPR40 were mediated through activation of PLC, CaMKII, calcineurin and cAMP. Cell survival was also enhanced by GPR40 activation as seen from the increased phosphorylation of Akt/PKB and enhanced expression of BCL2 and PDX1 genes. Interestingly, GPR40 activation restored both, inflammation-mediated inhibition on insulin secretion and intracellular insulin content. Conclusions: In this study, we provide evidences that CNX-011-67, a GPR40 agonist, reduces inflammatory signaling and apoptosis in pancreatic beta-cell while promoting insulin secretion and synthesis. Activation of GPR40 leads to attenuation of beta-cell dysfunction caused by chronic inflammation and thus could be of immense clinical value to improve insulin secretion and beta-cell survival.
Digitale ISSN:
1471-2121
Thema:
Biologie
,
Medizin
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